Phenotypes associated with this allele

• Allele Symbol: Tg(Ins1-Cat,Tyr)25Pne
• Allele Name: transgene insertion 25, Paul N Epstein
• Allele ID: MGI:3043843
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Ins1-Cat,Tyr)25Pne/0	involves: FVB	MGI:3623483
tg2	Tg(Ins1-Cat,Tyr)25Pne/0	NOD.FVB-Tg(Ins1-Cat,Tyr)25Pne	MGI:3624036

Genotype
MGI:3623483

tg1

• Allelic Composition: Tg(Ins1-Cat,Tyr)25Pne/0
• Genetic Background: involves: FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased insulin secretion ( J:59275 )

• interleukin-1-beta treatment of islets from transgenic mice reduces the insulin content or stimulate insulin secretion similarly to control islet cells

decreased circulating glucose level ( J:59275 )

• for the first 3 days after streptozocin injection, transgenic mice have decreased blood glucose levels compared to injected control mice; by day 4 transgenic and control mice are diabetic

increased catalase activity ( J:59275 )

• catalase activity in pancreatic islet cells transgenic mice is 50-fold increased over levels in control islet cells

abnormal physiological response to xenobiotic ( J:59275 )

• in response to streptozocin, a beta-cell toxin, transgenic mice display a significantly delayed diabetogenic to a single injection, compared to control mice

endocrine/exocrine glands

increased insulin secretion ( J:59275 )

• interleukin-1-beta treatment of islets from transgenic mice reduces the insulin content or stimulate insulin secretion similarly to control islet cells

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:59725

Genotype
MGI:3624036

tg2

• Allelic Composition: Tg(Ins1-Cat,Tyr)25Pne/0
• Genetic Background: NOD.FVB-Tg(Ins1-Cat,Tyr)25Pne

homeostasis/metabolism

increased physiological sensitivity to xenobiotic ( J:108415 )

• treatment of transgenic NOD mice with cyclophosphamide (CYP) accelerated NOD diabetes; 20 days after injection of CYP 63% of transgenic mice are diabetic compared with 7% of treated controls

immune system

increased susceptibility to autoimmune diabetes ( J:108415 )

• transgenic NOD male mice display a significant acceleration of the onset of spontaneous diabetes (over 85% at 200 days of age) compared to NOD controls (30-40% of male NOD mice)

endocrine/exocrine glands

abnormal pancreatic islet morphology ( J:108415 )

• cultured islets of transgenic NOD mice are more sensitive to a mix of cytokines (Il-beta, Tnfa and Ifng) than NOD control islets as revealed by Caspase-3 activity

decreased pancreatic beta cell number ( J:108415 )

• beta cell damage is significantly increased in transgenic mice after CYP injection compared to controls; pancreatic insulin content is only 22% of original level after 8 days compared to 76% in control NOD mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:108415