Phenotypes associated with this allele

• Allele Symbol: Tg(MMTVTGFA)29Rjc
• Allele Name: transgene insertion 29, Robert J Coffey
• Allele ID: MGI:3040871
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(MMTV-TGFBR2)7Hlm/? Tg(MMTVTGFA)29Rjc/?	involves: C57BL/6 * DBA	MGI:3794994
cx2	Tg(MMTVTGFA)29Rjc/0 Tg(MMTVTGFB1)46Hlm/0	involves: C57BL/6 * DBA/2	MGI:3800247
cx3	Tg(MMTVneu)202Mul/0 Tg(MMTVTGFA)29Rjc/0	involves: C57BL/6 * DBA/2 * FVB/N	MGI:3800286
tg4	Tg(MMTVTGFA)29Rjc/0	involves: C57BL/6 * DBA/2	MGI:3800199

Genotype
MGI:3794994

cx1

• Allelic Composition: Tg(MMTV-TGFBR2)7Hlm/?; Tg(MMTVTGFA)29Rjc/?
• Genetic Background: involves: C57BL/6 * DBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased mammary adenocarcinoma incidence ( J:85799 )

• 17 of 19 mice develop mammary tumors with 5 mice having tumors with invasive characteristics

• the number of invasive tumors is significantly less than mice carrying just the Tg(MMTVTGFA)29Rjc transgene

• 15 of the 17 mice with tumors had tumors in multiple mammary glands

• tumor development occurs with a median latency of 9 months

integument

increased mammary adenocarcinoma incidence ( J:85799 )

• 17 of 19 mice develop mammary tumors with 5 mice having tumors with invasive characteristics

• the number of invasive tumors is significantly less than mice carrying just the Tg(MMTVTGFA)29Rjc transgene

• 15 of the 17 mice with tumors had tumors in multiple mammary glands

• tumor development occurs with a median latency of 9 months

endocrine/exocrine glands

increased mammary adenocarcinoma incidence ( J:85799 )

• 17 of 19 mice develop mammary tumors with 5 mice having tumors with invasive characteristics

• the number of invasive tumors is significantly less than mice carrying just the Tg(MMTVTGFA)29Rjc transgene

• 15 of the 17 mice with tumors had tumors in multiple mammary glands

• tumor development occurs with a median latency of 9 months

Genotype
MGI:3800247

cx2

• Allelic Composition: Tg(MMTVTGFA)29Rjc/0; Tg(MMTVTGFB1)46Hlm/0
• Genetic Background: involves: C57BL/6 * DBA/2

endocrine/exocrine glands

mammary gland alveolar hyperplasia ( J:71696 )

♀ • widely scattered hyperplastic alveolar outgrowths are present

neoplasm

neoplasm ( J:71696 )

N • unlike mice overexpressing TGFA, mice do not exhibit mammary tumors

integument

mammary gland alveolar hyperplasia ( J:71696 )

♀ • widely scattered hyperplastic alveolar outgrowths are present

Genotype
MGI:3800286

cx3

• Allelic Composition: Tg(MMTVneu)202Mul/0; Tg(MMTVTGFA)29Rjc/0
• Genetic Background: involves: C57BL/6 * DBA/2 * FVB/N

endocrine/exocrine glands

abnormal mammary gland duct morphology ( J:35344 )

♀ • female mice exhibit abnormal ductal trees

abnormal mammary gland alveolus morphology ( J:35344 )

♀ • alveoli exhibit denser cell lining in the walls compared to Tg(MMTVTGFA)29Rjc expressing mice that exhibit cystically dilated alveoli

increased mammary gland tumor incidence ( J:35344 )

• 50% of mice exhibit mammary gland tumors at day 175 and 95% at day 250 compared to 6% of Tg(MMTVTGFA)29Rjc expressing mice and 35% of Tg(MMTVneu)202Mul expressing mice at day 250

• mice exhibit tumors that are nodular or tubular but not mixed

mammary gland hyperplasia ( J:35344 )

