All Phenotypes Tg(CD19-cre/ERT2)1Cgn MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(CD19-cre/ERT2)1Cgn
transgene insertion 1, University of Cologne
MGI:3037896

Summary

7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Igh^tm5Cgn/Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn/0	C.Cg-Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn	MGI:3037901
cn2	Traf3ip2^tm1Lix/Traf3ip2^tm1.1Lix Tg(CD19-cre/ERT2)1Cgn/0	involves: 129/Sv * BALB/c	MGI:3511174
cn3	Igh^tm1.2Rsky/Igh^tm1.2Rsky Tg(CD19-cre/ERT2)1Cgn/?	involves: BALB/c	MGI:3760857
cn4	Ppp2r3c^tm1Imku/Ppp2r3c^tm1Imku Tg(CD19-cre/ERT2)1Cgn/?	involves: C57BL/6	MGI:3759748
cn5	Nbn^tm1Nus/Nbn^tm2Nus Tg(CD19-cre/ERT2)1Cgn/0	Not Specified	MGI:3573788
cn6	Cyld^tm1Awai/Cyld^tm1Awai Tg(CD19-cre/ERT2)1Cgn/?	Not Specified	MGI:3761629
cn7	Ikzf1^tm3Kge/Ikzf1^tm3Kge Tg(CD19-cre/ERT2)1Cgn/?	Not Specified	MGI:5698880

Allelic Composition	Igh^tm5Cgn/Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn/0
Genetic Background	C.Cg-Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm5Cgn mutation (0 available); any Igh mutation (44 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell differentiation ( J:88472 )

• B cell development is almost exclusively restricted to the generation of B-1 cells

increased pro-B cell number ( J:88472 )

• mutant mice display a higher percentage of pro-B cells (CD19⁺CD43⁺) in the bone marrow relative to wild-type controls

increased B cell number ( J:88472 )

• mutant mice display an increase in the number of B cells in spleen (4-fold), lymph nodes (2-fold) and peritoneal cavity (2-fold) relative to age-matched wild-type controls

abnormal mature B cell morphology ( J:88472 )

• >90% of peripheral mutant B cells exhibit a B-1 cell phenotype (CD24^intCD38^hi)

• no follicular and marginal zone B cells are identified, as most splenic B cells are CD5^intCD21^loCD23^lo

absent follicular B cells ( J:88472 )

absent marginal zone B cells ( J:88472 )

immune system

abnormal B cell differentiation ( J:88472 )

• B cell development is almost exclusively restricted to the generation of B-1 cells

increased pro-B cell number ( J:88472 )

• mutant mice display a higher percentage of pro-B cells (CD19⁺CD43⁺) in the bone marrow relative to wild-type controls

increased B cell number ( J:88472 )

• mutant mice display an increase in the number of B cells in spleen (4-fold), lymph nodes (2-fold) and peritoneal cavity (2-fold) relative to age-matched wild-type controls

abnormal mature B cell morphology ( J:88472 )

• >90% of peripheral mutant B cells exhibit a B-1 cell phenotype (CD24^intCD38^hi)

• no follicular and marginal zone B cells are identified, as most splenic B cells are CD5^intCD21^loCD23^lo

absent follicular B cells ( J:88472 )

absent marginal zone B cells ( J:88472 )

abnormal Peyer's patch germinal center morphology ( J:88472 )

• non-immunized mutant mice exhibit occasional spontaneous germinal center formation in Peyer's patches

Allelic Composition	Traf3ip2^tm1Lix/Traf3ip2^tm1.1Lix Tg(CD19-cre/ERT2)1Cgn/0
Genetic Background	involves: 129/Sv * BALB/c

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)
Traf3ip2^tm1.1Lix mutation (0 available); any Traf3ip2 mutation (41 available)
Traf3ip2^tm1Lix mutation (0 available); any Traf3ip2 mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

enlarged spleen ( J:93923 )

• this is less severe than in Act1 null mice

increased dendritic cell number ( J:93923 )

