Analysis Tools|
Allele Symbol Allele Name Allele ID |
Tg(CAG-Bgeo/GFP)21Lbe transgene insertion 21, Corrinne Lobe MGI:3046177 |
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| Summary |
19 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• small autonomous loss
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• delayed retinal ganglion cell differentiation
• however, adult mice exhibit normal proportion of retinal ganglion cells
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• small autonomous loss
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased retinal apoptosis at E12.5 to E16.5
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• cell autonomous phenotype
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• cell nonautonomous phenotype
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• at E12.5 and E13.5, retinal progenitor cells exhibit a block to cell cycle progression and retinal ganglion cell differentiation
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• cell autonomous phenotype
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• cell nonautonomous phenotype
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• at E12.5 and E13.5, retinal progenitor cells exhibit a block to cell cycle progression
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• increased retinal apoptosis at E12.5 to E16.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• epithelial to mesenchymal transition results from bleomycin-mediated injury of the lung
• only 1.4% of GFP+, epithelium derived cells express mesenchyme markers 17 days after bleomycin injury compared to 7.0% in controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• olfactory sensory neurons expressing the null allele of Nrp1 have an altered and variable pattern of glomeruli formation in the olfactory bulb, including ectopic glomeruli, but the labeled glomeruli do not show an anterior shift relative to Nrp1 wild type controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• subventricular neural stem cells treated in culture with adenoviral cre exhibit decreased differentiation compared to wild-type cells
• under differentiation condition, cultured subventricular zone cells treated with adenovirus cre produce 2-fold more oligodendrocytes and 50% more astrocytes than similarly treated wild-type cells
• however, proliferation and apoptosis rates are normal
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• under differentiation condition, cultured subventricular zone cells treated with adenovirus cre produce 3-fold fewer Tuj1+ (marking neuron precursor cells) compared similarly treated wild-type cells
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• subventricular neural stem cells treated in culture with adenoviral cre exhibit decreased differentiation compared to wild-type cells
• under differentiation condition, cultured subventricular zone cells treated with adenovirus cre produce 2-fold more oligodendrocytes and 50% more astrocytes than similarly treated wild-type cells
• however, proliferation and apoptosis rates are normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• 8-week-old male mutants do not show differences in body weight, daily food intake, ad libitum-fed blood glucose levels, or overnight fasted blood glucose levels
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• 8-week-old males display impaired glucose tolerance (elevated blood glucose after injection of glucose at 2 g/kg body weight) compared to control littermates
• no alterations in pancreatic beta cells is observed due to ectopic cre expression resulting in mutant Kcnj11 expression in beta cells
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• only about 14% of Pmch neurons show spontaneous action potentials at 5 mM glucose in brain slice preparations compared to 30% of neurons in wild-type preparations, indicating disrupted glucose-sensing
• membrane resistance of Pmch neurons is significantly reduced relative to wild-type neurons at 5 mM glucose
• almost all neurons do not hyperpolarize when extracellular glucose is reduced from 5 to 1 mM whereas around 70% of control neurons hyperpolarize under these conditions, indicating disrupted glucose-sensing
• glucose-induced depolarization is abrogated in Pmch neurons, but is readily observed in control Pmch neurons, indicating disrupted glucose-sensing
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• 8-week-old male mutants do not show differences in body weight or daily food intake
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• ad libitum-fed and overnight fasted blood glucose levels of 8-week-old male mutants are significantly reduced relative to controls
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• during glucose tolerance tests, insulin levels are decreased relative to controls, suggesting that decreased glucose levels are not the result of increased insulin secretion
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• 8-week-old males display improved glucose tolerance (after injection of glucose at 2 g/kg body weight), with a significantly reduced rise in blood glucose levels relative to controls
• no alterations in pancreatic beta cells is observed due to ectopic cre expression resulting in Ucp2 deletion in beta cells
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• about 44% of Pmch neurons fire spontaneously at 5 mM glucose, increased from around 30% of control neurons
• membrane potential of Pmch neurons displays enhanced glucose-induced depolarization in response to different glucose concentrations (left-shifted dose-response curve to glucose)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• loss of 95% of Barhl2 lineage cells
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• loss of 95% of Barhl2 lineage cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• when fed a high fat diet, mice exhibit a slight increase in fat stores
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• when fed a high fat diet, mice exhibit a slight increase in food intake
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• when fed a high fat diet, mice exhibit a slight increase in fat stores
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• mice exhibit a greater increase in weight when fed a high fat diet compared to wild-type mice
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• after a 24 hour fast, mice exhibit decreased blood glucose levels (males, 55+/-3 compared to 68+/-5 mg/dl in wild-type mice; females 53+/-2 compared to 67+/-5 mg/dl in wild-type mice)
• acute administration of insulin induces hypoglycemia
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• mice exhibit impaired fasting-mediated increase in pancreatic hormone glucagon
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• following hypoglycemia, mice fail to exhibit an increase in plasma epinephrine levels
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• during fasting and following hypoglycemia, mice fail to exhibit an increase in plasma glucagons compared to wild-type mice
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• glucose plasma levels fail to increase following central administration of 2-deoxyglucose as in wild-type mice
• however, the hyperhagic response initiated by central administration of 2-deoxyglucose is normal
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• neurons do not possess any glutamatergic excitatory postsynaptic current
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in the superficial laminae of spinal cord, there are significantly fewer lacZ-marked neurons compared to controls; significant loss of cell bodies and neuropil is observed
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• amacrine cells retain two processes after reaching the inner plexiform layer unlike unipolar wild-type cells
• amacrine cells fail to retract trailing processes upon reaching the inner plexiform layer
• amacrine cells develop a second arbor that extends to the outer misplaced plexiform layer
