All Phenotypes Tg(CD19-cre/ERT2)1Cgn MGI Mouse

About Help FAQ

Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(CD19-cre/ERT2)1Cgn
transgene insertion 1, University of Cologne
MGI:3037896

Summary

7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Igh^tm5Cgn/Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn/0	C.Cg-Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn	MGI:3037901
cn2	Traf3ip2^tm1Lix/Traf3ip2^tm1.1Lix Tg(CD19-cre/ERT2)1Cgn/0	involves: 129/Sv * BALB/c	MGI:3511174
cn3	Igh^tm1.2Rsky/Igh^tm1.2Rsky Tg(CD19-cre/ERT2)1Cgn/?	involves: BALB/c	MGI:3760857
cn4	Ppp2r3c^tm1Imku/Ppp2r3c^tm1Imku Tg(CD19-cre/ERT2)1Cgn/?	involves: C57BL/6	MGI:3759748
cn5	Nbn^tm1Nus/Nbn^tm2Nus Tg(CD19-cre/ERT2)1Cgn/0	Not Specified	MGI:3573788
cn6	Cyld^tm1Awai/Cyld^tm1Awai Tg(CD19-cre/ERT2)1Cgn/?	Not Specified	MGI:3761629
cn7	Ikzf1^tm3Kge/Ikzf1^tm3Kge Tg(CD19-cre/ERT2)1Cgn/?	Not Specified	MGI:5698880

Allelic Composition	Igh^tm5Cgn/Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn/0
Genetic Background	C.Cg-Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm5Cgn mutation (0 available); any Igh mutation (44 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell differentiation ( J:88472 )

• B cell development is almost exclusively restricted to the generation of B-1 cells

increased pro-B cell number ( J:88472 )

• mutant mice display a higher percentage of pro-B cells (CD19⁺CD43⁺) in the bone marrow relative to wild-type controls

increased B cell number ( J:88472 )

• mutant mice display an increase in the number of B cells in spleen (4-fold), lymph nodes (2-fold) and peritoneal cavity (2-fold) relative to age-matched wild-type controls

abnormal mature B cell morphology ( J:88472 )

• >90% of peripheral mutant B cells exhibit a B-1 cell phenotype (CD24^intCD38^hi)

• no follicular and marginal zone B cells are identified, as most splenic B cells are CD5^intCD21^loCD23^lo

absent follicular B cells ( J:88472 )

absent marginal zone B cells ( J:88472 )

immune system

abnormal B cell differentiation ( J:88472 )

• B cell development is almost exclusively restricted to the generation of B-1 cells

increased pro-B cell number ( J:88472 )

• mutant mice display a higher percentage of pro-B cells (CD19⁺CD43⁺) in the bone marrow relative to wild-type controls

increased B cell number ( J:88472 )

• mutant mice display an increase in the number of B cells in spleen (4-fold), lymph nodes (2-fold) and peritoneal cavity (2-fold) relative to age-matched wild-type controls

abnormal mature B cell morphology ( J:88472 )

• >90% of peripheral mutant B cells exhibit a B-1 cell phenotype (CD24^intCD38^hi)

• no follicular and marginal zone B cells are identified, as most splenic B cells are CD5^intCD21^loCD23^lo

absent follicular B cells ( J:88472 )

absent marginal zone B cells ( J:88472 )

abnormal Peyer's patch germinal center morphology ( J:88472 )

• non-immunized mutant mice exhibit occasional spontaneous germinal center formation in Peyer's patches

Allelic Composition	Traf3ip2^tm1Lix/Traf3ip2^tm1.1Lix Tg(CD19-cre/ERT2)1Cgn/0
Genetic Background	involves: 129/Sv * BALB/c

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)
Traf3ip2^tm1.1Lix mutation (0 available); any Traf3ip2 mutation (41 available)
Traf3ip2^tm1Lix mutation (0 available); any Traf3ip2 mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

enlarged spleen ( J:93923 )

