Phenotypes associated with this allele

• Allele Symbol: Ifng^tm1Yiw
• Allele Name: targeted mutation 1, Yoichiro Iwakura
• Allele ID: MGI:3036127
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ifng^tm1Yiw/Ifng^tm1Yiw	B6.129X1-Ifng^tm1Yiw	MGI:6430478
hm2	Ifng^tm1Yiw/Ifng^tm1Yiw	C.129X1-Ifng^tm1Yiw	MGI:6430488
hm3	Ifng^tm1Yiw/Ifng^tm1Yiw	involves: 129X1/SvJ * C57BL/6	MGI:3036135

Genotype
MGI:6430478

hm1

• Allelic Composition: Ifng^tm1Yiw/Ifng^tm1Yiw
• Genetic Background: B6.129X1-Ifng^tm1Yiw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

peritoneal inflammation ( J:50405 )

• JHMV infected mice develop peritonitis at 21 dpi

• JHMV infection is characterized by disseminated granulomatous inflammation and exudative fibrinous serositis with plasma cells and eosinophils in the abdominal cavity and these same lesions in the thoracic cavity

abnormal circulating interferon level ( J:91691 )

• JHMV infected mice show increased levels of IFN activity; similar level of IFN activity is seen on the C57BL/6 background as on BALB/c background

liver inflammation ( J:50405 )

• mice injected with an anti-CD8 monoclonal antibody to deplete CD8+ T cells and then infected with JHMV develop severe hepatitis and die within 2 weeks

• however, JHMV infected mice without depletion of CD8+ T cells do not develop hepatitis

increased susceptibility to Coronaviridae infection ( J:50405 , J:91691 )

• mice show increased susceptibility to infection with murine coronavirus strain JHM (hepatitis mouse virus) , showing higher viral titers at 3 and 5 dpi with a gradual decrease but persistence of virus in the liver compared to viral clearing in the liver of controls, higher antibody titers and virus neutralization antibody tiers (J:50405)

• virus is recovered from various tissues, including the liver, spleen, kidney, pancreas, mesenterium and lung of some JHMV-infected mice at 21 dpi, with the highest level of viral growth in the mesenterium (J:50405)

• although both cell-mediated and humoral immune responses are activated during JHMV infection, JHMV persists in mice (J:50405)

• Background Sensitivity: viral titer of JHMV infected mice is slightly higher than in heterozygotes on a C57BL/6 background compared to a much higher viral titer in mice on a BALB/c background (J:91691)

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:50405 , J:91691 )

• JHMV infected mice show some lethality at 16 days post infection (dpi), with survival rate gradually decreasing such that about 90% die by 50 dpi (J:50405)

• JHMV-infected mice treated with recombinant IFN-gamma show increases survival time (J:50405)

• Background Sensitivity: mice infected with the DL variant of the coronavirus mouse hepatitis virus (JHMV) show a gradual decrease in survival rate on the C57BL/6 background compared to lethality within 1 week on a BALB/c background (J:91691)

mortality/aging

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:50405 , J:91691 )

• JHMV infected mice show some lethality at 16 days post infection (dpi), with survival rate gradually decreasing such that about 90% die by 50 dpi (J:50405)

• JHMV-infected mice treated with recombinant IFN-gamma show increases survival time (J:50405)

• Background Sensitivity: mice infected with the DL variant of the coronavirus mouse hepatitis virus (JHMV) show a gradual decrease in survival rate on the C57BL/6 background compared to lethality within 1 week on a BALB/c background (J:91691)

digestive/alimentary system

peritoneal inflammation ( J:50405 )

• JHMV infected mice develop peritonitis at 21 dpi

• JHMV infection is characterized by disseminated granulomatous inflammation and exudative fibrinous serositis with plasma cells and eosinophils in the abdominal cavity and these same lesions in the thoracic cavity

liver/biliary system

liver inflammation ( J:50405 )

• mice injected with an anti-CD8 monoclonal antibody to deplete CD8+ T cells and then infected with JHMV develop severe hepatitis and die within 2 weeks

• however, JHMV infected mice without depletion of CD8+ T cells do not develop hepatitis

abnormal liver morphology ( J:50405 )

