All Phenotypes Anapc2<tm2Kna> MGI Mouse

premature death ( J:87782 )

• most die during the second week after the first pI/C injection to induce cre-mediated deletion of Anapc2 in the liver and lymphocytes

decreased liver glycogen level ( J:87782 )

• hepatocytes of mutants treated with pI/C lack glycogen

abnormal hepatocyte morphology ( J:87782 )

• hepatocytes are greatly enlarged, lack nuclear membranes, and contain condensed chromosomes in 6 of 10 mutants after pI/C injection

• 72% of mitotic hepatocytes are in a prometaphase-like state with condensed chromatin

abnormal liver physiology ( J:87782 )

• levels of the liver enzymes alanine aminotransferase and glutamate dehydrogenase are highly elevated in pI/C injected mutants, indicating functional impairment and damage of hepatocytes

increased hepatocyte proliferation ( J:87782 )

• quiescent hepatocytes of some but not all livers enter into a proliferative state, resulting in a mitotic arrest

• 2/3 hepatectomy in mutants transplanted with wild-type bone marrow and treated with pI/C caused 70% of hepatoctyes to re-enter the cell cycle and embark on proliferation and arrest in metaphase 3 to 5 days after hepatectomy

liver failure ( J:87782 )

• die from acute liver failure after pI/C injection

anemia ( J:87782 )

• the 4 of 10 mutants injected with pI/C with normal livers and 2 of 6 with abnormal livers show severe anemia

decreased hemoglobin content ( J:87782 )

• the 4 of 10 mutants injected with pI/C with normal livers show decreased hemoglobin levels

increased circulating bilirubin level ( J:87782 )

• levels of bilirubin are highly elevated in pI/C injected mutants

abnormal enzyme/coenzyme level ( J:87782 )