All Phenotypes Srsf2<tm1Xdfu> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Myl2^tm1(cre)Krc/Myl2⁺ Srsf2^tm1Xdfu/Srsf2^tm1Xdfu	involves: 129S4/SvJae	MGI:3037209
cn2	Srsf2^tm1Xdfu/Srsf2^tm1Xdfu Tg(Lck-cre)I57Jxm/?	involves: 129S4/SvJae * 129X1/SvJ * ICR	MGI:3037198
cn3	Srsf2^tm1Xdfu/Srsf2⁺ Tg(Lck-cre)I57Jxm/?	involves: 129S4/SvJae * 129X1/SvJ * ICR	MGI:3037204
cn4	Srsf2^tm1Xdfu/Srsf2^tm1Xdfu Tg(Mx1-cre)1Cgn/?	involves: 129S4/SvJae * C57BL/6J * CBA/J	MGI:5695464

Allelic Composition	Myl2^tm1(cre)Krc/Myl2⁺ Srsf2^tm1Xdfu/Srsf2^tm1Xdfu
Genetic Background	involves: 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myl2^tm1(cre)Krc mutation (2 available); any Myl2 mutation (22 available)
Srsf2^tm1Xdfu mutation (1 available); any Srsf2 mutation (11 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

pregnancy-related premature death ( J:88432 )

• life span is normal except that pregnancy-induced mortality is seen

cardiovascular system

cardiovascular system phenotype ( J:88432 )

N	• deletion had little affect on cardiomyocyte proliferation or viability • mutants have a normal heart rate, interventricular septum thickness, and left ventricular posterior wall thickness

cardiac interstitial fibrosis ( J:88432 )

• extensive fibrosis and myofibril disarray are evident by trichome staining in older mice

dilated cardiomyopathy ( J:88432 )

• mutant hearts appear normal initially and become enlarged 5 weeks after birth

• increasing workload and stress results in manifestation of a latent defect in cardiac function

decreased heart ventricle muscle contractility ( J:88432 )

• mutants have a severe contraction defect

• the percent fractional shortening and mean velocity of circumferential fiber shortening (indicators of systolic cardiac function) are severely reduced

• in isolated cardiomyocytes resting intracellular and diastolic Ca²⁺ is normal however, with increasing pacing frequencies a significant decrease in both Ca²⁺ transients and cell contraction are detected

• defects in Ca²⁺ relaxation kinetics and reduced Ca²⁺ transients would weaken the excitation-contraction coupling in response to increasing workload

decreased heart left ventricle muscle contractility ( J:88432 )

• both left ventricular end-diastolic dimensions and end-systolic dimensions are markedly increased 5 to 6 weeks after birth

muscle

dilated cardiomyopathy ( J:88432 )

• mutant hearts appear normal initially and become enlarged 5 weeks after birth

• increasing workload and stress results in manifestation of a latent defect in cardiac function

decreased heart ventricle muscle contractility ( J:88432 )

• mutants have a severe contraction defect

• the percent fractional shortening and mean velocity of circumferential fiber shortening (indicators of systolic cardiac function) are severely reduced

• defects in Ca²⁺ relaxation kinetics and reduced Ca²⁺ transients would weaken the excitation-contraction coupling in response to increasing workload

decreased heart left ventricle muscle contractility ( J:88432 )

• both left ventricular end-diastolic dimensions and end-systolic dimensions are markedly increased 5 to 6 weeks after birth

cellular

cardiac interstitial fibrosis ( J:88432 )

• extensive fibrosis and myofibril disarray are evident by trichome staining in older mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

abnormal T cell differentiation ( J:88668 )

• in T cells sorted by stage cells with the gene deleted are progressively eliminated

• splicing of CD45 is abnormal with a significant percentage of single positive and double positive T cells expressing CD45 isoforms missing exon 5

increased double-negative T cell number ( J:88668 )

• the percentage of double-negative T cells could be elevated up to 40% of the total (compared to 6% in wild-type)