• JHMV infected mice show larger liver lesions that are infiltrated with neutrophils compared to wild-type infected mice

• some JHMV infected mice exhibit pseudomembrane on the surface of the liver at 14 dpi

• however, JHMV-infected mice do not develop severe hepatitis and alanine transaminase levels are similar to those in infected wild-type mice

focal hepatic necrosis ( J:91691 )

• Background Sensitivity: JHMV infected mice show only focal necrotic lesion in the liver at 5 days post infection (dpi) on a C57BL/6 background compared to massive liver necrosis on a BALB/c background

homeostasis/metabolism

abnormal circulating interferon level ( J:91691 )

• JHMV infected mice show increased levels of IFN activity; similar level of IFN activity is seen on the C57BL/6 background as on BALB/c background

ascites ( J:50405 )

• about 50% of JHMV infected mice exhibit accumulation of ascites fluid at P21

• however, no bacteria is isolated from ascites fluid

growth/size/body

abnormal abdominal wall morphology ( J:50405 )

• some JHMV infected mice exhibit organs in abdominal cavities that adhere to each other and the peritoneum

abnormal thoracic cavity morphology ( J:50405 )

• JHMV infected mice show accumulatio of a translucent fluid in the thoracic cavity in the subacute phase

behavior/neurological

abnormal behavior ( J:50405 )

• a small number of JHMV infected mice show signs of central nervous system disease such as inability to turn over rapidly

Genotype
MGI:6430488

hm2

• Allelic Composition: Ifng^tm1Yiw/Ifng^tm1Yiw
• Genetic Background: C.129X1-Ifng^tm1Yiw

immune system

abnormal circulating interferon level ( J:91691 )

• JHMV infected mice show increased levels of IFN activity; similar level of IFN activity is seen on the BALB/c background as on C57BL/6 background

increased susceptibility to Coronaviridae infection ( J:91691 )

• Background Sensitivity: viral titer of JHMV infected mice is 20- and 6-fold higher than in heterozygotes at 3 and 5 dpi, respectively, on a BALB/c background compared to a much lower viral titer in mice on a BALB/c background

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:91691 )

• Background Sensitivity: mice infected with the DL variant of the coronavirus mouse hepatitis virus (JHMV) die within 1 week after infection when on a BALB/c background compared to longer survival on a C57BL/6 background

• JHMV-infected mice treated with exogenous IFN-gamma show increased survival rate

mortality/aging

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:91691 )

• Background Sensitivity: mice infected with the DL variant of the coronavirus mouse hepatitis virus (JHMV) die within 1 week after infection when on a BALB/c background compared to longer survival on a C57BL/6 background

• JHMV-infected mice treated with exogenous IFN-gamma show increased survival rate

liver/biliary system

diffuse hepatic necrosis ( J:91691 )

• Background Sensitivity: JHMV infected mice show massive liver necrosis at 5 days post infection (dpi) on a BALB/c background compared to only focal necrotic lesion in mice on a C57BL/6 background

homeostasis/metabolism

abnormal circulating interferon level ( J:91691 )

• JHMV infected mice show increased levels of IFN activity; similar level of IFN activity is seen on the BALB/c background as on C57BL/6 background

increased circulating alanine transaminase level ( J:91691 )

• Background Sensitivity: JHMV infected mice show a much greater increase in serum ALT activity at 3 and 5 dpi on the BALB/c background compared to only a slight rise on the C57BL/6 background

Genotype
MGI:3036135

hm3

• Allelic Composition: Ifng^tm1Yiw/Ifng^tm1Yiw
• Genetic Background: involves: 129X1/SvJ * C57BL/6

cellular

decreased apoptosis ( J:41632 )

• Con A treatment lead to no apoptosis in the liver although apoptosis in the spleen was similar to that seen in control mice

immune system

decreased inflammatory response ( J:41632 , J:88226 )

• resistant to hepatitis induced by Con A injection (J:41632)

• attenuated enteritis resulting from exposure to Clostridium difficile Toxin A (J:88226)

liver/biliary system

hepatic necrosis ( J:41632 )

• unlike controls, few necrotic cells were found in livers after Con A treatment

reproductive system

abnormal female reproductive system physiology ( J:59892 )

♀ • alpha-galactosylceramide induced abortion does not occur