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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Robo1tm1Matl
targeted mutation 1, Marc Tessier-Lavigne
MGI:3043142
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Robo1tm1Matl/Robo1tm1Matl involves: 129X1/SvJ MGI:5522644
hm2
 		Robo1tm1Matl/Robo1tm1Matl involves: CD-1 MGI:3043177
cn3
 		Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Rilm/Robo2tm1Rilm
Tbx4tm1(cre)Tmj/Tbx4+ 		involves: 129 MGI:5522804
cn4
 		Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Rilm/Robo2tm1Rilm
Shhtm1(EGFP/cre)Cjt/Shh+ 		involves: 129 MGI:5522668
cn5
 		Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Rilm/Robo2tm1Rilm
Twist2tm1(cre)Dor/Twist2+ 		involves: 129 * 129X1/SvJ MGI:5522801
cx6
 		Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Mrt/Robo2tm1Mrt 		involves: 129P2/OlaHsd MGI:5470528
cx7
 		Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Mrt/Robo2tm1Mrt 		involves: 129P2/OlaHsd * C57BL/6 * CD-1 MGI:5522691
cx8
 		Robo1tm1Matl/Robo1tm1Matl
Robo3tm1Matl/Robo3tm1Matl 		involves: 129/Sv * CD-1 MGI:3043207


Genotype
MGI:5522644
hm1
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
abnormal mammary gland duct morphology ( J:106011 , J:141875 )
• end buds display enlarged subcapsular spaces, invaginated cap cell layers and disorganized luminal epithelial cells (J:106011)
• 100% penetrant phenotype of hyperplastic and disorganized ducts, including mild to severe defects in ductal lumens and disrupted growth control (J:141875)

nervous system
abnormal olfactory tract morphology ( J:119469 )
• slightly enlarged in most mice at E18

cellular

integument
abnormal mammary gland duct morphology ( J:106011 , J:141875 )
• end buds display enlarged subcapsular spaces, invaginated cap cell layers and disorganized luminal epithelial cells (J:106011)
• 100% penetrant phenotype of hyperplastic and disorganized ducts, including mild to severe defects in ductal lumens and disrupted growth control (J:141875)




Genotype
MGI:3043177
hm2
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Genetic
Background		 involves: CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:89780 )
• at E11.5 misprojecting commissural axons are seen entering the doral floor plate and growing towards the ventral ventricular zone, however by E12.5 this phenotype is no longer seen
• at E12.5 many large stalled growth cones that send out multiple filopodia are seen in homozygous mutants
abnormal spinal cord lateral column morphology ( J:89780 )
• the lateral funiculus (white matter tract) is significantly thicker in homozygous mutants compared to wild-type mice as a result of commissural axons growing further away from the floor plate

cellular
abnormal axon guidance ( J:89780 )
• at E11.5 misprojecting commissural axons are seen entering the doral floor plate and growing towards the ventral ventricular zone, however by E12.5 this phenotype is no longer seen
• at E12.5 many large stalled growth cones that send out multiple filopodia are seen in homozygous mutants




Genotype
MGI:5522804
cn3
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Rilm/Robo2tm1Rilm
Tbx4tm1(cre)Tmj/Tbx4+
Genetic
Background		 involves: 129
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo2tm1Rilm mutation (1 available); any Robo2 mutation (101 available)
Tbx4tm1(cre)Tmj mutation (0 available); any Tbx4 mutation (23 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:193400 )
• normal lung and diaphragm morphology and normal lung inflation




Genotype
MGI:5522668
cn4
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Rilm/Robo2tm1Rilm
Shhtm1(EGFP/cre)Cjt/Shh+
Genetic
Background		 involves: 129
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo2tm1Rilm mutation (1 available); any Robo2 mutation (101 available)
Shhtm1(EGFP/cre)Cjt mutation (1 available); any Shh mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:193400 )
• normal organ position, diaphragm formation and lung inflation




Genotype
MGI:5522801
cn5
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Rilm/Robo2tm1Rilm
Twist2tm1(cre)Dor/Twist2+
Genetic
Background		 involves: 129 * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo2tm1Rilm mutation (1 available); any Robo2 mutation (101 available)
Twist2tm1(cre)Dor mutation (0 available); any Twist2 mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

digestive/alimentary system
abnormal foregut morphology ( J:193400 )
• closer attachment of foregut to the body wall
small esophagus ( J:193400 )
• shorter

muscle
diaphragmatic hernia ( J:193400 )
• protrusion of the abdominal organs into the chest (stomach or liver)

respiratory system
respiratory failure ( J:193400 )
• inability to inflate lungs at birth




Genotype
MGI:5470528
cx6
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Mrt/Robo2tm1Mrt
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo2tm1Mrt mutation (2 available); any Robo2 mutation (101 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
neonatal lethality, complete penetrance ( J:193400 )
• die within minutes of birth

homeostasis/metabolism

respiratory system
short trachea ( J:193400 )
• slightly shorter
respiratory failure ( J:193400 )
• lungs fail to inflate

nervous system
abnormal axon guidance ( J:119469 )
• olfactory bulb axons from E14 explants are not repelled by SLIT proteins
abnormal olfactory tract morphology ( J:119469 )
• highly defasciculated into small axonal bundles fanned all over the ventral side of the telencephalon, at E15.5 and E18
• a subset of axons originating from the lateral bulb are still present in the normal location
abnormal spinal cord ventral commissure morphology ( J:194150 )
• at E11.5, commissural axons exhibit robust postcrossing trajectory defects with failure to project to the lateral portion of the funiculus and altered lateral and ventral funiculi ration compared with wild-type mice

cellular
abnormal axon guidance ( J:119469 )
• olfactory bulb axons from E14 explants are not repelled by SLIT proteins
decreased cell migration ( J:193400 )
• in culture, fewer E10.5 foregut cells migrate away from SLIT2 containing media

digestive/alimentary system
abnormal stomach position or orientation ( J:193400 )
• mispositioning of the stomach in the thoracic instead of the abdominal cavity
• abnormal midline position detected as early as E11.5
• more prominent at E13.5 and disrupts the diaphragm
• located at the midline and protrudes through the esophageal hiatus into the thoracic cavity in neonates
abnormal foregut morphology ( J:193400 )
• delayed separation of the foregut from the body wall
small esophagus ( J:193400 )
• short

muscle

behavior/neurological

cardiovascular system
abnormal dorsal aorta morphology ( J:193400 )
• fails to fuse by E11.5




Genotype
MGI:5522691
cx7
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Robo2tm1Mrt/Robo2tm1Mrt
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo2tm1Mrt mutation (2 available); any Robo2 mutation (101 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular

nervous system
abnormal dorsal telencephalic commissure morphology ( J:120131 )
• two large ectopic fiber bundles formed of corticocortical axons are seen on either side of the corpus callosum at E18.5
abnormal anterior commissure morphology ( J:120131 )
• displaced dorsally due to the presence of ectopic fiber bundles
abnormal innervation ( J:120131 )
• large bundles of ectopic fibers are seen crossing the ventral midline at the level of the anterior commissure and in the basal telencephalon at E18.5
• most corticofugal axons are diverted toward the midline
• only a few cortical axons reach the dorsal thalamus and those that do follow a more ventral trajectory
• virtually no corticospinal axons reach the diencephalon
• many thalamic axons abnormally invade the hypothalamus




Genotype
MGI:3043207
cx8
Allelic
Composition		 Robo1tm1Matl/Robo1tm1Matl
Robo3tm1Matl/Robo3tm1Matl
Genetic
Background		 involves: 129/Sv * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo3tm1Matl mutation (1 available); any Robo3 mutation (76 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:89438 )
• at E11.5 embryos show significant midline crossing although some still fail to cross indicating a partial suppression of the Robo3tm1Matl homozygous phenotype

cellular
abnormal axon guidance ( J:89438 )
• at E11.5 embryos show significant midline crossing although some still fail to cross indicating a partial suppression of the Robo3tm1Matl homozygous phenotype




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11/12/2024
MGI 6.24 		The Jackson Laboratory