All Phenotypes Tg(Ttr-cre/Esr1*)1Vco MGI Mouse

Phenotypes associated with this allele

Allelic Composition	Apc^tm2.1Cip/Apc^tm2.1Cip Tg(Ttr-cre/Esr1*)1Vco/0
Genetic Background	B6.Cg-Apc^tm2.1Cip Tg(Ttr-cre/Esr1*)1Vco

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apc^tm2.1Cip mutation (2 available); any Apc mutation (154 available)
Tg(Ttr-cre/Esr1*)1Vco mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased NK T cell number ( J:184496 )

• tamoxifen-treated mutants exhibit a decrease (14.9% vs. 28.6% in controls) of invariant NKT (iNKT) cells in the entire population of immune cells; the diminished proportion of iNKTs is more pronounced in the CD4+ iNKT cells than in the CD4- iNKT cells

abnormal NK T cell physiology ( J:184496 )

• iNKT activation level is higher in the liver of tamoxifen-treated mutants than in controls

immune system

decreased NK T cell number ( J:184496 )

abnormal NK T cell physiology ( J:184496 )

• iNKT activation level is higher in the liver of tamoxifen-treated mutants than in controls

increased interferon-gamma secretion ( J:184496 )

• CD4+ and CD4- subpopulations of iNKTs isolated from mutant livers produce higher amounts of IFN-gamma compared to controls

increased interleukin-4 secretion ( J:184496 )

• CD4+ and CD4- subpopulations of iNKTs isolated from mutant livers produce higher amounts of IL-4 compared to controls

liver inflammation ( J:184496 )

• tamoxifen-treated mutants exhibit higher absolute numbers of most types of immune cells in the liver, including Kupffer cells, granulocytes, NK, B lymphocytes, and conventional T cells

• mutant livers are more sensitive to ConA-induced hepatitis than controls

liver/biliary system

liver inflammation ( J:184496 )

• tamoxifen-treated mutants exhibit higher absolute numbers of most types of immune cells in the liver, including Kupffer cells, granulocytes, NK, B lymphocytes, and conventional T cells

• mutant livers are more sensitive to ConA-induced hepatitis than controls

abnormal liver morphology ( J:184496 )

• mutants injected with tamoxifen exhibit significantly higher total numbers of nonparenchymal cells (NPCs) in the liver

enlarged liver ( J:184496 )

• mutants injected with tamoxifen develop progressive hepatomegaly

growth/size/body

enlarged liver ( J:184496 )

• mutants injected with tamoxifen develop progressive hepatomegaly

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:184496 )

• tamoxifen treated mutants exhibit a decrease in survival rate

liver/biliary system

increased hepatocyte apoptosis ( J:184496 )

• increase in liver apoptosis in tamoxifen treated mutants

liver inflammation ( J:184496 )

• liver inflammation is increased in tamoxifen treated mutants compared to single Apc mutants

abnormal liver morphology ( J:184496 )

• livers of tamoxifen treated mutants exhibit a flaccid and loose texture compared to livers from single Apc homozygotes

• mutants injected with tamoxifen exhibit significantly higher total numbers of nonparenchymal cells (NPCs) in the liver

hepatic necrosis ( J:184496 )

• in tamoxifen treated mutants

immune system

increased NK T cell number ( J:184496 )

• number of iNKT cells in the liver is increased in tamoxifen-treated double mutants compared to single Apc mutants

abnormal neutrophil physiology ( J:184496 )

• liver infiltrating immune cells of tamoxifen treated mutants have an increase in neutrophils compared to infiltrating immune cells in single Apc mutants

abnormal NK T cell physiology ( J:184496 )

• invariant NKTs (iNKTs) are more activated in tamoxifen treated double mutant livers than in single Apc mutant livers

abnormal chemokine level ( J:184496 )

• chemokines in the liver of tamoxifen treated mutants, such as Cxcl1, Cxcl12, and Cxcl12 are induced to higher levels in double mutants than in single Apc mutants

decreased interferon-gamma secretion ( J:184496 )

• CD4- iNKTs from tamoxifen treated mutants produce less IFN-gamma than CD4- iNKTs of single Apc mutants

increased interleukin-4 secretion ( J:184496 )

• CD4- iNKTs from tamoxifen treated mutants produce more IL-4 than CD4- iNKTs of single Apc mutants

liver inflammation ( J:184496 )

• liver inflammation is increased in tamoxifen treated mutants compared to single Apc mutants

homeostasis/metabolism

abnormal circulating enzyme level ( J:184496 )

• serum transaminase levels are higher in tamoxifen treated mutants than in single Apc homozygotes

abnormal chemokine level ( J:184496 )

• chemokines in the liver of tamoxifen treated mutants, such as Cxcl1, Cxcl12, and Cxcl12 are induced to higher levels in double mutants than in single Apc mutants

hematopoietic system

increased NK T cell number ( J:184496 )

• number of iNKT cells in the liver is increased in tamoxifen-treated double mutants compared to single Apc mutants

abnormal neutrophil physiology ( J:184496 )

• liver infiltrating immune cells of tamoxifen treated mutants have an increase in neutrophils compared to infiltrating immune cells in single Apc mutants

abnormal NK T cell physiology ( J:184496 )

• invariant NKTs (iNKTs) are more activated in tamoxifen treated double mutant livers than in single Apc mutant livers

cellular

increased hepatocyte apoptosis ( J:184496 )

• increase in liver apoptosis in tamoxifen treated mutants

Allelic Composition	Anapc2^tm1Kna/Anapc2^tm1Kna Tg(Ttr-cre/Esr1*)1Vco/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Anapc2^tm1Kna mutation (0 available); any Anapc2 mutation (36 available)
Tg(Ttr-cre/Esr1*)1Vco mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:87782 )

• 40% of mutants injected with 4-hydroxytamoxifen (4-OHT) to delete Anapc2 specifically in hepatocytes, die of liver failure within 2-3 weeks of the first injection

• surviving mutants subjected to 1/4 hepatectomy one month after the first 4-OHT injection, die from liver failure between 12-15 days after the hepatectomy, whereas controls survive

liver/biliary system

increased hepatocyte proliferation ( J:87782 )

• quiescent hepatocytes spontaneously enter the cell cycle and proliferate and arrest in a mitotic-like state

abnormal hepatocyte morphology ( J:87782 )

• hepatocytes are enlarged and arrested in metaphase in mutants treated with 4-OHT to delete Anapc2 in hepatocytes

liver failure ( J:87782 )

• 40% of mutants injected with 4-OHT to delete Anapc2 specifically in hepatocytes, die of liver failure within 2-3 weeks of the first injection

cellular

increased hepatocyte proliferation ( J:87782 )

• quiescent hepatocytes spontaneously enter the cell cycle and proliferate and arrest in a mitotic-like state

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