All Phenotypes Robo3<tm1Matl> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Robo3^tm1Matl
targeted mutation 1, Marc Tessier-Lavigne
MGI:3043150

Summary

11 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Robo3^tm1Matl/Robo3^tm1Matl	either: (involves: 129/Sv * C57BL/6) or (involves: 129/Sv * CD-1)	MGI:3043193
hm2	Robo3^tm1Matl/Robo3^tm1Matl	involves: C57BL/6	MGI:4837351
hm3	Robo3^tm1Matl/Robo3^tm1Matl	Not Specified	MGI:4441337
cx4	Robo3^tm1Matl/Robo3^tm1Matl Slit1^tm1.1Matl/Slit1^tm1.1Matl Slit2^tm1Matl/Slit2^tm1Matl	involves: 129/Sv * CD-1	MGI:3043203
cx5	Robo3^tm1Matl/Robo3^tm1Matl Slit1^tm1.1Matl/Slit1^tm1.1Matl	involves: 129/Sv * CD-1	MGI:3043196
cx6	Robo3^tm1Matl/Robo3^tm1Matl Slit2^tm1Matl/Slit2^tm1Matl	involves: 129/Sv * CD-1	MGI:3043199
cx7	Robo1^tm1Matl/Robo1^tm1Matl Robo3^tm1Matl/Robo3^tm1Matl	involves: 129/Sv * CD-1	MGI:3043207
cx8	Robo2^tm1Mrt/Robo2^tm1Mrt Robo3^tm1Matl/Robo3^tm1Matl	involves: 129/Sv * CD-1	MGI:3043210
cx9	Robo3^tm1Matl/Robo3^tm1Matl Tg(Mnx1-EGFP)#Tmj/0	involves: C57BL/6 * DBA	MGI:4441377
cx10	Lhx2^tm1Dra/Lhx2⁺ Lhx9^tm1Dodd/Lhx9^tm1Dodd Robo3^tm1Matl/Robo3⁺	Not Specified	MGI:3821858
cx11	Robo3^tm1Matl/Robo3⁺ Tg(Atoh1-lacZ)1Jejo/?	Not Specified	MGI:3821857

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl
Genetic Background	either: (involves: 129/Sv * C57BL/6) or (involves: 129/Sv * CD-1)

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:89438 )

• homozygous null mice are born alive but die within a few hours

nervous system

abnormal axon guidance ( J:89438 )

• from E11.5 - E14.5 at all axial levels of the spinal cord and hindbrain commissural axons fail to cross the floor plate and instead veer away after reaching the vicinity of the floor plate

• after failing to cross the floor plate the commissural axons grow longitudinally along the ipsilateral side of the floor plate in a disorganized manner with most failing to stay close to the floor plate, some stalled close to the floor plate, and others turning rostrally, caudally, or bifurcating and growing in both directions

• in vitro assays indicate axons from homozygous mutants are prematurely Slit responsive

abnormal floor plate morphology ( J:89438 )

• the spinal cord fails to stay attached at the ventral midline during dissection indicating a thin or fragile floor plate

embryo

abnormal floor plate morphology ( J:89438 )

• the spinal cord fails to stay attached at the ventral midline during dissection indicating a thin or fragile floor plate

cellular

abnormal axon guidance ( J:89438 )

• from E11.5 - E14.5 at all axial levels of the spinal cord and hindbrain commissural axons fail to cross the floor plate and instead veer away after reaching the vicinity of the floor plate

• in vitro assays indicate axons from homozygous mutants are prematurely Slit responsive

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:165285 )

• within 8 hours of birth

nervous system

abnormal pre-Botzinger complex physiology ( J:165285 )

• at E15.5, rhythmic activity frequency in the pre-Botzinger complex is lower than in wild-type mice

• at E15.5, bilateral synchronization of rhythmic activity in the pre-Botzinger complex is absent unlike in wild-type mice

respiratory system

abnormal breathing pattern ( J:165285 )

• at P0, mice exhibit independent left and right rhythmic contractions of the diaphragm unlike wild-type mice

homeostasis/metabolism

cyanosis ( J:165285 )

