About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Runx1tm1Soga
targeted mutation 1, Seishi Ogawa
MGI:3036550
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Runx1tm1Soga/Runx1tm1.1Soga
Tg(Mx1-cre)1Cgn/0 		involves: C57BL/6 * CBA MGI:3036555
cn2
 		Runx1tm1Soga/Runx1tm1Soga
Stag2tm1.1Soga/Y
Tg(Mx1-cre)1Cgn/0 		involves: C57BL/6J * C57BL/6N * CBA/J MGI:6696300


Genotype
MGI:3036555
cn1
Allelic
Composition		 Runx1tm1Soga/Runx1tm1.1Soga
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm1.1Soga mutation (0 available); any Runx1 mutation (34 available)
Runx1tm1Soga mutation (0 available); any Runx1 mutation (34 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:88511 )
N
• mutants injected with pIpC to induce expression of Cre recombinase exhibit normal myeloid cell development, neutrophil counts, hemoglobin levels, and maintenance of hematopoietic stem cells in adults
abnormal common myeloid progenitor cell morphology ( J:88511 )
• pIpC treated mutants exhibit an increase in apoptosis of myeloid colony-forming cells
abnormal T cell differentiation ( J:88511 )
• pIpC treated mutants exhibit a significant block in the maturation of T-cell progenitors at the transition from CD44+CD25+ (double negative 2) to CD44-CD25+ (double negative 3), indicating the accumulation of immature T-cell precursors in the thymus
abnormal megakaryocyte morphology ( J:88511 )
• the immature megakaryocytes observed in bone marrow of mutants injected with pIpC exhibit poorly demarcated membranes and a lower level of polyploidy than controls
abnormal megakaryocyte differentiation ( J:88511 )
• after injection of pIpC to induce expression of Cre recombinase, bone marrow exhibits an arrest in megakaryocytic maturation
abnormal megakaryocyte progenitor cell morphology ( J:88511 )
• after injection of pIpC to induce expression of Cre recombinase, mutants exhibit an increase in hematopoietic progenitor cells
• bone marrow cells from pIpC injected mutants form more megakaryocytic (CFU-Meg), myeloid, and mixed (myeloid and erythroid) colonies in vitro than control bone marrow cells
thrombocytopenia ( J:88511 )
• immediately after injection of pIpC to induce expression of Cre recombinase, mice show a decline in platelet counts to 1/3 to 1/6 of those for the control

immune system
abnormal T cell differentiation ( J:88511 )
• pIpC treated mutants exhibit a significant block in the maturation of T-cell progenitors at the transition from CD44+CD25+ (double negative 2) to CD44-CD25+ (double negative 3), indicating the accumulation of immature T-cell precursors in the thymus

cellular
abnormal megakaryocyte differentiation ( J:88511 )
• after injection of pIpC to induce expression of Cre recombinase, bone marrow exhibits an arrest in megakaryocytic maturation




Genotype
MGI:6696300
cn2
Allelic
Composition		 Runx1tm1Soga/Runx1tm1Soga
Stag2tm1.1Soga/Y
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: C57BL/6J * C57BL/6N * CBA/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm1Soga mutation (0 available); any Runx1 mutation (34 available)
Stag2tm1.1Soga mutation (0 available); any Stag2 mutation (15 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
anemia ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection develop anemia with increased mean corpuscular volume and red blood cell distribution width
decreased leukocyte cell number ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection exhibit reduced white blood cell count, with a reduction in lymphocytes, granulocytes, and monocytes
increased hematopoietic stem cell number ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection exhibit increased frequency of immature bone marrow progenitors (LSK cells)
abnormal myeloid cell morphology ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection exhibit lineage-committed mature cells that are skewed to myeloid lineages
decreased erythroid progenitor cell number ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection show reduced frequency of colony-forming unit erythroid cells and Ter119+CD71+ erythroid progenitors
increased mean corpuscular volume ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection show increased mean corpuscular volume
increased red blood cell distribution width ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection show increased red blood cell distribution width
abnormal bone marrow cell physiology ( J:289000 )
• all lethally irradiated mice transplanted with mutant-derived bone marrow, followed by pIpC injection, develop overt myelodysplastic syndrome, mostly within half a year after pIpC injection, with severe cytopenia and marked trilineage dysplasia

immune system
decreased leukocyte cell number ( J:289000 )
• lethally irradiated mice transplanted with mutant bone marrow cells followed by pIpC injection exhibit reduced white blood cell count, with a reduction in lymphocytes, granulocytes, and monocytes




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory