Phenotypes associated with this allele

• Allele Symbol: Bub1b^{Gt(neo-btk)1Dai}
• Allele Name: gene trap 1, Wei Dai
• Allele ID: MGI:3033386
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bub1b^Gt(neo-btk)1Dai/Bub1b^Gt(neo-btk)1Dai	involves: 129S4/SvJae * C57BL/6	MGI:3033402
ht2	Bub1b^Gt(neo-btk)1Dai/Bub1b^+	involves: 129S4/SvJae	MGI:3033412
ht3	Bub1b^Gt(neo-btk)1Dai/Bub1b^+	involves: 129S4/SvJae * C57BL/6	MGI:3033403

Genotype
MGI:3033402

hm1

• Allelic Composition: Bub1b^{Gt(neo-btk)1Dai}/Bub1b^{Gt(neo-btk)1Dai}
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and placentation, complete penetrance ( J:88223 )

• embryos die due to extensive apoptosis during early gestation, failing to survive beyond E8.5

• at E6.5, embryos either have developed abnormally or are resorbed

cellular

increased embryonic tissue cell apoptosis ( J:88223 )

• extensive apoptosis in embryos at E8.5

embryo

increased embryonic tissue cell apoptosis ( J:88223 )

• extensive apoptosis in embryos at E8.5

abnormal blastocyst morphology ( J:88223 )

• E3.5 blastocysts cultured in vitro fail to exhibit robust proliferation and become atrophic by the equivalent of E6.5

Genotype
MGI:3033412

ht2

• Allelic Composition: Bub1b^{Gt(neo-btk)1Dai}/Bub1b⁺
• Genetic Background: involves: 129S4/SvJae

neoplasm

increased colon adenoma incidence ( J:88094 )

• increased incidence after administration of azoxymethane

increased intestinal adenocarcinoma incidence ( J:88094 )

• increased incidence after administration of azoxymethane

cellular

abnormal mitotic spindle assembly checkpoint ( J:88094 )

• in vitro studies showed impaired mitotic arrest due to defective spindle checkpoint activation

digestive/alimentary system

increased colon adenoma incidence ( J:88094 )

• increased incidence after administration of azoxymethane

increased intestinal adenocarcinoma incidence ( J:88094 )

• increased incidence after administration of azoxymethane

Genotype
MGI:3033403

ht3

• Allelic Composition: Bub1b^{Gt(neo-btk)1Dai}/Bub1b⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

embryo

embryo phenotype ( J:88223 )

N • in contrast to homozygotes, heterozygous blastocysts developed normally

hematopoietic system

abnormal megakaryocyte differentiation ( J:88223 )

• increased number of megakaryocytes

• defect in the formation of proplatelet-producing megakaryocytes

• no significant increase in the number of platelets in the peripheral blood

enlarged spleen ( J:88223 )

• due to increase in the number of splenic megakaryocytes

abnormal definitive hematopoiesis ( J:88223 )

absent common myeloid progenitor cells ( J:88223 )

• decreased number of erythroid progenitors

• the number of megakaryocytic progenitors was not decreased, but rather increased

decreased erythrocyte cell number ( J:88223 )

immune system

enlarged spleen ( J:88223 )

• due to increase in the number of splenic megakaryocytes

cellular

abnormal megakaryocyte differentiation ( J:88223 )

• increased number of megakaryocytes

• defect in the formation of proplatelet-producing megakaryocytes

• no significant increase in the number of platelets in the peripheral blood

growth/size/body

enlarged spleen ( J:88223 )

• due to increase in the number of splenic megakaryocytes