All Phenotypes Tg(ACTB-NOTCH1)1Shn MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(ACTB-NOTCH1)1Shn
transgene insertion 1, Jie Shen
MGI:3044583

Summary

5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tg(Nes-cre)1Kln/0 Tg(ACTB-NOTCH1)1Shn/0	B6.Cg-Tg(Nes-cre)1Kln Tg(ACTB-NOTCH1)1Shn	MGI:3044595
cn2	Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * C57BL/6J	MGI:3044601
cn3	Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53^tm1Tyj/Trp53⁺	involves: 129S2/SvPas * C57BL/6J	MGI:3044602
cn4	Tg(ACTB-NOTCH1)1Shn/? Tg(Nes-cre/Esr1*)4Ynj/?	involves: C57BL/6 * C57BL/6J	MGI:3765969
cn5	Tg(ACTB-NOTCH1)1Shn/0 Tg(Syn1-cre)671Jxm/0	involves: C57BL/6J	MGI:3044599

Allelic Composition	Tg(Nes-cre)1Kln/0 Tg(ACTB-NOTCH1)1Shn/0
Genetic Background	B6.Cg-Tg(Nes-cre)1Kln Tg(ACTB-NOTCH1)1Shn

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ACTB-NOTCH1)1Shn mutation (1 available)
Tg(Nes-cre)1Kln mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:90392 )

prenatal lethality, incomplete penetrance ( J:90392 )

• some of the mutants die before birth; however, most survive to the perinatal stage

nervous system

abnormal neuron apoptosis ( J:90392 )

• at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased

• by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive

decreased cortical ventricular zone thickness ( J:90392 )

• at E12 - 13.5 the ventricular zone harboring neural progenitors is thinner

small embryonic telencephalon ( J:90392 )

• at E12 - 13.5 the telencephalon is smaller compared to wild-type or single transgenic littermates

decreased brain size ( J:90392 )

• at E12 - 13.5 all brain subregions are smaller compared to wild-type or single transgenic littermates

• at P0 the brain especially the cerebral cortex is much smaller in double transgenic mice compared to wild-type or single transgenic littermates

decreased neuron number ( J:90392 )

• the number of postmitotic neurons is decreased at E12 - 13.5 in the telencephalon and diencephalon

cellular

abnormal neuron apoptosis ( J:90392 )

• at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased

• by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive

Allelic Composition	Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53^tm1Tyj/Trp53^tm1Tyj
Genetic Background	involves: 129S2/SvPas * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ACTB-NOTCH1)1Shn mutation (1 available)
Tg(Nes-cre)1Kln mutation (4 available)
Trp53^tm1Tyj mutation (12 available); any Trp53 mutation (240 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

prenatal lethality, incomplete penetrance ( J:90392 )

• fewer than expected double transgenic homozygous mutants are found due to incomplete penetrance of Trp53^tm1Tyj embryonic lethality

cellular

cellular phenotype ( J:90392 )

N	• at E10 - 11.5 no increase in the number of apoptotic cells in the brain is found unlike in double transgenics with wild-type Trp53

Allelic Composition	Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53^tm1Tyj/Trp53⁺
Genetic Background	involves: 129S2/SvPas * C57BL/6J

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal apoptosis ( J:90392 )

• at E10 - 11.5 the number of apoptotic cells in the brain is increased

Allelic Composition	Tg(ACTB-NOTCH1)1Shn/? Tg(Nes-cre/Esr1*)4Ynj/?
Genetic Background	involves: C57BL/6 * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ACTB-NOTCH1)1Shn mutation (1 available)
Tg(Nes-cre/Esr1*)4Ynj mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:127227 )

N	• no enlargement of lateral ventricles or defects in ependymal adhesion of ventricular walls are observed

increased neuron apoptosis ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast apoptosis at the striato-cortical junction

abnormal postnatal subventricular zone morphology ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast cellularity at the striato-cortical junction

cellular

increased neuron apoptosis ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast apoptosis at the striato-cortical junction

Allelic Composition	Tg(ACTB-NOTCH1)1Shn/0 Tg(Syn1-cre)671Jxm/0
Genetic Background	involves: C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ACTB-NOTCH1)1Shn mutation (1 available)
Tg(Syn1-cre)671Jxm mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

cellular phenotype ( J:90392 )

N	• no increase in apoptosis is seen at E13.5 in the telencephalon of double transgenic mice

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