Phenotypes associated with this allele

• Allele Symbol: Tg(WTbeta2)4Wjk
• Allele Name: transgene insertion 4, Walter Koch
• Allele ID: MGI:3037368
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Gnai2^tm1Lbi/Gnai2^tm1Lbi Tg(WTbeta2)4Wjk/0	B6.Cg-Gnai2^tm1Lbi Tg(WTbeta2)4Wjk	MGI:3799102
cx2	Gnai2^tm1Lbi/Gnai2^+ Tg(WTbeta2)4Wjk/0	B6.Cg-Gnai2^tm1Lbi Tg(WTbeta2)4Wjk	MGI:3799103
cx3	Tg(Myh6*)140Lnwd/0 Tg(WTbeta2)4Wjk/0	involves: C57BL/6 * CBA	MGI:3799201
cx4	Csrp3^tm1Crni/Csrp3^tm1Crni Tg(WTbeta2)4Wjk/0	Not Specified	MGI:3037428
tg5	Tg(WTbeta2)4Wjk/0	B6.Cg-Tg(WTbeta2)4Wjk	MGI:3799100
tg6	Tg(WTbeta2)4Wjk/0	involves: C57BL/6	MGI:3799200
tg7	Tg(WTbeta2)4Wjk/?	Not Specified	MGI:3799090

Genotype
MGI:3799102

cx1

• Allelic Composition: Gnai2^tm1Lbi/Gnai2^tm1Lbi; Tg(WTbeta2)4Wjk/0
• Genetic Background: B6.Cg-Gnai2^tm1Lbi Tg(WTbeta2)4Wjk

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:125581 )

• all pups die between 1 and 4 days after birth

embryonic lethality, incomplete penetrance ( J:125581 )

• less then half of the expected Mendelian distribution for pups of this genotype are present at birth

growth/size/body

decreased birth body size ( J:125581 )

• pups are smaller than littermate controls at birth

behavior/neurological

weakness ( J:125581 )

• pups are weaker than littermate controls at birth

cardiovascular system

dilated heart ( J:125581 )

• dilated hearts are found at necropsy of mice that die shortly after birth

Genotype
MGI:3799103

cx2

• Allelic Composition: Gnai2^tm1Lbi/Gnai2⁺; Tg(WTbeta2)4Wjk/0
• Genetic Background: B6.Cg-Gnai2^tm1Lbi Tg(WTbeta2)4Wjk

mortality/aging

premature death ( J:125581 )

• mean survival time is 223 days compared to over 600 for control mice and most mice die by 1 year of age

behavior/neurological

fatigue ( J:125581 )

• fatigue occurs in mice with age

cardiovascular system

increased heart weight ( J:125581 )

• the heart to body weight ratio is about twice that of control mice at 7 months of age

dilated heart ( J:125581 )

• heart appears dilated at necropsy

homeostasis/metabolism

cyanosis ( J:125581 )

• occurs in mucosal membranes with age

pulmonary edema ( J:125581 )

• often found at necropsy

ascites ( J:125581 )

• often found at necropsy

liver/biliary system

abnormal liver morphology ( J:125581 )

• liver congestion is often found at necropsy

respiratory system

pulmonary edema ( J:125581 )

• often found at necropsy

respiratory distress ( J:125581 )

• occurs in mice with age

growth/size/body

increased heart weight ( J:125581 )

• the heart to body weight ratio is about twice that of control mice at 7 months of age

Genotype
MGI:3799201

cx3

• Allelic Composition: Tg(Myh6*)140Lnwd/0; Tg(WTbeta2)4Wjk/0
• Genetic Background: involves: C57BL/6 * CBA

mortality/aging

premature death ( J:134706 )

• mice have increased mortality with only 50% survival at 8 months and less than 10% survival at 12 months

cardiovascular system

increased heart weight ( J:134706 )

• the heart weight to body weight ratio is 67% greater compared to controls

cardiac hypertrophy ( J:134706 )

• fibrosis and cellular disarray are severe in hearts of 8 month old mice

• hearts show dilated ventricular and atrial chambers with thin, fibrotic ventricular walls

abnormal cardiac muscle contractility ( J:134706 )

• mice have increased fractional shortening at 4 months of age but not 8 months of age

• by 8 months of age, fractional shortening has become significantly impaired to two-thirds of control

congestive heart failure ( J:134706 )

