Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-EGFP)1Osb
• Allele Name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
• Allele ID: MGI:2686773
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Ccr5^tm1Kuz/Ccr5^tm1Kuz Tg(CAG-EGFP)1Osb/?	involves: 129P2/OlaHsd * C3H * C57BL/6	MGI:5317844
cx2	Cryaa^2J/Cryaa^2J Tg(CAG-EGFP)1Osb/0	involves: BALB/c * C3H * C57BL/6 * FVB/N	MGI:3690119
cx3	Cryaa^L1N/Cryaa^L1N Tg(CAG-EGFP)1Osb/0	involves: C3H * C57BL/6	MGI:3690120
cx4	Tg(CAG-EGFP)1Osb/0 Tg(Rho)1Wbae/0	involves: C57BL/6 * SJL	MGI:4366757
tg5	Tg(CAG-EGFP)1Osb/Tg(CAG-EGFP)1Osb	C57BL/6-Tg(CAG-EGFP)1Osb/J	MGI:3531413
tg6	Tg(CAG-EGFP)1Osb/0	C57BL/6-Tg(CAG-EGFP)1Osb/J	MGI:2686900

Genotype
MGI:5317844

cx1

• Allelic Composition: Ccr5^tm1Kuz/Ccr5^tm1Kuz; Tg(CAG-EGFP)1Osb/?
• Genetic Background: involves: 129P2/OlaHsd * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

decreased metastatic potential ( J:97833 )

• 40.2 metastic colonies compared to 70.6 colonies for controls 14 days after injecting B16-F10 melanoma cells

• fewer metastatic tumors 14 days after injecting irradiated mice with melanoma cells

• injection of wild type lung stromal cells enhanced metastasis relative to injection of mutant stromal cells

• improved survival

Genotype
MGI:3690119

cx2

• Allelic Composition: Cryaa^2J/Cryaa^2J; Tg(CAG-EGFP)1Osb/0
• Genetic Background: involves: BALB/c * C3H * C57BL/6 * FVB/N

vision/eye

abnormal lens morphology ( J:112248 )

• peripheral fiber cells of E16.5 lenses contain degenerated mitochondria with severe loss of matrix and cristae or with vacuoles

• severely disrupted actin filaments are seen in contact regions between anterior epithelial cells and posterior fiber cells in lenses at E16.5

abnormal lens capsule morphology ( J:112248 )

• altered epithelial cells and degenerated fiber cells are seen in lenses; epithelial cells are detached from or aggregated beneath the lense capsule and posterior fiber cells are only partially elongated

abnormal lens epithelium morphology ( J:112248 )

• epithelium is severely altered; epithelial layer contains irregularly shaped cells and vesicle-like cellular structures, thus lacking polarized monolayer of cuboidal epithelial cells morphology seen in wild-type

• severely disrupted actin filaments are seen in contact regions between anterior epithelial cells and posterior fiber cells in lenses at E16.5

Genotype
MGI:3690120

cx3

• Allelic Composition: Cryaa^L1N/Cryaa^L1N; Tg(CAG-EGFP)1Osb/0
• Genetic Background: involves: C3H * C57BL/6

vision/eye

vision/eye phenotype ( J:112248 )

N • histology shows no obvious changes in lens epithelial and fiber cells at weaning age

abnormal lens morphology ( J:112248 )

• analysis shows that mutant lenses contain a greater amount of water-insoluble crystallin proteins, likely correlating with the nuclear cataracts observed in mice

Genotype
MGI:4366757

cx4

• Allelic Composition: Tg(CAG-EGFP)1Osb/0; Tg(Rho)1Wbae/0
• Genetic Background: involves: C57BL/6 * SJL

nervous system

retina photoreceptor degeneration ( J:87298 )

• double mutants exhibit the same level of retinal degeneration as is observed in hemizygous Tg(Rho)1Wbae mice

vision/eye

retina photoreceptor degeneration ( J:87298 )

• double mutants exhibit the same level of retinal degeneration as is observed in hemizygous Tg(Rho)1Wbae mice

Genotype
MGI:3531413

tg5

• Allelic Composition: Tg(CAG-EGFP)1Osb/Tg(CAG-EGFP)1Osb
• Genetic Background: C57BL/6-Tg(CAG-EGFP)1Osb/J

mortality/aging

prenatal lethality, complete penetrance ( J:87298 )

• homozygous transgenic mice die in the embryonic stage, putatively due to integration site effect

Genotype
MGI:2686900

tg6

• Allelic Composition: Tg(CAG-EGFP)1Osb/0
• Genetic Background: C57BL/6-Tg(CAG-EGFP)1Osb/J

normal phenotype

no abnormal phenotype detected ( J:87298 , J:87415 )

• mice are viable and show no functional or morphological abnormalities of the retina (J:87298)

• all tissues, except for erytrhocytes and hair, are green under excitation light (J:87415)