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Reduced internuclear commissure in Robo3^tm1.1Ache/Robo3^tm1.1Ache Egr2^tm2(cre)Pch/Egr2⁺ and Robo3^tm1Matl/Robo3^tm1Matl mice

nervous system

abnormal brain commissure morphology ( J:158918 )

• at E14 and in newborns, virtually no axons are found to cross the midline at the level of the abducens nuclei

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl Slit1^tm1.1Matl/Slit1^tm1.1Matl Slit2^tm1Matl/Slit2^tm1Matl
Genetic Background	involves: 129/Sv * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)
Slit1^tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2^tm1Matl mutation (2 available); any Slit2 mutation (88 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal axon guidance ( J:89438 )

• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3^tm1Matl homozygous phenotype

cellular

abnormal axon guidance ( J:89438 )

• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3^tm1Matl homozygous phenotype

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl Slit1^tm1.1Matl/Slit1^tm1.1Matl
Genetic Background	involves: 129/Sv * CD-1

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal axon guidance ( J:89438 )

• embryos show no midline crossing and look identical to Robo3^tm1Matl homozygous littermates

cellular

abnormal axon guidance ( J:89438 )

• embryos show no midline crossing and look identical to Robo3^tm1Matl homozygous littermates

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl Slit2^tm1Matl/Slit2^tm1Matl
Genetic Background	involves: 129/Sv * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)
Slit2^tm1Matl mutation (2 available); any Slit2 mutation (88 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal axon guidance ( J:89438 )

• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3^tm1Matl homozygous phenotype

cellular

abnormal axon guidance ( J:89438 )

• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3^tm1Matl homozygous phenotype

Allelic Composition	Robo1^tm1Matl/Robo1^tm1Matl Robo3^tm1Matl/Robo3^tm1Matl
Genetic Background	involves: 129/Sv * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo1^tm1Matl mutation (2 available); any Robo1 mutation (87 available)
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal axon guidance ( J:89438 )

• at E11.5 embryos show significant midline crossing although some still fail to cross indicating a partial suppression of the Robo3^tm1Matl homozygous phenotype

cellular

abnormal axon guidance ( J:89438 )

• at E11.5 embryos show significant midline crossing although some still fail to cross indicating a partial suppression of the Robo3^tm1Matl homozygous phenotype

Allelic Composition	Robo2^tm1Mrt/Robo2^tm1Mrt Robo3^tm1Matl/Robo3^tm1Matl
Genetic Background	involves: 129/Sv * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo2^tm1Mrt mutation (2 available); any Robo2 mutation (101 available)
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal axon guidance ( J:89438 )

• at E11.5 embryos show no midline crossing and look identical to Robo3^tm1Matl homozygous littermates

cellular

abnormal axon guidance ( J:89438 )

• at E11.5 embryos show no midline crossing and look identical to Robo3^tm1Matl homozygous littermates

Allelic Composition	Robo3^tm1Matl/Robo3^tm1Matl Tg(Mnx1-EGFP)#Tmj/0
Genetic Background	involves: C57BL/6 * DBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)
Tg(Mnx1-EGFP)#Tmj mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal pons morphology ( J:158918 )

• at E14, the abducens motor nucleus is closer to the floor plate and abnormally shaped

Allelic Composition	Lhx2^tm1Dra/Lhx2⁺ Lhx9^tm1Dodd/Lhx9^tm1Dodd Robo3^tm1Matl/Robo3⁺
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lhx2^tm1Dra mutation (0 available); any Lhx2 mutation (12 available)
Lhx9^tm1Dodd mutation (0 available); any Lhx9 mutation (31 available)
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal dorsal interneuron 1 morphology ( J:142117 )

• a 61% reduction in midline cross over of axons from dl1c neurons

Allelic Composition	Robo3^tm1Matl/Robo3⁺ Tg(Atoh1-lacZ)1Jejo/?
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3^tm1Matl mutation (1 available); any Robo3 mutation (76 available)
Tg(Atoh1-lacZ)1Jejo mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal dorsal interneuron 1 morphology ( J:142117 )

• some dl1 neurons migrate ectopically to a more ventral position

• a modest 11% reduction in midline cross over of axons from dl1c neurons

• project instead along the ipsilateral ventral funiculus

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