mortality/aging
• die before E6.5 as no homozygous embryos are found at E6.5 or E9.5
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Allele Symbol Allele Name Allele ID |
Anapc2tm2Kna targeted mutation 2, Kim Nasmyth MGI:3029826 |
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Summary |
2 genotypes
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
• die before E6.5 as no homozygous embryos are found at E6.5 or E9.5
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
• most die during the second week after the first pI/C injection to induce cre-mediated deletion of Anapc2 in the liver and lymphocytes
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• hepatocytes of mutants treated with pI/C lack glycogen
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• hepatocytes are greatly enlarged, lack nuclear membranes, and contain condensed chromosomes in 6 of 10 mutants after pI/C injection
• 72% of mitotic hepatocytes are in a prometaphase-like state with condensed chromatin
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• levels of the liver enzymes alanine aminotransferase and glutamate dehydrogenase are highly elevated in pI/C injected mutants, indicating functional impairment and damage of hepatocytes
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• quiescent hepatocytes of some but not all livers enter into a proliferative state, resulting in a mitotic arrest
• 2/3 hepatectomy in mutants transplanted with wild-type bone marrow and treated with pI/C caused 70% of hepatoctyes to re-enter the cell cycle and embark on proliferation and arrest in metaphase 3 to 5 days after hepatectomy
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• die from acute liver failure after pI/C injection
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• the 4 of 10 mutants injected with pI/C with normal livers and 2 of 6 with abnormal livers show severe anemia
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• the 4 of 10 mutants injected with pI/C with normal livers show decreased hemoglobin levels
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• levels of bilirubin are highly elevated in pI/C injected mutants
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• levels of the liver enzymes alanine aminotransferase and glutamate dehydrogenase are highly elevated in pI/C injected mutants, indicating functional impairment and damage of hepatocytes
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• hepatocytes of mutants treated with pI/C lack glycogen
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• develop severe bone marrow aplasia within 4 days of pI/C injection; by day 4, majority of nucleated cells disappear from the bone marrow which contains mainly erythrocytes and a few lymphocytes
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• quiescent hepatocytes of some but not all livers enter into a proliferative state, resulting in a mitotic arrest
• 2/3 hepatectomy in mutants transplanted with wild-type bone marrow and treated with pI/C caused 70% of hepatoctyes to re-enter the cell cycle and embark on proliferation and arrest in metaphase 3 to 5 days after hepatectomy
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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