• this is less severe than in Act1 null mice

increased dendritic cell number ( J:93923 )

• the number of dendritic cells is increased in the spleen, this is less severe than in Act1 null mice

increased B cell number ( J:93923 )

• the total splenic B cell population is increased however the proportions of different B cell stages are the same as in wild-type littermates

• this is less severe than in Act1 null mice

increased immunoglobulin level ( J:93923 )

• this is less severe than in Act1 null mice

immune system

enlarged spleen ( J:93923 )

• this is less severe than in Act1 null mice

increased dendritic cell number ( J:93923 )

• the number of dendritic cells is increased in the spleen, this is less severe than in Act1 null mice

increased B cell number ( J:93923 )

• the total splenic B cell population is increased however the proportions of different B cell stages are the same as in wild-type littermates

• this is less severe than in Act1 null mice

increased immunoglobulin level ( J:93923 )

• this is less severe than in Act1 null mice

enlarged lymph nodes ( J:93923 )

• this is less severe than in Act1 null mice

growth/size/body

enlarged spleen ( J:93923 )

• this is less severe than in Act1 null mice

Allelic Composition	Igh^tm1.2Rsky/Igh^tm1.2Rsky Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	involves: BALB/c

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm1.2Rsky mutation (0 available); any Igh mutation (44 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal class switch recombination ( J:125719 )

• upon tamoxifen treatment, B cells switch immunoglobulin expression from IgG1 to IgM

abnormal B cell activation ( J:125719 )

• enhanced Ca²⁺ mobilization upon activation of B cells

hematopoietic system

abnormal class switch recombination ( J:125719 )

• upon tamoxifen treatment, B cells switch immunoglobulin expression from IgG1 to IgM

abnormal B cell activation ( J:125719 )

• enhanced Ca²⁺ mobilization upon activation of B cells

Allelic Composition	Ppp2r3c^tm1Imku/Ppp2r3c^tm1Imku Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppp2r3c^tm1Imku mutation (0 available); any Ppp2r3c mutation (43 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased transitional stage B cell number ( J:100706 )

• the number of transitional-1 (IgM^highIgD^low) B cells is increased (5.38% compared to 3.5 % in heterozygotes)

increased pro-B cell number ( J:100706 )

• the number of pro-B cells (B220^lowCD43+) is high relative to in heterozygotes

decreased B cell number ( J:100706 )

• the number of splenic B cells is reduced to 60% of the number in heterozygotes

• mice have half the number of B220+ B cells as do heterozygous mice

• IgM+IgD+ recirculating B cells are decreased in number (to 7.04% from 13.4% in heterozygous mice)

• the proportion of B cell in the spleen is decreased (35.3% compared to 48.3% in heterozygotes)

• the number of IgD+ B cells in the extrafollicular regions of the spleen is decreased

decreased immature B cell number ( J:100706 )

• the number of pre-B cells and immature B cells (B220^lowCD43-) is decreased from 31% to 22.7% of control B cells

decreased transitional stage B cell number ( J:100706 )

• the number of transitional-2 (IgM^highIgD^high) B cells is decreased (7.08% compared to 9.88% in heterozygotes)

decreased mature B cell number ( J:100706 )

• the number of mature B cells (B220^highCD43-) is 2.72% (50% of the number in heterozygous mice)

• the number of IgM^lowIgD^high mature B cells is decreased (20.8% compared to 31.3% in heterozygotes)

• the proportion of mature B cells is decreased in the auxillary lymph nodes (9.33% compared to 16.4% in heterozyotes) and peripheral blood (18.1% compared to 51.3% in heterozygotes)

decreased B-1a cell number ( J:100706 )

• the number of B-1a cells is decreased (10.5% compared to 17.2% in heterozygotes)

decreased follicular B cell number ( J:100706 )

• the number of mature follicle B cells is decreased (27.1% compared to 40% in heterozygotes)

decreased pre-B cell number ( J:100706 )

increased T cell number ( J:100706 )