• the number of dendritic cells is increased in the spleen, this is less severe than in Act1 null mice

increased B cell number ( J:93923 )

• the total splenic B cell population is increased however the proportions of different B cell stages are the same as in wild-type littermates

• this is less severe than in Act1 null mice

increased immunoglobulin level ( J:93923 )

• this is less severe than in Act1 null mice

immune system

enlarged spleen ( J:93923 )

• this is less severe than in Act1 null mice

increased dendritic cell number ( J:93923 )

• the number of dendritic cells is increased in the spleen, this is less severe than in Act1 null mice

increased B cell number ( J:93923 )

• the total splenic B cell population is increased however the proportions of different B cell stages are the same as in wild-type littermates

• this is less severe than in Act1 null mice

increased immunoglobulin level ( J:93923 )

• this is less severe than in Act1 null mice

enlarged lymph nodes ( J:93923 )

• this is less severe than in Act1 null mice

growth/size/body

enlarged spleen ( J:93923 )

• this is less severe than in Act1 null mice

Allelic Composition	Igh^tm1.2Rsky/Igh^tm1.2Rsky Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	involves: BALB/c

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm1.2Rsky mutation (0 available); any Igh mutation (44 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal class switch recombination ( J:125719 )

• upon tamoxifen treatment, B cells switch immunoglobulin expression from IgG1 to IgM

abnormal B cell activation ( J:125719 )

• enhanced Ca²⁺ mobilization upon activation of B cells

hematopoietic system

abnormal class switch recombination ( J:125719 )

• upon tamoxifen treatment, B cells switch immunoglobulin expression from IgG1 to IgM

abnormal B cell activation ( J:125719 )

• enhanced Ca²⁺ mobilization upon activation of B cells

Allelic Composition	Ppp2r3c^tm1Imku/Ppp2r3c^tm1Imku Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppp2r3c^tm1Imku mutation (0 available); any Ppp2r3c mutation (43 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased transitional stage B cell number ( J:100706 )

• the number of transitional-1 (IgM^highIgD^low) B cells is increased (5.38% compared to 3.5 % in heterozygotes)

increased pro-B cell number ( J:100706 )

• the number of pro-B cells (B220^lowCD43+) is high relative to in heterozygotes

decreased B cell number ( J:100706 )

• the number of splenic B cells is reduced to 60% of the number in heterozygotes

• mice have half the number of B220+ B cells as do heterozygous mice

• IgM+IgD+ recirculating B cells are decreased in number (to 7.04% from 13.4% in heterozygous mice)

• the proportion of B cell in the spleen is decreased (35.3% compared to 48.3% in heterozygotes)

• the number of IgD+ B cells in the extrafollicular regions of the spleen is decreased

decreased immature B cell number ( J:100706 )

• the number of pre-B cells and immature B cells (B220^lowCD43-) is decreased from 31% to 22.7% of control B cells

decreased transitional stage B cell number ( J:100706 )

• the number of transitional-2 (IgM^highIgD^high) B cells is decreased (7.08% compared to 9.88% in heterozygotes)

decreased mature B cell number ( J:100706 )

• the number of mature B cells (B220^highCD43-) is 2.72% (50% of the number in heterozygous mice)

• the number of IgM^lowIgD^high mature B cells is decreased (20.8% compared to 31.3% in heterozygotes)

• the proportion of mature B cells is decreased in the auxillary lymph nodes (9.33% compared to 16.4% in heterozyotes) and peripheral blood (18.1% compared to 51.3% in heterozygotes)

decreased B-1a cell number ( J:100706 )

• the number of B-1a cells is decreased (10.5% compared to 17.2% in heterozygotes)

decreased follicular B cell number ( J:100706 )

• the number of mature follicle B cells is decreased (27.1% compared to 40% in heterozygotes)

decreased pre-B cell number ( J:100706 )

increased T cell number ( J:100706 )

• the proportion of mature T cells in the spleen is increased (49.9% compared to 39% in heterozygotes)

small spleen ( J:100706 )

decreased spleen germinal center size ( J:100706 )