♀ • nulliparous female mice exhibit epithelial hyperplasia and dysplasia along with stromal inflammation

neoplasm

increased mammary gland tumor incidence ( J:35344 )

• 50% of mice exhibit mammary gland tumors at day 175 and 95% at day 250 compared to 6% of Tg(MMTVTGFA)29Rjc expressing mice and 35% of Tg(MMTVneu)202Mul expressing mice at day 250

• mice exhibit tumors that are nodular or tubular but not mixed

behavior/neurological

abnormal nursing ( J:35344 )

♀ • females cannot nurse her young

integument

abnormal mammary gland duct morphology ( J:35344 )

♀ • female mice exhibit abnormal ductal trees

abnormal mammary gland alveolus morphology ( J:35344 )

♀ • alveoli exhibit denser cell lining in the walls compared to Tg(MMTVTGFA)29Rjc expressing mice that exhibit cystically dilated alveoli

increased mammary gland tumor incidence ( J:35344 )

• 50% of mice exhibit mammary gland tumors at day 175 and 95% at day 250 compared to 6% of Tg(MMTVTGFA)29Rjc expressing mice and 35% of Tg(MMTVneu)202Mul expressing mice at day 250

• mice exhibit tumors that are nodular or tubular but not mixed

mammary gland hyperplasia ( J:35344 )

♀ • nulliparous female mice exhibit epithelial hyperplasia and dysplasia along with stromal inflammation

Genotype
MGI:3800199

tg4

• Allelic Composition: Tg(MMTVTGFA)29Rjc/0
• Genetic Background: involves: C57BL/6 * DBA/2

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:28453 )

N • male mice exhibit normal mammary and salivary gland

abnormal branching of the mammary ductal tree ( J:28453 , J:35344 , J:72085 )

♀ • after formation of tumors at 3 months, glands are small with minimal secretion in the lumen and the stroma around the glands is abundant but mitosis and dysplasia of the epithelial or stromal cells is not observed (J:28453)

♀ • stroma between the mammary glands is hyperplastic (J:28453)

♀ • at 5 months two females develop bilateral swollen, lumpy thoracic mammary glands with abundant alveolar and ductal proliferation containing abundant secretions (J:28453)

♀ • at 5 months of age, female mice exhibit epithelial and stromal proliferation (J:28453)

♀ • the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453)

♀ • female mice exhibit abnormal ductal trees (J:35344)

♀ • at 4 months of age, female mice develop numerous lumps within thoracic and inguinal areas of the mammary glands with large cystic spaces and abundant secretion within the lumen along with solid hyperplasias (J:72085)

abnormal mammary gland growth during pregnancy ( J:28453 , J:72085 )

♀ • during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild-type mice (J:28453)

♀ • after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453)

♀ • during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild-type mice (J:72085)

mammary gland duct hyperplasia ( J:28453 )

♀ • at 5 months of age

abnormal mammary gland alveolus morphology ( J:35344 )

♀ • female mice exhibit cystically dilated alveoli

mammary gland alveolar hyperplasia ( J:28453 , J:72085 )

♀ (J:28453)

♀ • at day 90 (J:72085)

increased mammary gland tumor incidence ( J:28453 , J:71696 )

• after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453)

• tumors have fibrous encapsulation with no evidence of local invasion and are composed of numerous well-formed alveoli with rare mitotic figures (J:28453)

• at 17 months, 53% of female mice exhibit solid tumors (J:71696)

increased mammary adenocarcinoma incidence ( J:28453 , J:71696 , J:72080 , J:72085 )

• the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453)

• in 40% of female mice (J:71696)

• following treatment with 0.5 mg DMBA at 21 or 56 days after birth, predominantly mammary adenocarcinomas form with occasional squamous differentiation (J:72080)

• solid hyperplasias develop into adenocarcinomas (J:72085)

mammary gland hyperplasia ( J:35344 , J:71696 )

♀ • female mice exhibit lobular development similar to a lactating wild-type mice except that the alveoli are irregular and dilated with interstitial stroma edema and a modest increase in leukocyte infiltration (J:35344)

