Phenotypes associated with this allele

• Allele Symbol: Pax3^tm1Buck
• Allele Name: targeted mutation 1, Margaret E Buckingham
• Allele ID: MGI:2687297
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pax3^tm1Buck/Pax3^tm1Buck	involves: 129P2/OlaHsd	MGI:2687391
hm2	Pax3^tm1Buck/Pax3^tm1Buck	involves: 129P2/OlaHsd * C57BL/6	MGI:3629788
ht3	Pax3^tm1Buck/Pax3^+	involves: 129P2/OlaHsd	MGI:2687392
ht4	Pax3^tm1Buck/Pax3^Sp	involves: 129P2/OlaHsd * C57BL	MGI:2687398
cx5	Pax3^tm1Buck/Pax3^tm1Buck Pax7^tm2Pgr/Pax7^tm2Pgr	either: (involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ) or (involves: 129P2/OlaHsd * 129S2/SvPas)	MGI:3603041

Genotype
MGI:2687391

hm1

• Allelic Composition: Pax3^tm1Buck/Pax3^tm1Buck
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

nervous system

abnormal neural tube morphology ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

spina bifida ( J:176192 )

• in all mice

exencephaly ( J:176192 )

• in 50% of mice

abnormal dorsal root ganglion morphology ( J:176192 )

• at E12.5, axons and spinal ganglia are severely reduced compared to in control mice

muscle

abnormal muscle precursor cell migration ( J:176192 )

• absent in forelimbs at E10.5

abnormal muscle development ( J:176192 )

• in the limb

abnormal myogenesis ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

abnormal myotome development ( J:176192 )

abnormal dermomyotome development ( J:176192 )

• in the epaxial and hypaxial dermomyotome

embryo

abnormal neural crest cell migration ( J:176192 )

• at E12.5, axons and spinal ganglia are severely reduced compared to in control mice

abnormal neural tube morphology ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

spina bifida ( J:176192 )

• in all mice

abnormal somite development ( J:86911 , J:176192 )

• somites are disorganized and truncated in the hypaxial and epaxial domains (J:86911)

• disorganized (J:176192)

cellular

abnormal muscle precursor cell migration ( J:176192 )

• absent in forelimbs at E10.5

abnormal neural crest cell migration ( J:176192 )

• at E12.5, axons and spinal ganglia are severely reduced compared to in control mice

Genotype
MGI:3629788

hm2

• Allelic Composition: Pax3^tm1Buck/Pax3^tm1Buck
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

embryo

abnormal somite development ( J:109476 )

• mice lack hypaxial buds of somites 11-24

endocrine/exocrine glands

abnormal mammary placode morphology ( J:109476 )

♀ • placode 3 forms a day late compared to the other placodes

abnormal mammary line morphology ( J:109476 )

♀ • mammary line is located at the level of the notochord in mutants but more ventrally in controls embryos

♀ • narrower mammary line in mutants correlates with a band of multilayered ectoderm that contains one cell layer less than in controls

integument

abnormal mammary placode morphology ( J:109476 )

♀ • placode 3 forms a day late compared to the other placodes

abnormal mammary line morphology ( J:109476 )

♀ • mammary line is located at the level of the notochord in mutants but more ventrally in controls embryos

♀ • narrower mammary line in mutants correlates with a band of multilayered ectoderm that contains one cell layer less than in controls

Genotype
MGI:2687392

ht3

• Allelic Composition: Pax3^tm1Buck/Pax3⁺
• Genetic Background: involves: 129P2/OlaHsd

pigmentation

belly spot ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

integument

belly spot ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

Genotype
MGI:2687398

ht4

• Allelic Composition: Pax3^tm1Buck/Pax3^Sp
• Genetic Background: involves: 129P2/OlaHsd * C57BL

mortality/aging

prenatal lethality, complete penetrance ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice; however, no data is provided in J:86911

nervous system

open neural tube ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice

exencephaly ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice

• incidence is less than the incidence of spina bifida

absent dorsal root ganglion ( J:86911 )

muscle

abnormal myogenesis ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice including absence of limb muscles at E11.5

abnormal dermomyotome development ( J:86911 )

• stated to display the same phenotype as Pax3^Sp

embryo

abnormal neural crest cell migration ( J:86911 )

• impaired

open neural tube ( J:86911 )

• stated to display the same phenotype as Pax3^Sp mice

cellular

abnormal neural crest cell migration ( J:86911 )

• impaired

Genotype
MGI:3603041

cx5

• Allelic Composition: Pax3^tm1Buck/Pax3^tm1Buck; Pax7^tm2Pgr/Pax7^tm2Pgr
• Genetic Background: either: (involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ) or (involves: 129P2/OlaHsd * 129S2/SvPas)

mortality/aging

prenatal lethality, complete penetrance ( J:99365 )

• double homozygotes die at mid-gestation

muscle

abnormal myotome development ( J:99365 )

• at E11.5 there is a significant decrease in the differentiated muscle cells and by E13.5 only a few muscle fibers are present

• increased apoptosis is seen at E11.5 unlike in Pax3 single homozygotes

• progenitor cells at later embryonic and fetal stages are not specified as skeletal muscle