Phenotypes associated with this allele

• Allele Symbol: Spi1^tm1Kast
• Allele Name: targeted mutation 1, Philippe Kastner
• Allele ID: MGI:3044765
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Spi1^tm1Kast/Spi1^tm1Kast	B6.129S2-Spi1^tm1Kast	MGI:3044767
ht2	Spi1^tm1Kast/Spi1^+	B6.129S2-Spi1^tm1Kast	MGI:3044769

Genotype
MGI:3044767

hm1

• Allelic Composition: Spi1^tm1Kast/Spi1^tm1Kast
• Genetic Background: B6.129S2-Spi1^tm1Kast

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:90543 )

• homozygous mutants die within 1 day of birth

hematopoietic system

abnormal erythropoiesis ( J:90543 )

• from E11.5 - E12.5 the cellularity of homozygous mutant fetal livers is about 50% that of wild-type this difference is gone by E13.5

• in culture erythroid progenitors from homozygous mutants do not increase in number and produce disproportionately more mature erythroid cells compared to cells from wild-type mice

failure of myelopoiesis ( J:90543 )

• myeloid lineage cells are absent from the fetal liver of homozygous mutants

abnormal fetal derived definitive erythrocyte cell number ( J:90543 )

• at E12.5 fetal livers from homozygous mutants contain fewer mature erythroid cells

• by E15.5 however the number of mature erythroid cells is significantly higher in homozygous mutant fetal livers

absent B cells ( J:90543 )

• B-lineage cells are absent from the fetal liver of homozygous mutants

immune system

failure of myelopoiesis ( J:90543 )

• myeloid lineage cells are absent from the fetal liver of homozygous mutants

absent B cells ( J:90543 )

• B-lineage cells are absent from the fetal liver of homozygous mutants

Genotype
MGI:3044769

ht2

• Allelic Composition: Spi1^tm1Kast/Spi1⁺
• Genetic Background: B6.129S2-Spi1^tm1Kast

hematopoietic system

abnormal erythropoiesis ( J:90543 )

• 4 and 5 days after induced erythrocyte lysis spleens from heterozygous mice contain more mature precursors (CFU-Es) and mature erythroid (TER119⁺) cells

• prior to lysis no difference in cell numbers is detected

increased bone marrow cell number ( J:196609 )

increased hematopoietic stem cell number ( J:196609 )