• the proportion of mature T cells in the spleen is increased (49.9% compared to 39% in heterozygotes)

small spleen ( J:100706 )

decreased spleen germinal center size ( J:100706 )

• after immunization with sheep red blood cells initiates small germinal centers relative to in heterozygotes

abnormal B cell activation ( J:100706 )

• following B cell receptor stimulation, B cells exhibit increased apoptosis (33.8% compared to 15.3% in heterozygotes) that is caspase-3 independent, increased mitochondrial membrane depolarization (64% compared to 43% in heterozygotes), and more single and double-strand DNA breaks (in 32.2% of cells compared to 21.1% of cells from heterozygotes)

• however, the level of spontaneous apoptosis and response to LPS stimulation are normal

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

abnormal humoral immune response ( J:100706 )

• IgG response to TNP-Ficoll and TNP-keyhole limpet hemocyanin is slightly decreased at 14 days after immunization

• however, IgM response to TNP-Ficoll and TNP-keyhole limpet hemocyanin is normal

hematopoietic system

increased transitional stage B cell number ( J:100706 )

• the number of transitional-1 (IgM^highIgD^low) B cells is increased (5.38% compared to 3.5 % in heterozygotes)

increased pro-B cell number ( J:100706 )

• the number of pro-B cells (B220^lowCD43+) is high relative to in heterozygotes

decreased B cell number ( J:100706 )

• the number of splenic B cells is reduced to 60% of the number in heterozygotes

• mice have half the number of B220+ B cells as do heterozygous mice

• IgM+IgD+ recirculating B cells are decreased in number (to 7.04% from 13.4% in heterozygous mice)

• the proportion of B cell in the spleen is decreased (35.3% compared to 48.3% in heterozygotes)

• the number of IgD+ B cells in the extrafollicular regions of the spleen is decreased

decreased immature B cell number ( J:100706 )

• the number of pre-B cells and immature B cells (B220^lowCD43-) is decreased from 31% to 22.7% of control B cells

decreased transitional stage B cell number ( J:100706 )

• the number of transitional-2 (IgM^highIgD^high) B cells is decreased (7.08% compared to 9.88% in heterozygotes)

decreased mature B cell number ( J:100706 )

• the number of mature B cells (B220^highCD43-) is 2.72% (50% of the number in heterozygous mice)

• the number of IgM^lowIgD^high mature B cells is decreased (20.8% compared to 31.3% in heterozygotes)

• the proportion of mature B cells is decreased in the auxillary lymph nodes (9.33% compared to 16.4% in heterozyotes) and peripheral blood (18.1% compared to 51.3% in heterozygotes)

decreased B-1a cell number ( J:100706 )

• the number of B-1a cells is decreased (10.5% compared to 17.2% in heterozygotes)

decreased follicular B cell number ( J:100706 )

• the number of mature follicle B cells is decreased (27.1% compared to 40% in heterozygotes)

decreased pre-B cell number ( J:100706 )

increased T cell number ( J:100706 )

• the proportion of mature T cells in the spleen is increased (49.9% compared to 39% in heterozygotes)

small spleen ( J:100706 )

decreased spleen germinal center size ( J:100706 )

• after immunization with sheep red blood cells initiates small germinal centers relative to in heterozygotes

abnormal B cell activation ( J:100706 )

• however, the level of spontaneous apoptosis and response to LPS stimulation are normal

• following B cell receptor stimulation, B cells exhibit increased apoptosis (33.8% compared to 15.3% in heterozygotes) that is caspase-3 independent, increased mitochondrial membrane depolarization (64% compared to 43% in heterozygotes), and more single and double-strand DNA breaks (in 32.2% of cells compared to 21.1% of cells from heterozygotes)

