Phenotypes associated with this allele

• Allele Symbol: Trp53^tm1Elee
• Allele Name: targeted mutation 1, Eva YHP Lee
• Allele ID: MGI:3043426
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nf1^tm1Par/Nf1^+ Pten^tm1Hwu/Pten^+ Trp53^tm1Elee/Trp53^+ Tg(GFAP-cre)25Mes/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * FVB/N	MGI:4840096
cn2	Nf1^tm1Par/Nf1^+ Trp53^tm1Elee/Trp53^+ Tg(GFAP-cre)25Mes/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * FVB/N	MGI:4840095
cn3	Trp53^tm1Elee/Trp53^tm1Tyj Tg(GFAP-cre)25Mes/0	involves: 129S2/SvPas * 129S4/SvJae * FVB/N	MGI:3849178
cn4	Trp53^tm1Elee/Trp53^tm1Elee Tg(Wap-rtTA-cre)10Whl/0	involves: 129S4/SvJae * C57BL/6	MGI:3044060
cn5	Trp53^tm1Elee/Trp53^tm1Elee Tg(MMTV-cre)AElee/0	involves: 129S4/SvJae * C57BL/6	MGI:3044051
cn6	Trp53^tm1Elee/Trp53^+ Tg(MMTV-cre)AElee/0	involves: 129S4/SvJae * C57BL/6	MGI:3044053
cn7	Trp53^tm1Elee/Trp53^tm1Elee Tg(MMTV-cre)BElee/0	involves: 129S4/SvJae * C57BL/6	MGI:3044055
cn8	Trp53^tm1Elee/Trp53^tm1Elee Tg(GFAP-cre)25Mes/0	involves: 129S4/SvJae * FVB/N	MGI:3849177
cn9	Nf1^tm1Fcr/Nf1^tm1Fcr Trp53^tm1Elee/Trp53^tm1Elee Tg(GFAP-cre)25Mes/0	involves: 129S/SvEv * 129S4/SvJae * FVB/N	MGI:3849179

Genotype
MGI:4840096

cn1

• Allelic Composition: Nf1^tm1Par/Nf1⁺; Pten^tm1Hwu/Pten⁺; Trp53^tm1Elee/Trp53⁺; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:134611 )

• most die as early as 10 weeks of age, and usually within a week of showing signs of morbidity

behavior/neurological

increased startle reflex ( J:134611 )

• exaggerated startle response

ataxia ( J:134611 )

• ataxic gait

unidirectional circling ( J:134611 )

seizures ( J:134611 )

• generalized motor seizures

nervous system

seizures ( J:134611 )

• generalized motor seizures

increased brain tumor incidence ( J:134611 )

increased astrocytoma incidence ( J:134611 )

• mutants develop astrocytomas

• neurospheres from mutant astrocytomas exhibit loss of heterozygosity at both Nf1 and Trp53 loci

neoplasm

increased brain tumor incidence ( J:134611 )

increased astrocytoma incidence ( J:134611 )

• mutants develop astrocytomas

• neurospheres from mutant astrocytomas exhibit loss of heterozygosity at both Nf1 and Trp53 loci

Genotype
MGI:4840095

cn2

• Allelic Composition: Nf1^tm1Par/Nf1⁺; Trp53^tm1Elee/Trp53⁺; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * FVB/N

mortality/aging

premature death ( J:134611 )

• mutants survive up to 8 weeks beyond initial appearance of symptoms

behavior/neurological

increased startle reflex ( J:134611 )

• exaggerated startle response

ataxia ( J:134611 )

• ataxic gait

unidirectional circling ( J:134611 )

seizures ( J:134611 )

• generalized motor seizures

nervous system

seizures ( J:134611 )

• generalized motor seizures

increased brain tumor incidence ( J:134611 )

increased astrocytoma incidence ( J:134611 )

