Analysis Tools
mortality/aging
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• homozygotes die immediately after birth
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limbs/digits/tail
polydactyly
(
J:90056
)
|
• at E16, homozygotes display polydactyly of the hindlimbs
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short limbs
(
J:90056
)
|
• at E16, homozygotes have short limbs relative to wild-type mice
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skeleton
|
• in mutant ribs, accelerated endochondral ossification leads to premature osteoblast differentiation
|
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• beginning at E15, homozygotes exhibit a consistently thicker bone collar relative to wild-type mice
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• histologically, E15 mutant ribs resemble E16 rather than E15 wild-type ribs; osteocalcin is detected as early as E15 in mutant ribs but remains absent in wild-type E15 and 16 ribs
• at E18, wild-type and mutant ribs are comparable in morphology, although mutant ribs have a thicker bone collar and consistently contain more trabeculae relative to wild-type
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• at E16, mutant (but not wild-type) ribs are surrounded by a thick bone collar and contain bone trabeculae in the diaphysis, indicating advanced bone formation
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• at E14 and E15, mutant ribs show a slight increase in hypertrophic chondrocytes relative to wild-type ribs
• at E16, chondrocyte hypertrophy is markedly increased in wild-type ribs but noticeably reduced in mutant ribs
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nervous system
| N |
• at E16, homozygotes do NOT display any overt neural tube defects
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cellular
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• in mutant ribs, accelerated endochondral ossification leads to premature osteoblast differentiation
|