• after immunization with sheep red blood cells initiates small germinal centers relative to in heterozygotes

abnormal B cell activation ( J:100706 )

• following B cell receptor stimulation, B cells exhibit increased apoptosis (33.8% compared to 15.3% in heterozygotes) that is caspase-3 independent, increased mitochondrial membrane depolarization (64% compared to 43% in heterozygotes), and more single and double-strand DNA breaks (in 32.2% of cells compared to 21.1% of cells from heterozygotes)

• however, the level of spontaneous apoptosis and response to LPS stimulation are normal

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

abnormal humoral immune response ( J:100706 )

• IgG response to TNP-Ficoll and TNP-keyhole limpet hemocyanin is slightly decreased at 14 days after immunization

• however, IgM response to TNP-Ficoll and TNP-keyhole limpet hemocyanin is normal

hematopoietic system

increased transitional stage B cell number ( J:100706 )

• the number of transitional-1 (IgM^highIgD^low) B cells is increased (5.38% compared to 3.5 % in heterozygotes)

increased pro-B cell number ( J:100706 )

• the number of pro-B cells (B220^lowCD43+) is high relative to in heterozygotes

decreased B cell number ( J:100706 )

• the number of splenic B cells is reduced to 60% of the number in heterozygotes

• mice have half the number of B220+ B cells as do heterozygous mice

• IgM+IgD+ recirculating B cells are decreased in number (to 7.04% from 13.4% in heterozygous mice)

• the proportion of B cell in the spleen is decreased (35.3% compared to 48.3% in heterozygotes)

• the number of IgD+ B cells in the extrafollicular regions of the spleen is decreased

decreased immature B cell number ( J:100706 )

• the number of pre-B cells and immature B cells (B220^lowCD43-) is decreased from 31% to 22.7% of control B cells

decreased transitional stage B cell number ( J:100706 )

• the number of transitional-2 (IgM^highIgD^high) B cells is decreased (7.08% compared to 9.88% in heterozygotes)

decreased mature B cell number ( J:100706 )

• the number of mature B cells (B220^highCD43-) is 2.72% (50% of the number in heterozygous mice)

• the number of IgM^lowIgD^high mature B cells is decreased (20.8% compared to 31.3% in heterozygotes)

• the proportion of mature B cells is decreased in the auxillary lymph nodes (9.33% compared to 16.4% in heterozyotes) and peripheral blood (18.1% compared to 51.3% in heterozygotes)

decreased B-1a cell number ( J:100706 )

• the number of B-1a cells is decreased (10.5% compared to 17.2% in heterozygotes)

decreased follicular B cell number ( J:100706 )

• the number of mature follicle B cells is decreased (27.1% compared to 40% in heterozygotes)

decreased pre-B cell number ( J:100706 )

increased T cell number ( J:100706 )

• the proportion of mature T cells in the spleen is increased (49.9% compared to 39% in heterozygotes)

small spleen ( J:100706 )

decreased spleen germinal center size ( J:100706 )

• after immunization with sheep red blood cells initiates small germinal centers relative to in heterozygotes

abnormal B cell activation ( J:100706 )

• following B cell receptor stimulation, B cells exhibit increased apoptosis (33.8% compared to 15.3% in heterozygotes) that is caspase-3 independent, increased mitochondrial membrane depolarization (64% compared to 43% in heterozygotes), and more single and double-strand DNA breaks (in 32.2% of cells compared to 21.1% of cells from heterozygotes)

• however, the level of spontaneous apoptosis and response to LPS stimulation are normal

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

cellular

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

Allelic Composition	Nbn^tm1Nus/Nbn^tm2Nus Tg(CD19-cre/ERT2)1Cgn/0
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbn^tm1Nus mutation (0 available); any Nbn mutation (59 available)
Nbn^tm2Nus mutation (0 available); any Nbn mutation (59 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell physiology ( J:96102 )

• recombined B lymphocytes show increased spontaneous DNA damage, chromosomal aberrations including tetraploidy, and Ig class-switch recombination is diminished

decreased B cell proliferation ( J:96102 )