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

cellular

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

Allelic Composition	Nbn^tm1Nus/Nbn^tm2Nus Tg(CD19-cre/ERT2)1Cgn/0
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbn^tm1Nus mutation (0 available); any Nbn mutation (59 available)
Nbn^tm2Nus mutation (0 available); any Nbn mutation (59 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell physiology ( J:96102 )

• recombined B lymphocytes show increased spontaneous DNA damage, chromosomal aberrations including tetraploidy, and Ig class-switch recombination is diminished

decreased B cell proliferation ( J:96102 )

• after 72 hours in culture only 39% of mutant B cells had completed 3 or more cell divisions, cells accumulated in the G₂/M phase of the cell cycle, and relatively increased cell death is seen

decreased IgG level ( J:96102 )

• IgG, IgG1, IgG2b and IgG3 levels are reduced

• IgM levels are normal

decreased IgG1 level ( J:96102 )

decreased IgG2a level ( J:96102 )

decreased IgG2b level ( J:96102 )

decreased IgG3 level ( J:96102 )

hematopoietic system

abnormal B cell physiology ( J:96102 )

• recombined B lymphocytes show increased spontaneous DNA damage, chromosomal aberrations including tetraploidy, and Ig class-switch recombination is diminished

decreased B cell proliferation ( J:96102 )

• after 72 hours in culture only 39% of mutant B cells had completed 3 or more cell divisions, cells accumulated in the G₂/M phase of the cell cycle, and relatively increased cell death is seen

decreased IgG level ( J:96102 )

• IgG, IgG1, IgG2b and IgG3 levels are reduced

• IgM levels are normal

decreased IgG1 level ( J:96102 )

decreased IgG2a level ( J:96102 )

decreased IgG2b level ( J:96102 )

decreased IgG3 level ( J:96102 )

cellular

decreased B cell proliferation ( J:96102 )

• after 72 hours in culture only 39% of mutant B cells had completed 3 or more cell divisions, cells accumulated in the G₂/M phase of the cell cycle, and relatively increased cell death is seen

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Nijmegen breakage syndrome		DOID:7400	OMIM:251260	J:96102

Allelic Composition	Cyld^tm1Awai/Cyld^tm1Awai Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cyld^tm1Awai mutation (0 available); any Cyld mutation (44 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

enlarged spleen ( J:126113 )

decreased immature B cell number ( J:126113 )

increased B cell number ( J:126113 )

• 100.8+/-9.5x10⁶ compared to 46.7+/-4.8x10⁶ B cells in the spleen of wild mice

abnormal mature B cell morphology ( J:126113 )

• the ratio of B-1 to B-2 B cells is inverted compared to in wild-type mice in the peritoneal cavity

decreased B-1 B cell number ( J:126113 )

• the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)

enlarged Peyer's patches ( J:126113 )

enlarged lymph nodes ( J:126113 )

hematopoietic system

enlarged spleen ( J:126113 )

decreased immature B cell number ( J:126113 )

increased B cell number ( J:126113 )

• 100.8+/-9.5x10⁶ compared to 46.7+/-4.8x10⁶ B cells in the spleen of wild mice

abnormal mature B cell morphology ( J:126113 )

• the ratio of B-1 to B-2 B cells is inverted compared to in wild-type mice in the peritoneal cavity

decreased B-1 B cell number ( J:126113 )

• the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)

growth/size/body

enlarged spleen ( J:126113 )

Allelic Composition	Ikzf1^tm3Kge/Ikzf1^tm3Kge Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ikzf1^tm3Kge mutation (0 available); any Ikzf1 mutation (30 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell apoptosis ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cell apoptosis increases

decreased pre-B cell number ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cells rapidly decline in number

absent pre-B cells ( J:209927 )

• circulating pre-B cells are not found

cellular

abnormal B cell apoptosis ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cell apoptosis increases

immune system

abnormal B cell apoptosis ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cell apoptosis increases

decreased pre-B cell number ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cells rapidly decline in number

absent pre-B cells ( J:209927 )

• circulating pre-B cells are not found

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24