Phenotypes associated with this allele

• Allele Symbol: Junb^tm3Wag
• Allele Name: targeted mutation 3, Erwin F Wagner
• Allele ID: MGI:3036209
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Junb^tm3Wag/Junb^tm3Wag Meox2^tm1(cre)Sor/Meox2^+	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6	MGI:3036219
cn2	Junb^tm3Wag/Junb^tm3Wag Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3036221
cn3	Jun^tm4Wag/Jun^tm4Wag Junb^tm3Wag/Junb^tm3Wag Tg(Krt1-5-cre/ERT)1Ipc/0	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5578332
cn4	Il6^tm1Kopf/Il6^tm1Kopf Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak/0	involves: 129/Sv * 129P2/OlaHsd * 129S2/SvPas * C3H * C57BL/6	MGI:4417905
cn5	Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak/0	involves: 129/Sv * 129P2/OlaHsd * C3H * C57BL/6	MGI:4417904
cn6	Csf3^tm1Ard/Csf3^tm1Ard Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak/0	involves: 129/Sv * 129P2/OlaHsd * C3H * C57BL/6	MGI:4417906

Genotype
MGI:3036219

cn1

• Allelic Composition: Junb^tm3Wag/Junb^tm3Wag; Meox2^tm1(cre)Sor/Meox2⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased chronic myelocytic leukemia incidence ( J:88126 )

• myeloproliferative CML-like disease by 3-6 months of age

• massive infiltration of bone marrow and spleen with neutrophilic granulocytes

limbs/digits/tail

short tibia ( J:88126 )

• tibia length was reduced by 6 months

skeleton

decreased osteoclast cell number ( J:88126 )

• 70% reduction in numbers of osteoclasts

short tibia ( J:88126 )

• tibia length was reduced by 6 months

decreased bone mineral density ( J:88126 )

• severe osteopenia by 4 weeks of age wihich progressed with age

• bones were thin and brittle by 7 days of age

• by 1 month, vertebral bone mass was decreased

decreased compact bone thickness ( J:88126 )

• cortical bone thickness severely reduced at 6 months

decreased osteoblast cell number ( J:88126 )

• number of osteoblasts is reduced by 7 days

• osteoblast numbers reduced up to 90% by 6 months

abnormal skeleton development ( J:88126 )

abnormal osteoclast differentiation ( J:88126 )

• defective osteoclast differentiation

abnormal skeleton physiology ( J:88126 )

abnormal bone ossification ( J:88126 )

• 60% reduction in cortical bone formation at 1 month, 40% reduction at 3 months

• 70% reduction in trabecular bone formation at 1 month, 30% reduction at 3 months

decreased bone resorption ( J:88126 )

• bone resorption significantly reduced

decreased bone strength ( J:88126 )

• bone strength was significantly reduced

hematopoietic system

abnormal osteoclast differentiation ( J:88126 )

• defective osteoclast differentiation

decreased osteoclast cell number ( J:88126 )

• 70% reduction in numbers of osteoclasts

immune system

abnormal osteoclast differentiation ( J:88126 )

• defective osteoclast differentiation

decreased osteoclast cell number ( J:88126 )

• 70% reduction in numbers of osteoclasts

cellular

abnormal osteoclast differentiation ( J:88126 )

• defective osteoclast differentiation

Genotype
MGI:3036221

cn2

• Allelic Composition: Junb^tm3Wag/Junb^tm3Wag; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

skeleton

abnormal osteoclast morphology ( J:88126 )

• smaller mononuclear osteoclasts

abnormal trabecular bone morphology ( J:88126 )

• increased trabecular bone volume

• increased radiodensity

• irregular patterning of trabecular bone

abnormal bone ossification ( J:88126 )

hematopoietic system

abnormal osteoclast morphology ( J:88126 )

• smaller mononuclear osteoclasts

immune system

abnormal osteoclast morphology ( J:88126 )

• smaller mononuclear osteoclasts

Genotype
MGI:5578332

cn3

• Allelic Composition: Jun^tm4Wag/Jun^tm4Wag; Junb^tm3Wag/Junb^tm3Wag; Tg(Krt1-5-cre/ERT)1Ipc/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

integument

skin inflammation ( J:116380 )

• intra-epidermal T cells, epidermal micro-abscesses and typical inflammatory-cell infiltrate consisting of neutrophils, and an increase in numbers of macrophages in the dermis are seen in mice 8-10 days after tamoxifen induction

abnormal skin morphology ( J:116380 )

