Phenotypes associated with this allele

• Allele Symbol: Jak2^tm1Kuw
• Allele Name: targeted mutation 1, Kay-Uwe Wagner
• Allele ID: MGI:3045140
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Jak2^tm1Kuw/Jak2^tm1Kuw	involves: 129X1/SvJ	MGI:3045184
cn2	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(Wap-cre)11738Mam/0	involves: 129X1/SvJ * C57BL/6 * FVB	MGI:3045814
cn3	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(MMTV-cre)1Mam/0	involves: 129X1/SvJ * C57BL/6 * FVB	MGI:3045399
cn4	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(Gnrh1-cre)35Awo/0	involves: 129X1/SvJ * CD-1	MGI:6856578

Genotype
MGI:3045184

hm1

• Allelic Composition: Jak2^tm1Kuw/Jak2^tm1Kuw
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:90883 )

• homozygous mice developed normally and were fertile

Genotype
MGI:3045814

cn2

• Allelic Composition: Jak2^tm1Kuw/Jak2^tm1Kuw; Tg(Wap-cre)11738Mam/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * FVB

endocrine/exocrine glands

abnormal mammary gland growth during lactation ( J:90883 )

♀ • differentiated mammary epithelia that expressed the Tg(Wap-cre)11738Mam transgene were not maintained during lactation; these Jak2 deficient cells appeared during the first few days of lactation, then were absent for the remainder of the lactation period

integument

abnormal mammary gland growth during lactation ( J:90883 )

♀ • differentiated mammary epithelia that expressed the Tg(Wap-cre)11738Mam transgene were not maintained during lactation; these Jak2 deficient cells appeared during the first few days of lactation, then were absent for the remainder of the lactation period

Genotype
MGI:3045399

cn3

• Allelic Composition: Jak2^tm1Kuw/Jak2^tm1Kuw; Tg(MMTV-cre)1Mam/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * FVB

endocrine/exocrine glands

abnormal mammary gland development ( J:90883 )

♀ • absence of the lobular units that normally reside on the terminal ducts of the tertiary side branches

♀ • lack of alveolar precursors results in impairment development of secretory lobules

abnormal mammary gland growth during pregnancy ( J:90883 )

♀ • mammary epithelia did not undergo alveolar specification during pregnancy

lactation failure ( J:90883 )

♀ • failure of dams to lactate; multiple pregnancies did not restore milk production

reproductive system

abnormal mammary gland growth during pregnancy ( J:90883 )

♀ • mammary epithelia did not undergo alveolar specification during pregnancy

integument

abnormal mammary gland development ( J:90883 )

♀ • absence of the lobular units that normally reside on the terminal ducts of the tertiary side branches

♀ • lack of alveolar precursors results in impairment development of secretory lobules

abnormal mammary gland growth during pregnancy ( J:90883 )

♀ • mammary epithelia did not undergo alveolar specification during pregnancy

lactation failure ( J:90883 )

♀ • failure of dams to lactate; multiple pregnancies did not restore milk production

Genotype
MGI:6856578

cn4

• Allelic Composition: Jak2^tm1Kuw/Jak2^tm1Kuw; Tg(Gnrh1-cre)35Awo/0
• Genetic Background: involves: 129X1/SvJ * CD-1

reproductive system

decreased corpora lutea number ( J:167849 )

♀ • number of corpora lutea is severely decreased at 4 months of age

decreased ovary weight ( J:167849 )

♀ • ovary weight is significantly decreased at 4 months of age

delayed female fertility ( J:167849 )

♀ • when mated with wild-type male mice for 90 days, females bear their first litter significantly later than control females

delayed sexual maturation ( J:167849 )

♀ • females display delayed puberty onset: both the time of vaginal opening and the time of first estrus (defined as 100% cornified cells) are significantly delayed

delayed vaginal opening ( J:167849 )

♀ • time of vaginal opening is significantly delayed

decreased ovulation rate ( J:167849 )

♀

abnormal estrous cycle ( J:167849 )

♀ • during a 15-day analysis, only 50% of females exhibit one complete cycle versus 95% in control females; furthermore, only 25% exhibit two complete cycles versus 82% in control females

♀ • while females frequently show persistent diestrus, a number of females go through proestrus

delayed estrous cycle ( J:167849 )

♀ • first estrus (defined as 100% cornified cells) is significantly delayed

prolonged diestrus ( J:167849 )

♀ • acyclic females remain predominantly in persistent metestrus/diestrus

prolonged metestrus ( J:167849 )

♀ • acyclic females remain predominantly in persistent metestrus/diestrus

short proestrus ( J:167849 )

♀ • time spent in the proestrus phase is significantly shortened

reduced female fertility ( J:167849 )

♀ • when mated with wild-type male mice for 90 days, none of females (0 of 8) are able to produce 4 litters whereas 4 of 6 control females do; total number of litters per female is only 2.5 +/- 0.33 versus 3.67 +/- 0.21 in controls

decreased litter size ( J:167849 )

♀ • during 90 days of mating, females produce a significantly lower number of pups per litter relative to control females (5.6 +/- 0.6 versus 12.0 +/- 1.76)

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:167849 )

♀N • females show normal basal serum follicle stimulating hormone (FSH) levels; no differences in serum FSH levels are noted after GnRH stimulation or after ovariectomy relative to controls

decreased circulating luteinizing hormone level ( J:167849 )

♀ • females have significantly lower basal serum LH levels than controls; however, serum LH levels are relatively normal after GnRH stimulation or after ovariectomy

♀ • in an LH surge induction paradigm, LH surge levels are significantly blunted relative to controls

♀ • in cycling females, proestrus LH levels are >6-fold lower than those in controls

nervous system

nervous system phenotype ( J:167849 )

N • mice show no differences in total gonadotropin-releasing hormone (GnRH) neuron number or GnRH cell body distribution across the hypothalamus relative to controls

abnormal neuron physiology ( J:167849 )

• GnRH neuronal activation is significantly attenuated during LH surge induction, as assessed by c-Fos labeling

• however, activation of GnRH neurons after release from estrogen-negative feedback is retained

endocrine/exocrine glands

decreased corpora lutea number ( J:167849 )

♀ • number of corpora lutea is severely decreased at 4 months of age

decreased ovary weight ( J:167849 )

♀ • ovary weight is significantly decreased at 4 months of age