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• amacrine cells retain two processes after reaching the inner plexiform layer unlike unipolar wild-type cells
• amacrine cells fail to retract trailing processes upon reaching the inner plexiform layer
• amacrine cells develop a second arbor that extends to the outer misplaced plexiform layer
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• amacrine cells form two additional plexiform layers (inner and outer misplaced plexiform layers) compared with wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• melanoblasts in E14.5 skin explants show significantly reduced cell migration speed and euclidean distance travelled relative to wild-type controls
• ~30% of melanoblasts migrate with a speed slower than 0.5 um/minute versus fewer than 10% of melanoblasts in wild-type explants
• however, directionality (the ratio between the euclidean distance and total distance travelled) is normal
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• melanoblasts in E14.5 live skin explants are slightly smaller than wild type
• the rate of pseudopod generation in melanoblasts is modestly reduced but the lifetime of pseudopods is increased
• melanoblasts extend thinner protrusions with a smaller cross-sectional width than wild type controls
• thinner pseudopodia are less dynamic (longer-lived) leading to slower melanoblast migration
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• melanoblasts in E14.5 skin explants show significantly reduced cell migration speed and euclidean distance travelled relative to wild-type controls
• ~30% of melanoblasts migrate with a speed slower than 0.5 um/minute versus fewer than 10% of melanoblasts in wild-type explants
• however, directionality (the ratio between the euclidean distance and total distance travelled) is normal
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• melanoblasts in E14.5 live skin explants are slightly smaller than wild type
• the rate of pseudopod generation in melanoblasts is modestly reduced but the lifetime of pseudopods is increased
• melanoblasts extend thinner protrusions with a smaller cross-sectional width than wild type controls
• thinner pseudopodia are less dynamic (longer-lived) leading to slower melanoblast migration
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• progressive decrease in cycling T cells is observed from second to third cell division in vitro, along with large number of nonblast T cells that appear to fail to enter cell cycle
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• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase
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• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase
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• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• energy homeostasis and glucose handling are normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at P0, no Bhlhe22-expression fibers are detected in the medullary pyramidal tract unlike in wild-type mice
• in adults, few Bhlhe22-expression fibers are detected in the medullary pyramidal tract unlike in wild-type mice
• Bhlhe22-expression fibers present in the decussation do not enter the ventral dorsal funiculus of the spinal cord unlike in wild-type mice
• fewer than normal subcerebral projection neurons enter the medullary pyramidal tract while caudal corticospinal motor neurons fail to enter the tract
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• number of Shox2-expressing V2a interneurons is reduced by 98%; total number of Shox2-expressing interneurons is reduced by >80%
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• variability in locomotor parameters (cycle period, burst duration, amplitude) that are observed in isolated neonatal spinal cords exposed to low NMDA concentrations is increased in mutants compared to controls; higher NMDA concentrations reduce this variability
• other parameters such as burst frequency and flexor-extensor alternation are not significantly different
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• synaptic contacts appear to be normal for neurons arising in hippocampus from radial glial cells after treatment with tamoxifen
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice die within the first few days after birth
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| N |
• mice do not exhibit endocrine-to-acinar fate conversion
• beta cells exhibit normal cell proliferation and apoptosis
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• beta cell differentiation is impaired with insulin+ cells that are polyhormonal and ectopically express alpha cell markers unlike in control cells
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• due to reduced differentiation without an effect on proliferation and apoptosis
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• beta cell differentiation is impaired with insulin+ cells that are polyhormonal and ectopically express alpha cell markers unlike in control cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice die following E10.5 and are completely absorbed by E11.5
• however, treatment with a calcium channel agonist, 1 umol/L (-)-BayK 8644, partially rescues embryonic lethality with mice surviving until the end of E11.5
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• at E10.5, a stenosis is frequently observed between the emerging right ventricle and the outflow tract
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• at E10.5, mice display abnormal heart looping
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• at E10.5, mice display abnormal heart looping with amorphous heart tubes lying distorted within the pericardial cavity, and a stenosis is frequently observed between the emerging right ventricle and the outflow tract
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• at E10.5, pericardial effusion is associated with hemorrhages
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• at E10.5, hearts only exhibit barely noticeable tremors without any noticeable ejection volume
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• at E10.5, pericardial effusion is associated with hemorrhages
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• 63+/-2.88 beats per minute compared to 120+/-3.15 beats per minute in wild-type mice
• however, no difference in heart rate is observed at E9.5
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• at E10.5, L-type calcium channel activity and calcium-induced calcium release is impaired resulting in diminished heart pumping
• calcium channel current density is reduced compared to in wild-type mice
• unlike wild-type cardiomyocytes, E9.5 cardiomyocytes fail to respond to catecholamine treatment
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• unlike in wild-type mice, embryos size does not double between E9.5 and E10.5
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• at E9.5, vascular remodeling of the primary capillary plexus fails to occur and a honeycomb-like network of tubules with enlarged and uniform diameters forms in the extra- and intraembryonic vasculature
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• between E9.5 and E10.5
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• between E9.5 and E10.5, mice exhibit poor growth of facial primordial tissue
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• unlike in wild-type mice, embryos size does not double between E9.5 and E10.5
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• between E9.5 and E10.5, mice exhibit poor growth of facial primordial tissue
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• at E10.5, pericardial effusion is associated with hemorrhages
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• very few hemangioblasts are found in the blood vessels of the yolk sac
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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