• mice treated with tamoxifen show an increase in subepidermal vascularization

hyperkeratosis ( J:116380 )

• thickening of keratinized upper layers in mice treated with tamoxifen

parakeratosis ( J:116380 )

• in mice treated with tamoxifen

thick epidermis ( J:116380 )

• mice treated with tamoxifen show thickened epidermis with prominent rete ridge

psoriasis ( J:116380 )

• mice treated with tamoxifen at 8 weeks of age develop psoriasis-like skin lesions on hairless skin 8-10 days after tamoxifen induction, showing scaly plaques affecting primarily ears, paws and tail, and less frequently the hairy back skin

immune system

increased inflammatory response ( J:116380 )

• periostitis in mice treated with tamoxifen

autoimmune arthritis ( J:116380 )

• arthritic lesions similar to psoriatic arthritis are seen in 100% of mice treated with tamoxifen, showing inflammatory infiltrates in joint regions

skin inflammation ( J:116380 )

• intra-epidermal T cells, epidermal micro-abscesses and typical inflammatory-cell infiltrate consisting of neutrophils, and an increase in numbers of macrophages in the dermis are seen in mice 8-10 days after tamoxifen induction

skeleton

autoimmune arthritis ( J:116380 )

• arthritic lesions similar to psoriatic arthritis are seen in 100% of mice treated with tamoxifen, showing inflammatory infiltrates in joint regions

abnormal bone structure ( J:116380 )

• massive bone destruction

abnormal periosteum morphology ( J:116380 )

• periostitis in mice treated with tamoxifen

cardiovascular system

abnormal blood vessel morphology ( J:116380 )

• mice treated with tamoxifen show an increase in subepidermal vascularization

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis		DOID:8893	OMIM:PS177900	J:116380

Genotype
MGI:4417905

cn4

• Allelic Composition: Il6^tm1Kopf/Il6^tm1Kopf; Junb^tm3Wag/Junb^tm3Wag; Tg(KRT5-cre)1Tak/0
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * 129S2/SvPas * C3H * C57BL/6

mortality/aging

mortality/aging ( J:155575 )

N • mice exhibit normal lifespan unlike Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice

immune system

immune system phenotype ( J:155575 )

N • mice exhibit a rescue of the systemic lupus erythematosus associated phenotypes observed in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice including normal lymph node architecture, plasma cell count, and antinuclear autoantibodies

skin inflammation ( J:155575 )

• mild

renal/urinary system

renal/urinary system phenotype ( J:155575 )

N • mice exhibit a rescue of the lupus associated kidney phenotypes observed in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:155575 )

N • ce exhibit normal urine albumin levels unlike in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice

integument

integument phenotype ( J:155575 )

N • mice exhibit a rescue of the lupus associated skin phenotypes observed in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice

skin inflammation ( J:155575 )

• mild

hyperkeratosis ( J:155575 )

• mild

thick skin ( J:155575 )

• milder than in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice

Genotype
MGI:4417904

cn5

• Allelic Composition: Junb^tm3Wag/Junb^tm3Wag; Tg(KRT5-cre)1Tak/0
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * C3H * C57BL/6

mortality/aging

premature death ( J:155575 )

• mice die before 20 months

immune system

increased plasma cell number ( J:155575 )

• mice exhibit an increase in plasma cells in the blood, lymph node and spleen unlike wild-type mice

increased IgE level ( J:155575 )

increased circulating interleukin-6 level ( J:155575 )

increased interleukin-6 secretion ( J:155575 )

• from epidermal cells

increased susceptibility to systemic lupus erythematosus ( J:155575 )

• mice exhibit skin ulcerations, the lupus band in the epidermal-dermal junction, increased IL-6 secretion, mesangial hypercellularity with glomerular basement membrane thickening and luminal obstruction, small and strophic kidney, increased anti-histone antibodies, and decreased survival compared with wild-type mice

increased anti-histone antibody level ( J:155575 )

increased inflammatory response ( J:155575 )

• UV-treated mice exhibit inflammatory perivascular infiltrates with abundant CD3+ T lymphocytes in the liver and lungs unlike similarly treated Junb^tm3Wag homozygotes

• UV-treated mice develop swelling of the paws with functional articular impairment, synovial proliferation, and synovial membrane inflammation unlike similarly treated Junb^tm3Wag homozygotes

glomerulonephritis ( J:155575 )