• mutants develop astrocytomas

• neurospheres from mutant astrocytomas exhibit loss of heterozygosity at both Nf1 and Trp53 loci

neoplasm

increased brain tumor incidence ( J:134611 )

increased astrocytoma incidence ( J:134611 )

• mutants develop astrocytomas

• neurospheres from mutant astrocytomas exhibit loss of heterozygosity at both Nf1 and Trp53 loci

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
malignant astrocytoma		DOID:3069		J:134611

Genotype
MGI:3849178

cn3

• Allelic Composition: Trp53^tm1Elee/Trp53^tm1Tyj; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * FVB/N

mortality/aging

premature death ( J:149662 )

• all mice die prior to 500 days

behavior/neurological

tremors ( J:149662 )

• 85% of mice

ataxia ( J:149662 )

• 85% of mice

impaired balance ( J:149662 )

• 85% of mice

seizures ( J:149662 )

• 85% of mice

neoplasm

increased medulloblastoma incidence ( J:149662 )

• 90% of mice develop medulloblastomas

increased sarcoma incidence ( J:149662 )

• mice develop tumors outside of the central nervous system consisting of soft-tissue sarcomas

increased glioma incidence ( J:149662 )

• 90% of mice develop malignant gliomas with astrocytic characteristics

• 40% of high-grade astrocytic gliomas exhibit necrosis, pseudopalisading tumors cells, high degree of nuclear atypia, and microvascular proliferation similar to human glioblastoma multiforme

• tumors are relatively heterogeneous histological and in lineage marker expression

nervous system

nervous system phenotype ( J:149662 )

N • at 2 months of age, brain cells in the subventricular zone and progenitor cells exhibit normal growth rates

seizures ( J:149662 )

• 85% of mice

increased medulloblastoma incidence ( J:149662 )

• 90% of mice develop medulloblastomas

increased glioma incidence ( J:149662 )

• 90% of mice develop malignant gliomas with astrocytic characteristics

• 40% of high-grade astrocytic gliomas exhibit necrosis, pseudopalisading tumors cells, high degree of nuclear atypia, and microvascular proliferation similar to human glioblastoma multiforme

• tumors are relatively heterogeneous histological and in lineage marker expression

increased brain size ( J:149662 )

• in mice with neurological defects

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
glioblastoma		DOID:3068		J:149662

Genotype
MGI:3044060

cn4

• Allelic Composition: Trp53^tm1Elee/Trp53^tm1Elee; Tg(Wap-rtTA-cre)10Whl/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6

neoplasm

increased tumor incidence ( J:90247 )

• mean tumor latency about 9.5 months

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammalian tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

endocrine/exocrine glands

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammalian tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

integument

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammalian tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

Genotype
MGI:3044051

cn5

• Allelic Composition: Trp53^tm1Elee/Trp53^tm1Elee; Tg(MMTV-cre)AElee/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6

neoplasm

increased mammary gland tumor incidence ( J:90247 )

• most common tumors were mammary tumors

• 60-80% of those with mammary tumors had multiple primary tumors

• nulliparous females developed tumors between 14 and 24 months of age, averaging 17.5 months

• multiparous females with shortened latency, develop between 11.5 and 24 months with an average of 15.5 months

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammary tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

• about 50% with metastasis to lung or liver

increased lymphoma incidence ( J:90247 )

• some lymphomas occur

integument

increased mammary gland tumor incidence ( J:90247 )

• most common tumors were mammary tumors

• 60-80% of those with mammary tumors had multiple primary tumors

• nulliparous females developed tumors between 14 and 24 months of age, averaging 17.5 months

• multiparous females with shortened latency, develop between 11.5 and 24 months with an average of 15.5 months

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammary tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

• about 50% with metastasis to lung or liver

endocrine/exocrine glands

increased mammary gland tumor incidence ( J:90247 )

• most common tumors were mammary tumors

• 60-80% of those with mammary tumors had multiple primary tumors

• nulliparous females developed tumors between 14 and 24 months of age, averaging 17.5 months

