All Phenotypes Cnot7<tm1Tno> MGI Mouse

oligozoospermia ( J:90439 )

• homozygous males produce only 7% as much sperm as wild-type mice

teratozoospermia ( J:90439 )

• spermatozoa from homozygous males have irregularly shaped heads, abnormally arranged mitochondria, and improperly attached flagella

• transfer of wild-type spermatogonial stem cells into homozygous mutants results in the production of abnormal spermatozoa

abnormal sperm flagellum morphology ( J:90439 )

• improperly attached flagella

abnormal sperm mitochondrial sheath morphology ( J:90439 )

• abnormally arranged mitochondria

abnormal sperm head morphology ( J:90439 )

• abnormal head shapes include round (73%), tapered (20%), and symplast (5%)

• 2% of sperm heads are nearly normal

abnormal spermatid morphology ( J:90439 )

• malformed spermatids, unsynchronized maturation of spermatids, and a reduction in the number of late spermatids are seen in homozygous males

multinucleated giant male germ cells ( J:90439 )

• multinucleated germ cells are observed

increased male germ cell apoptosis ( J:90439 )

• an increase in apoptotic male germ cells is observed

asthenozoospermia ( J:90439 )

• spermatozoa beat less vigerously, generate less forward momentum and are unable to penetrate eggs with intact cumulus cell layers and zona pellucida in vitro

abnormal Sertoli cell morphology ( J:90439 )

• lipids accumulate in the cytoplasm of Sertoli cells

seminiferous tubule degeneration ( J:90439 )

• in the seminiferous tubules from homozygous males large, round, clear vacuoles and in severe cases loss of most germ cells are seen

small testis ( J:90439 )

• testes from homozygous males are smaller than those from wild-type or heterozygous males

male infertility ( J:90439 )

• males are sterile however spermatids from homozygous males can successfully fertilize eggs using intracytoplasmic sperm injection

• females produce normal size litters