• some mice show the signs of congestive heart failure including pulmonary and hepatic enlargement, atrial and ventricular dilation, intraperitoneal edema, and massively enlarged hearts

muscle

abnormal cardiac muscle contractility ( J:134706 )

• mice have increased fractional shortening at 4 months of age but not 8 months of age

• by 8 months of age, fractional shortening has become significantly impaired to two-thirds of control

growth/size/body

increased heart weight ( J:134706 )

• the heart weight to body weight ratio is 67% greater compared to controls

cardiac hypertrophy ( J:134706 )

• fibrosis and cellular disarray are severe in hearts of 8 month old mice

• hearts show dilated ventricular and atrial chambers with thin, fibrotic ventricular walls

Genotype
MGI:3037428

cx4

• Allelic Composition: Csrp3^tm1Crni/Csrp3^tm1Crni; Tg(WTbeta2)4Wjk/0
• Genetic Background: Not Specified

cardiovascular system

dilated heart left ventricle ( J:48062 )

dilated cardiomyopathy ( J:48062 )

• the overexpression of ADRB2 did not influence the dilated cardiomyopathy observed in Csrp3^tm1Crni homozygotes

decreased heart ventricle muscle contractility ( J:48062 )

muscle

dilated cardiomyopathy ( J:48062 )

• the overexpression of ADRB2 did not influence the dilated cardiomyopathy observed in Csrp3^tm1Crni homozygotes

decreased heart ventricle muscle contractility ( J:48062 )

Genotype
MGI:3799100

tg5

• Allelic Composition: Tg(WTbeta2)4Wjk/0
• Genetic Background: B6.Cg-Tg(WTbeta2)4Wjk

mortality/aging

premature death ( J:125581 )

• mean survival time is 307 days compared to over 600 for control mice and most mice die by 1 year of age

behavior/neurological

fatigue ( J:125581 )

• fatigue occurs in mice when approaching 1 year of age

respiratory system

respiratory distress ( J:125581 )

• occurs in mice when approaching 1 year of age

homeostasis/metabolism

cyanosis ( J:125581 )

• occurs in mucosal membranes as mice approach 1 year of age

Genotype
MGI:3799200

tg6

• Allelic Composition: Tg(WTbeta2)4Wjk/0
• Genetic Background: involves: C57BL/6

mortality/aging

premature death ( J:134706 )

• mice show increased mortality by one year compared to controls

cardiovascular system

cardiac fibrosis ( J:134706 )

• hearts of 8 month old mice show area of significant fibrosis

abnormal cardiac muscle contractility ( J:134706 )

• mice have increased fractional shortening at 4 months of age but not 8 months of age

• by 12 months of age, fractional shortening has become significantly impaired

homeostasis/metabolism

increased aerobic running capacity ( J:134706 )

• mice have increased exercise tolerance on a treadmill compared to controls at 6 months of age

muscle

abnormal cardiac muscle contractility ( J:134706 )

• mice have increased fractional shortening at 4 months of age but not 8 months of age

• by 12 months of age, fractional shortening has become significantly impaired

Genotype
MGI:3799090

tg7

• Allelic Composition: Tg(WTbeta2)4Wjk/?
• Genetic Background: Not Specified

mortality/aging

premature death ( J:128583 )

• loss of mice starts at 8.5 months with a cumulative mortality of 62% by 12 months and 81% by 15 months

growth/size/body

heart left ventricle hypertrophy ( J:128583 )

• left ventricle mass is increased indicating left ventricle hypertrophy

decreased susceptibility to weight gain ( J:128583 )

• body weight is normal at 4-9 months of age, however mice that die or are sacrificed at 15 months of age have a lower body weight, with wild-type mice showing a 30% increase in body weight compared to mutants

cardiovascular system

pulmonary vascular congestion ( J:128583 )

• lungs are congested with thickening of alveolar walls and increase in cellular components

abnormal myocardial fiber morphology ( J:128583 )

• myocytes frequently exhibit polymorphism of nuclei

increased myocardial fiber size ( J:128583 )

• hearts show dropout of myocytes and hypertrophy of remaining myocytes

• cross-sectional area of myocytes is 3x greater

myocardial fiber degeneration ( J:128583 )

• death of cardiac myocytes in areas of fibrosis

abnormal heart left ventricle morphology ( J:128583 )

• 8-fold increase in collagen content in left ventricles

heart left ventricle hypertrophy ( J:128583 )

• left ventricle mass is increased indicating left ventricle hypertrophy

dilated heart left ventricle ( J:128583 )

