Phenotypes associated with this allele

• Allele Symbol: Mlxipl^tm1Kuy
• Allele Name: targeted mutation 1, Kosaku Uyeda
• Allele ID: MGI:3043871
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mlxipl^tm1Kuy/Mlxipl^tm1Kuy	B6.129S6-Mlxipl^tm1Kuy/J	MGI:6297092
hm2	Mlxipl^tm1Kuy/Mlxipl^tm1Kuy	involves: 129S6/SvEvTac * C57BL/6J	MGI:3043880

Genotype
MGI:6297092

hm1

• Allelic Composition: Mlxipl^tm1Kuy/Mlxipl^tm1Kuy
• Genetic Background: B6.129S6-Mlxipl^tm1Kuy/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

weight loss ( J:273754 )

• mice exhibit a significant weight loss when fed a high fructose diet (60% fructose, 10% starch), showing a 20% reduction in body weight after 5 days on the diet

• body weight recovers within 6 days after switching back to the control diet

digestive/alimentary system

diarrhea ( J:273754 )

• mice fed a high fructose diet develop severe diarrhea

increased cecum weight ( J:273754 )

• cecum weight is increased in mice fed a high fructose diet

distended cecum ( J:273754 )

• on day 3 of a high fructose diet, mice exhibit enlarged ceca that contains fluid

• by day 5 and through day 7 of the high fructose diet, mice exhibit enlarged, red intestines and extremely distended ceca that contain both fluid and gas

increased colon length ( J:273754 )

• colon length is increased in mice fed a high fructose diet

decreased defecation amount ( J:273754 )

• fecal pellets are decreased in high fructose fed mice

abnormal intestinal fructose absorption ( J:273754 )

• fructose levels in feces are 2-fold higher in mice fed a high fructose diet, indicating development of fructose intolerance

• plasma alanine transaminase, aspartate transaminase, and lactate dehydrogenase, indicators of liver injury, are not increased in mutant mice fed a high fructose diet unlike in wild-type mice that show an increase in their levels, indicating resistance to liver damage by high fructose diet due to impaired fructose absorption

• on the high fructose diet, lactate levels in the intestine are not increased as in wild-type mice and lactate levels in the liver are decreased in mutants indicating impaired fructose conversion into lactate in the intestine but not the liver

behavior/neurological

decreased food intake ( J:273754 )

• average daily food intake is lower in mice fed a high fructose diet

• food intake is decreased by more than 50% within 1-2 days after starting the high fructose diet and increases right after switching back to the control diet

homeostasis/metabolism

decreased circulating glucose level ( J:273754 )

♂ • males exhibit a lower blood glucose levels when fed a high fructose diet

increased circulating glucose level ( J:273754 )

♀ • females exhibit a slight increase in blood glucose levels when fed a high fructose diet

decreased circulating cholesterol level ( J:273754 )

• plasma cholesterol levels are decreased in mutant mice on both a control diet and the high fructose diet

decreased circulating triglyceride level ( J:273754 )

• plasma triglyceride levels are decreased in mutant mice on both a control diet and the high fructose diet

decreased body temperature ( J:273754 )

• mice exhibit lower body temperature when fed a high fructose diet

decreased liver triglyceride level ( J:273754 )

• liver triglyceride levels are decreased in mutant mice on both a control diet and the high fructose diet

liver/biliary system

decreased liver triglyceride level ( J:273754 )

• liver triglyceride levels are decreased in mutant mice on both a control diet and the high fructose diet

mortality/aging

premature death ( J:273754 )

• mice fed a high fructose diet become moribund in 2 weeks

• mice younger than 5 weeks that are fed the high fructose diet become moribund within a few days

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
irritable bowel syndrome		DOID:9778		J:273754

Genotype
MGI:3043880

hm2

• Allelic Composition: Mlxipl^tm1Kuy/Mlxipl^tm1Kuy
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:89865 )

• on a 70% sucrose diet homozygous mice die in less than 1 week

• on a high-fructose diet homozygous mice die within a few days

• on a standard chow diet homozygous mice have a normal life span

cellular

asthenozoospermia ( J:324060 )

♂

immotile sperm ( J:324060 )

♂

growth/size/body

increased liver weight ( J:89865 )

• on a high starch diet liver weight are about 40% greater in homozygous mice compared to wild-type mice probably as a result of increased glycogen stores in homozygous mice

adipose tissue

decreased brown adipose tissue amount ( J:89865 )

• on a standard diet homozygous mice have lower brown fat weights

decreased white adipose tissue amount ( J:89865 )

decreased epididymal fat pad weight ( J:89865 )

• on a standard diet homozygous mice have lower epididymal fat weights

homeostasis/metabolism

decreased body temperature ( J:89865 )

• on a 70% sucrose diet homozygous mice develop hypothermia

abnormal glucose homeostasis ( J:89865 )

increased circulating glucose level ( J:89865 )

• plasma glucose levels are somewhat elevated

increased circulating insulin level ( J:89865 )

• plasma insulin levels are somewhat elevated

• on a high-starch diet plasma insulin levels increase significantly compared to homozygous mice on a standard diet or wild-type mice on either diet

increased liver glycogen level ( J:89865 )

• liver glycogen levels are elevated but muscle glycogen levels are not elevated

• liver glycogen levels are elevated further on a high-starch diet

insulin resistance ( J:89865 )

• on a high-starch diet homozygous mice are moderately insulin resistant

abnormal lipid homeostasis ( J:89865 )

decreased circulating cholesterol level ( J:89865 )

• on a high starch diet plasma cholesterol is significantly lower compared to wild-type mice on the same diet

decreased fatty acids level ( J:89865 )

• hepatic fatty acid synthesis rates are 65% lower in homozygous mice

decreased circulating free fatty acids level ( J:89865 )

• on either a standard or high-starch diet plasma free fatty acid levels are about 50% that in wild-type mice on the same diet

• on a 70% sucrose diet free fatty acid levels decline further in homozygous mice

reproductive system

asthenozoospermia ( J:324060 )

♂

immotile sperm ( J:324060 )

♂

male infertility ( J:324060 )

♂

liver/biliary system

increased liver weight ( J:89865 )

• on a high starch diet liver weight are about 40% greater in homozygous mice compared to wild-type mice probably as a result of increased glycogen stores in homozygous mice

increased liver glycogen level ( J:89865 )

• liver glycogen levels are elevated but muscle glycogen levels are not elevated

• liver glycogen levels are elevated further on a high-starch diet

abnormal liver physiology ( J:89865 )

• hepatic glucose levels are increased

• hepatic pyruvate/phosphoenolpyruvate ratio is decreased indicating inhibition of hepatic glycolysis