• T cell development is arrested at the CD44^-CD25⁺ stage

decreased T cell number ( J:88668 )

• the absolute number of single positive T cells is greatly reduced

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

decreased double-positive T cell number ( J:88668 )

• the percentage of double-positive T cells could be decreased down to 50% of the total (compared to 85% in wild-type)

abnormal spleen white pulp morphology ( J:88668 )

• the white pulp is paler than normal

• the absolute number of single positive T cells is significantly reduced

• none of the mature T cells found in the spleen have the gene deleted

• the spleen is otherwise unaffected

immune system

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

abnormal T cell differentiation ( J:88668 )

• in T cells sorted by stage cells with the gene deleted are progressively eliminated

• splicing of CD45 is abnormal with a significant percentage of single positive and double positive T cells expressing CD45 isoforms missing exon 5

increased double-negative T cell number ( J:88668 )

• the percentage of double-negative T cells could be elevated up to 40% of the total (compared to 6% in wild-type)

• T cell development is arrested at the CD44^-CD25⁺ stage

decreased double-positive T cell number ( J:88668 )

• the percentage of double-positive T cells could be decreased down to 50% of the total (compared to 85% in wild-type)

decreased T cell number ( J:88668 )

• the absolute number of single positive T cells is greatly reduced

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

abnormal spleen white pulp morphology ( J:88668 )

• the white pulp is paler than normal

• the absolute number of single positive T cells is significantly reduced

• none of the mature T cells found in the spleen have the gene deleted

• the spleen is otherwise unaffected

endocrine/exocrine glands

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

Allelic Composition	Srsf2^tm1Xdfu/Srsf2⁺ Tg(Lck-cre)I57Jxm/?
Genetic Background	involves: 129S4/SvJae * 129X1/SvJ * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Srsf2^tm1Xdfu mutation (1 available); any Srsf2 mutation (11 available)
Tg(Lck-cre)I57Jxm mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased thymus weight ( J:88668 )

• a modest reduction in thymic weight is seen

decreased thymocyte number ( J:88668 )

• a modest reduction in the total number of thymocytes is seen

immune system

decreased thymus weight ( J:88668 )

• a modest reduction in thymic weight is seen

decreased thymocyte number ( J:88668 )

• a modest reduction in the total number of thymocytes is seen

endocrine/exocrine glands

decreased thymus weight ( J:88668 )

• a modest reduction in thymic weight is seen

decreased thymocyte number ( J:88668 )

• a modest reduction in the total number of thymocytes is seen

Allelic Composition	Srsf2^tm1Xdfu/Srsf2^tm1Xdfu Tg(Mx1-cre)1Cgn/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J * CBA/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Srsf2^tm1Xdfu mutation (1 available); any Srsf2 mutation (11 available)
Tg(Mx1-cre)1Cgn mutation (7 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

impaired hematopoiesis ( J:221404 )

abnormal hematopoietic system physiology ( J:221404 )

• leukopenia is observed in bone marrow of lethally irritated recipient mice 18 weeks post-transplantation (pIpC injection 4 weeks after transplantation)

• anemia is observed 18 weeks post-bone marrow transplantation (pIpC injection 4 weeks after transplantation)

• hemoglobin is decreased donor bone marrow post-transplantation

• impaired reconstitution of hematopoietic stem/progenitor cells in peripheral blood in competitive bone marrow transplantation assay 14 weeks post-pIpC injection

• decrease in total hematopoietic stem cell (LSK) cell number in bone marrow in competitive bone marrow transplantation assay 14 weeks post-pIpC injection

• decrease in myeloid progenitor total cell number in bone marrow in competitive bone marrow transplantation assay 14 weeks post-pIpC injection

bone marrow failure ( J:221404 )

• bone marrow aplasia is observed 14 weeks post pIpC injection

immune system

bone marrow failure ( J:221404 )

• bone marrow aplasia is observed 14 weeks post pIpC injection

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