• starting at 9 months of age, mice develop progressive left ventricle dilatation

cardiac interstitial fibrosis ( J:128583 )

• hearts show widespread interstitial fibrosis

• interstitial fibrosis is most severe in left ventricular free wall, the apex, the atrio-ventricular junction and perivascular regions

decreased cardiac muscle contractility ( J:128583 )

• reduction in fractional shortening after 9 months of age

• wall thickening index of both anterior and posterior walls is lower at 12-15 months, suggesting weakened muscle contraction

increased cardiac muscle contractility ( J:17862 )

• the isometric tension of isolated left atria is three times that of controls while the isometric tension of the right atria is about 75% higher than in wild-type controls

• these parameters at baseline are equal to those observed in control mice maximally stimulated with isoproterenol

increased heart left ventricle muscle contractility ( J:17862 )

• the baseline left ventricle dP/dT-max is 70% higher than in controls

• administration of isoproterenol fails to increase the dP/dTmax as it does in wild-type controls

• this parameter at baseline is equal to those observed in control mice maximally stimulated with isoproterenol

abnormal cardiac muscle relaxation ( J:17862 )

• left ventricle relaxation is enhanced compared to controls with a mean drop in pressure of 5328 mm Hg versus 3313 mm Hg

abnormal heart echocardiography feature ( J:128583 )

• echocardiography shows increases in internal and external left ventricle dimensions of diastole, indicating left ventricle dilatation

increased heart rate ( J:17862 , J:128583 )

• the mean heart rate is about 425 bpm compared to 340 bpm in control mice (J:17862)

• there is about a 50% increase in the heart rate of isolated right atria (J:17862)

ventricular premature beat ( J:128583 )

• ectopic beats mainly occur as singles but occasionally as salvos

abnormal heart electrocardiography waveform feature ( J:128583 )

• mice exhibit altered configuration of standard lead II ECG waves and development of ectopic beats

• majority of mice exhibit a large Q wave

shortened ST segment ( J:128583 )

• majority of mice exhibit suppressed S-T segment

cardiomyopathy ( J:128583 )

• develops with age

congestive heart failure ( J:128583 )

• 17 of 21 mice die of heart failure

homeostasis/metabolism

abnormal atrial thrombosis ( J:128583 )

• higher incidence of chronic thrombus in the left atrium

pleural effusion ( J:128583 )

• higher incidence of pleural effusion

increased collagen level ( J:128583 )

• 8-fold increase in collagen content in left ventricles

muscle

abnormal myocardial fiber morphology ( J:128583 )

• myocytes frequently exhibit polymorphism of nuclei

increased myocardial fiber size ( J:128583 )

• hearts show dropout of myocytes and hypertrophy of remaining myocytes

• cross-sectional area of myocytes is 3x greater

myocardial fiber degeneration ( J:128583 )

• death of cardiac myocytes in areas of fibrosis

heart left ventricle hypertrophy ( J:128583 )

• left ventricle mass is increased indicating left ventricle hypertrophy

decreased cardiac muscle contractility ( J:128583 )

• reduction in fractional shortening after 9 months of age

• wall thickening index of both anterior and posterior walls is lower at 12-15 months, suggesting weakened muscle contraction

increased cardiac muscle contractility ( J:17862 )

• the isometric tension of isolated left atria is three times that of controls while the isometric tension of the right atria is about 75% higher than in wild-type controls

• these parameters at baseline are equal to those observed in control mice maximally stimulated with isoproterenol

increased heart left ventricle muscle contractility ( J:17862 )

• the baseline left ventricle dP/dT-max is 70% higher than in controls

• administration of isoproterenol fails to increase the dP/dTmax as it does in wild-type controls

• this parameter at baseline is equal to those observed in control mice maximally stimulated with isoproterenol

abnormal cardiac muscle relaxation ( J:17862 )

• left ventricle relaxation is enhanced compared to controls with a mean drop in pressure of 5328 mm Hg versus 3313 mm Hg

cardiomyopathy ( J:128583 )

• develops with age

respiratory system

pulmonary vascular congestion ( J:128583 )

• lungs are congested with thickening of alveolar walls and increase in cellular components

pleural effusion ( J:128583 )

• higher incidence of pleural effusion

cellular

cardiac interstitial fibrosis ( J:128583 )

• hearts show widespread interstitial fibrosis

• interstitial fibrosis is most severe in left ventricular free wall, the apex, the atrio-ventricular junction and perivascular regions