• unlike wild-type controls, mice develop immunocomplex glomerulonephritis (IC-GN) characterized by mesangial hypercellularity, lobulation of the glomerular tuft with basement membrane thickening, endocapillary hypercellularity, and luminal obstruction by immuno complex deposits

• UV-treated mice develop IC-GN accompanied by tubulo-interstitial nephritis and perivascular CD3+ T-cell-rich infiltrates unlike similarly treated Junb^tm3Wag homozygotes

tubulointerstitial nephritis ( J:155575 )

• UV-treated mice develop IC-GN accompanied by tubulo-interstitial nephritis and perivascular CD3+ T-cell-rich infiltrates unlike similarly treated Junb^tm3Wag homozygotes

dermatitis ( J:155575 )

• starting at 3 months, mice exhibit dermatitis of ears, snouts, upper thorax region, and paws unlike wild-type mice

renal/urinary system

abnormal glomerular capillary morphology ( J:155575 )

• mice display endocapillary hypercellularity and luminal obstruction by immuno complex deposits

increased mesangial cell number ( J:155575 )

• mice exhibit mesangial hypercellularity

albuminuria ( J:155575 )

glomerulonephritis ( J:155575 )

• unlike wild-type controls, mice develop immunocomplex glomerulonephritis (IC-GN) characterized by mesangial hypercellularity, lobulation of the glomerular tuft with basement membrane thickening, endocapillary hypercellularity, and luminal obstruction by immuno complex deposits

• UV-treated mice develop IC-GN accompanied by tubulo-interstitial nephritis and perivascular CD3+ T-cell-rich infiltrates unlike similarly treated Junb^tm3Wag homozygotes

tubulointerstitial nephritis ( J:155575 )

• UV-treated mice develop IC-GN accompanied by tubulo-interstitial nephritis and perivascular CD3+ T-cell-rich infiltrates unlike similarly treated Junb^tm3Wag homozygotes

podocyte foot process effacement ( J:155575 )

• mice exhibit podocyte foot process effacement in most glomerular capillary loops

increased renal glomerulus basement membrane thickness ( J:155575 )

• mice exhibit GBM thickening

increased renal glomerulus lobularity ( J:155575 )

• mice exhibit lobulation of the glomerular tuft

renal glomerular immunoglobulin deposits ( J:155575 )

small kidney ( J:155575 )

kidney atrophy ( J:155575 )

homeostasis/metabolism

increased circulating interleukin-6 level ( J:155575 )

albuminuria ( J:155575 )

hematopoietic system

increased plasma cell number ( J:155575 )

• mice exhibit an increase in plasma cells in the blood, lymph node and spleen unlike wild-type mice

increased IgE level ( J:155575 )

integument

dermatitis ( J:155575 )

• starting at 3 months, mice exhibit dermatitis of ears, snouts, upper thorax region, and paws unlike wild-type mice

abnormal epidermal-dermal junction morphology ( J:155575 )

• mice exhibit the lupus band in the epidermal-dermal junction unlike wild-type mice

spontaneous skin ulceration ( J:155575 )

• at 3 months

thick skin ( J:155575 )

cardiovascular system

abnormal glomerular capillary morphology ( J:155575 )

• mice display endocapillary hypercellularity and luminal obstruction by immuno complex deposits

cellular

increased mesangial cell number ( J:155575 )

• mice exhibit mesangial hypercellularity

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
systemic lupus erythematosus		DOID:9074	OMIM:152700 OMIM:300809 OMIM:605480 OMIM:608437 OMIM:609903 OMIM:609939 OMIM:610065 OMIM:610066 OMIM:612254 OMIM:612378 OMIM:613145 OMIM:614420	J:155575

Genotype
MGI:4417906

cn6

• Allelic Composition: Csf3^tm1Ard/Csf3^tm1Ard; Junb^tm3Wag/Junb^tm3Wag; Tg(KRT5-cre)1Tak/0
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * C3H * C57BL/6

immune system

increased susceptibility to systemic lupus erythematosus ( J:155575 )

• mice develop systemic lupus erythematosus associated phenotypes unlike wild-type mice

renal/urinary system

abnormal kidney morphology ( J:155575 )

• mice exhibit systemic lupus erythematosus associated kidney defects observed in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice

integument

abnormal epidermal layer morphology ( J:155575 )

• mice exhibit a partial rescue of epidermis defects observed in Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak mice