• multiparous females with shortened latency, develop between 11.5 and 24 months with an average of 15.5 months

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammary tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

• about 50% with metastasis to lung or liver

Genotype
MGI:3044053

cn6

• Allelic Composition: Trp53^tm1Elee/Trp53⁺; Tg(MMTV-cre)AElee/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6

neoplasm

increased tumor latency ( J:90247 )

• latency was very long with very low penetrance

Genotype
MGI:3044055

cn7

• Allelic Composition: Trp53^tm1Elee/Trp53^tm1Elee; Tg(MMTV-cre)BElee/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6

neoplasm

increased tumor incidence ( J:90247 )

• mean tumor latency about 11 months but with lower penetrance due to other tumor types

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammalian tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

integument

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammalian tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

endocrine/exocrine glands

increased mammary adenocarcinoma incidence ( J:90247 )

• all mammalian tumors were adenocarcinomas, myoepithelial adenocarcinomas, adenosquamous carcinomas, or spindle cell tumors

• mostly poorly differentiated and invasive

Genotype
MGI:3849177

cn8

• Allelic Composition: Trp53^tm1Elee/Trp53^tm1Elee; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S4/SvJae * FVB/N

mortality/aging

premature death ( J:149662 )

• all mice die prior to 400 days

behavior/neurological

tremors ( J:149662 )

• 84% of mice

ataxia ( J:149662 )

• 84% of mice

impaired balance ( J:149662 )

• 84% of mice

seizures ( J:149662 )

• 84% of mice

neoplasm

increased medulloblastoma incidence ( J:149662 )

• 90% of mice develop medulloblastomas

increased sarcoma incidence ( J:149662 )

• mice develop tumors outside of the central nervous system consisting of soft-tissue sarcomas

increased glioma incidence ( J:149662 )

• 90% of mice develop malignant gliomas with astrocytic characteristics

• 40% of high-grade astrocytic gliomas exhibit necrosis, pseudopalisading tumors cells, high degree of nuclear atypia, and microvascular proliferation similar to human glioblastoma multiforme

• tumors are relatively heterogeneous histological and in lineage marker expression

nervous system

nervous system phenotype ( J:149662 )

N • at 2 months of age, brain cells in the subventricular zone and progenitor cells exhibit normal growth rates

seizures ( J:149662 )

• 84% of mice

increased medulloblastoma incidence ( J:149662 )

• 90% of mice develop medulloblastomas

increased glioma incidence ( J:149662 )

• 90% of mice develop malignant gliomas with astrocytic characteristics

• 40% of high-grade astrocytic gliomas exhibit necrosis, pseudopalisading tumors cells, high degree of nuclear atypia, and microvascular proliferation similar to human glioblastoma multiforme

• tumors are relatively heterogeneous histological and in lineage marker expression

increased brain size ( J:149662 )

• in mice with neurological defects

Genotype
MGI:3849179

cn9

• Allelic Composition: Nf1^tm1Fcr/Nf1^tm1Fcr; Trp53^tm1Elee/Trp53^tm1Elee; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S/SvEv * 129S4/SvJae * FVB/N

mortality/aging

premature death ( J:149662 )

• all mice die prior to 200 days

neoplasm

increased glioma incidence ( J:149662 )

• 70% of gliomas exhibit necrosis, a feature of human glioblastoma multiforme

• tumors are relatively homogeneous histological and in lineage marker expression

decreased tumor latency ( J:149662 )

• all mice develop astrocytic gliomas with shorter latency than observed in Trp53^tm1Elee/Trp53^tm1Tyj Tg(GFAP-cre)25Mes or Trp53^tm1Elee/ Trp53^tm1Elee Tg(GFAP-cre)25Mes mice

nervous system

increased glioma incidence ( J:149662 )

• 70% of gliomas exhibit necrosis, a feature of human glioblastoma multiforme

• tumors are relatively homogeneous histological and in lineage marker expression

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
glioblastoma		DOID:3068		J:149662