♀ • however, nulliparous mice exhibit minimal lobular development and no stromal response (J:35344)

♀ • virgin females exhibit papillary hyperplasia (J:35344)

♀ (J:71696)

neoplasm

increased mammary gland tumor incidence ( J:28453 , J:71696 )

• after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453)

• tumors have fibrous encapsulation with no evidence of local invasion and are composed of numerous well-formed alveoli with rare mitotic figures (J:28453)

• at 17 months, 53% of female mice exhibit solid tumors (J:71696)

increased mammary adenocarcinoma incidence ( J:28453 , J:71696 , J:72080 , J:72085 )

• the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453)

• in 40% of female mice (J:71696)

• following treatment with 0.5 mg DMBA at 21 or 56 days after birth, predominantly mammary adenocarcinomas form with occasional squamous differentiation (J:72080)

• solid hyperplasias develop into adenocarcinomas (J:72085)

increased squamous cell carcinoma incidence ( J:72080 )

• following treatment with 0.5 mg DMBA at 21 or 56 days after birth, predominantly mammary adenocarcinomas form with occasional squamous differentiation

behavior/neurological

abnormal nursing ( J:28453 , J:72085 )

♀ • females cannot suckle her young (J:28453)

♀ • females cannot nurse her young (J:72085)

reproductive system

abnormal mammary gland growth during pregnancy ( J:28453 , J:72085 )

♀ • during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild-type mice (J:28453)

♀ • after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453)

♀ • during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild-type mice (J:72085)

integument

abnormal branching of the mammary ductal tree ( J:28453 , J:35344 , J:72085 )

♀ • after formation of tumors at 3 months, glands are small with minimal secretion in the lumen and the stroma around the glands is abundant but mitosis and dysplasia of the epithelial or stromal cells is not observed (J:28453)

♀ • stroma between the mammary glands is hyperplastic (J:28453)

♀ • at 5 months two females develop bilateral swollen, lumpy thoracic mammary glands with abundant alveolar and ductal proliferation containing abundant secretions (J:28453)

♀ • at 5 months of age, female mice exhibit epithelial and stromal proliferation (J:28453)

♀ • the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453)

♀ • female mice exhibit abnormal ductal trees (J:35344)

♀ • at 4 months of age, female mice develop numerous lumps within thoracic and inguinal areas of the mammary glands with large cystic spaces and abundant secretion within the lumen along with solid hyperplasias (J:72085)

abnormal mammary gland growth during pregnancy ( J:28453 , J:72085 )

♀ • during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild-type mice (J:28453)

♀ • after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453)

♀ • during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild-type mice (J:72085)

mammary gland duct hyperplasia ( J:28453 )

♀ • at 5 months of age

abnormal mammary gland alveolus morphology ( J:35344 )

♀ • female mice exhibit cystically dilated alveoli

mammary gland alveolar hyperplasia ( J:28453 , J:72085 )

♀ (J:28453)

♀ • at day 90 (J:72085)

increased mammary gland tumor incidence ( J:28453 , J:71696 )

• after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453)

• tumors have fibrous encapsulation with no evidence of local invasion and are composed of numerous well-formed alveoli with rare mitotic figures (J:28453)

• at 17 months, 53% of female mice exhibit solid tumors (J:71696)

increased mammary adenocarcinoma incidence ( J:28453 , J:71696 , J:72080 , J:72085 )

• the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453)

• in 40% of female mice (J:71696)

• following treatment with 0.5 mg DMBA at 21 or 56 days after birth, predominantly mammary adenocarcinomas form with occasional squamous differentiation (J:72080)

• solid hyperplasias develop into adenocarcinomas (J:72085)

mammary gland hyperplasia ( J:35344 , J:71696 )

♀ • female mice exhibit lobular development similar to a lactating wild-type mice except that the alveoli are irregular and dilated with interstitial stroma edema and a modest increase in leukocyte infiltration (J:35344)

♀ • however, nulliparous mice exhibit minimal lobular development and no stromal response (J:35344)

♀ • virgin females exhibit papillary hyperplasia (J:35344)

♀ (J:71696)