Phenotypes associated with this allele

• Allele Symbol: Efnb3^tm1Henk
• Allele Name: targeted mutation 1, Mark Henkemeyer
• Allele ID: MGI:3026700
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Efnb3^tm1Henk/Efnb3^tm1Henk	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)	MGI:3026744
hm2	Efnb3^tm1Henk/Efnb3^tm1Henk	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:3851499

Genotype
MGI:3026744

hm1

• Allelic Composition: Efnb3^tm1Henk/Efnb3^tm1Henk
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired coordination ( J:87497 )

• mutants are unable to perform asymmeteric movements about 3 days after birth and instead show synchronous mirror movements, for example, during grooming, when scratching the head with one hindlimb, the other hindlimb moves simultanously with the working limb and scratches the air

abnormal gait ( J:87497 )

• mirrored limb movement resulting in a hopping locomotion

nervous system

abnormal innervation ( J:87497 )

• corticospinal tract axons show an abnormal projection pattern in the spinal cord; collateral fibers branch into either the contralateral or ipsilateral side of the spinal cord gray matter

abnormal corticospinal tract morphology ( J:87497 )

• axons aberrantly cross the midline boundary of the spinal cord and bilaterally innervate both contralateral and ipsilateral motor neuron populations

Genotype
MGI:3851499

hm2

• Allelic Composition: Efnb3^tm1Henk/Efnb3^tm1Henk
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CD-1

nervous system

abnormal axon pruning ( J:150550 )

• mossy fiber pruning is defective

• dentate granule cells cocultured with Cos-1 cells exhibit longer neurites than similarly treated wild-type cells due to defective pruning

• however, transfection with fusion of EB3-Grb4-SH3 restores neurite pruning activity in culture

abnormal axon morphology ( J:150550 )

• infra-pyramidal bundle (IPB) axons extend further into the CA3 area underneath the pyramidal neurons than in wild-type mice

• IPB length does not decrease with age unlike in wild-type mice

cellular

abnormal axon pruning ( J:150550 )

• mossy fiber pruning is defective

• dentate granule cells cocultured with Cos-1 cells exhibit longer neurites than similarly treated wild-type cells due to defective pruning

• however, transfection with fusion of EB3-Grb4-SH3 restores neurite pruning activity in culture