Phenotypes associated with this allele

• Allele Symbol: Ptk2^tm1Lfr
• Allele Name: targeted mutation 1, Louis F Reichardt
• Allele ID: MGI:2684666
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ptk2^tm1Lfr/Ptk2^tm1Ilic Tg(KRT5-cre)5132Jlj/0	involves: 129X1/SvJ * C57BL/6 * CBA * DBA/2J	MGI:3625475
cn2	Ptk2^tm1Lfr/Ptk2^tm1Lfr Tg(Tek-cre)1Rwng/0	involves: 129X1/SvJ * FVB/N	MGI:3689988
cx3	Fyn^tm1Yik/Fyn^tm1Yik Ptk2^tm1Lfr/Ptk2^+	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:3693516

Genotype
MGI:3625475

cn1

• Allelic Composition: Ptk2^tm1Lfr/Ptk2^tm1Ilic; Tg(KRT5-cre)5132Jlj/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA * DBA/2J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

decreased sebaceous gland number ( J:107206 )

• a significant deficit in sebaceous glands in 2-4 month-old mice

integument

decreased keratinocyte proliferation ( J:107206 )

• primary keratinocytes isolated from mutant animals would not proliferate in culture

decreased sebaceous gland number ( J:107206 )

• a significant deficit in sebaceous glands in 2-4 month-old mice

sparse hair ( J:107206 )

• from approximately postnatal day 7 until P17, initial hair growth was sparse

• by P17, the pelage of mutant mice appeared identical to their normal littermates

abnormal hair follicle orientation ( J:107206 )

• hair follicles are orientated randomly, rather than being arrayed in a hexagonal pattern as in control in P12 mice

decreased hair follicle number ( J:107206 )

• have consistently about 25% fewer hair follicles than control

abnormal hair cycle ( J:107206 )

• irregular hair cycle suggested by the presence of hair follicles in the hypodermis of P22 mice

• adult mutant mice periodically showed patches of receding hair, which lasted for several days, disappeared, and then appeared again at another location

thin epidermis ( J:107206 )

• a thinner epidermis was evident in mutant mice at all ages

cellular

decreased keratinocyte proliferation ( J:107206 )

• primary keratinocytes isolated from mutant animals would not proliferate in culture

Genotype
MGI:3689988

cn2

• Allelic Composition: Ptk2^tm1Lfr/Ptk2^tm1Lfr; Tg(Tek-cre)1Rwng/0
• Genetic Background: involves: 129X1/SvJ * FVB/N

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:104401 )

• lethality between E10.5 and E11.5

growth/size/body

decreased embryo size ( J:104401 )

• slightly smaller at E10.5

embryo

abnormal vitelline vasculature morphology ( J:104401 )

• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5

• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels

disorganized yolk sac vascular plexus ( J:104401 )

• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections

decreased embryo size ( J:104401 )

• slightly smaller at E10.5

cardiovascular system

abnormal blood vessel morphology ( J:104401 )

• show patches of sequestered blood, reflecting dilated vessels, primarily in the upper trunk and head regions

• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries

• para-aortic splanchnopleural mesoderm explants undergo a process in which endothelial cells contract and cluster with each other, resulting in an irregular network composed of wider and smaller vessels

abnormal vascular endothelial cell morphology ( J:104401 )

• isolated endothelial cells grown on fibronectin are poorly spread, lack membrane ruffling and lamellipodia formation and show aberrant locomotion that is faster in the presence of serum and growth supplements

• endothelial cells show fewer and abnormal stress fibers that instead resemble cortical actin bundles and show fewer focal adhesions

abnormal capillary morphology ( J:104401 )

• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries, leading to a great variation in the size of the capillaries and intercapillary spaces

• allantoic explants and para-aortic splanchnopleural mesoderm explants show dilation of capillaries and intercapillary spaces with a reduction in network complexity

abnormal vascular regression ( J:104401 )

• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression

decreased angiogenesis ( J:104401 )

• exhibit complete absence of blood vessels in the neuroepithelium at E10.5, indicating defective sprouting angiogenesis into the neuroepithelium

abnormal vitelline vasculature morphology ( J:104401 )

• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5

• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels

disorganized yolk sac vascular plexus ( J:104401 )

• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections

hemorrhage ( J:104401 )

• hemorrhage is apparent in both the amniotic and yolk sac cavities

cellular

abnormal vascular regression ( J:104401 )

• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression

Genotype
MGI:3693516

cx3

• Allelic Composition: Fyn^tm1Yik/Fyn^tm1Yik; Ptk2^tm1Lfr/Ptk2⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

immune system

thymus cortex atrophy ( J:110771 )

• atrophy of the thymic cortex

decreased thymocyte number ( J:110771 )

• about 2/3 of mutants start to show a decrease in the number of thymocytes after 3 weeks of age, with numbers dropping as low as 1/100 of normal at 4 to 5 weeks of age

abnormal T cell differentiation ( J:110771 )

• impaired development of CD4+CD8+ thymocytes

decreased double-positive T cell number ( J:110771 )

• exhibit a reduction in double-positive thymocytes coincident with cortical atrophy

hematopoietic system

thymus cortex atrophy ( J:110771 )

• atrophy of the thymic cortex

decreased thymocyte number ( J:110771 )

• about 2/3 of mutants start to show a decrease in the number of thymocytes after 3 weeks of age, with numbers dropping as low as 1/100 of normal at 4 to 5 weeks of age

abnormal T cell differentiation ( J:110771 )

• impaired development of CD4+CD8+ thymocytes

decreased double-positive T cell number ( J:110771 )

• exhibit a reduction in double-positive thymocytes coincident with cortical atrophy

endocrine/exocrine glands

thymus cortex atrophy ( J:110771 )

• atrophy of the thymic cortex

decreased thymocyte number ( J:110771 )

• about 2/3 of mutants start to show a decrease in the number of thymocytes after 3 weeks of age, with numbers dropping as low as 1/100 of normal at 4 to 5 weeks of age