• after 72 hours in culture only 39% of mutant B cells had completed 3 or more cell divisions, cells accumulated in the G₂/M phase of the cell cycle, and relatively increased cell death is seen

decreased IgG level ( J:96102 )

• IgG, IgG1, IgG2b and IgG3 levels are reduced

• IgM levels are normal

decreased IgG1 level ( J:96102 )

decreased IgG2a level ( J:96102 )

decreased IgG2b level ( J:96102 )

decreased IgG3 level ( J:96102 )

hematopoietic system

abnormal B cell physiology ( J:96102 )

• recombined B lymphocytes show increased spontaneous DNA damage, chromosomal aberrations including tetraploidy, and Ig class-switch recombination is diminished

decreased B cell proliferation ( J:96102 )

• after 72 hours in culture only 39% of mutant B cells had completed 3 or more cell divisions, cells accumulated in the G₂/M phase of the cell cycle, and relatively increased cell death is seen

decreased IgG level ( J:96102 )

• IgG, IgG1, IgG2b and IgG3 levels are reduced

• IgM levels are normal

decreased IgG1 level ( J:96102 )

decreased IgG2a level ( J:96102 )

decreased IgG2b level ( J:96102 )

decreased IgG3 level ( J:96102 )

cellular

decreased B cell proliferation ( J:96102 )

• after 72 hours in culture only 39% of mutant B cells had completed 3 or more cell divisions, cells accumulated in the G₂/M phase of the cell cycle, and relatively increased cell death is seen

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Nijmegen breakage syndrome		DOID:7400	OMIM:251260	J:96102

Allelic Composition	Cyld^tm1Awai/Cyld^tm1Awai Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cyld^tm1Awai mutation (0 available); any Cyld mutation (44 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

enlarged spleen ( J:126113 )

decreased immature B cell number ( J:126113 )

increased B cell number ( J:126113 )

• 100.8+/-9.5x10⁶ compared to 46.7+/-4.8x10⁶ B cells in the spleen of wild mice

abnormal mature B cell morphology ( J:126113 )

• the ratio of B-1 to B-2 B cells is inverted compared to in wild-type mice in the peritoneal cavity

decreased B-1 B cell number ( J:126113 )

• the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)

enlarged Peyer's patches ( J:126113 )

enlarged lymph nodes ( J:126113 )

hematopoietic system

enlarged spleen ( J:126113 )

decreased immature B cell number ( J:126113 )

increased B cell number ( J:126113 )

• 100.8+/-9.5x10⁶ compared to 46.7+/-4.8x10⁶ B cells in the spleen of wild mice

abnormal mature B cell morphology ( J:126113 )

• the ratio of B-1 to B-2 B cells is inverted compared to in wild-type mice in the peritoneal cavity

decreased B-1 B cell number ( J:126113 )

• the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)the number of B-1a and B-1b cells in the peritoneal cavity is decreased relative to in wild-type mice (0.4+/-0.1 x10⁶ compared to 1.4+/-0.3 x10⁶ in wild-type mice)

growth/size/body

enlarged spleen ( J:126113 )

Allelic Composition	Ikzf1^tm3Kge/Ikzf1^tm3Kge Tg(CD19-cre/ERT2)1Cgn/?
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ikzf1^tm3Kge mutation (0 available); any Ikzf1 mutation (30 available)
Tg(CD19-cre/ERT2)1Cgn mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell apoptosis ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cell apoptosis increases

decreased pre-B cell number ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cells rapidly decline in number

absent pre-B cells ( J:209927 )

• circulating pre-B cells are not found

cellular

abnormal B cell apoptosis ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cell apoptosis increases

immune system

abnormal B cell apoptosis ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cell apoptosis increases

decreased pre-B cell number ( J:209927 )

• after treatment with PF-562271 (inhibitor of FAK and Ptk26) large pre-B cells rapidly decline in number

absent pre-B cells ( J:209927 )

• circulating pre-B cells are not found

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