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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Chek1tm1Jmr
targeted mutation 1, Jeffrey M Rosen
MGI:3046694
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Chek1tm1Jmr/Chek1tm1Jmr
Tg(Wap-cre)11738Mam/0 		involves: C57BL/6 MGI:3046696
cn2
 		Chek1tm1Jmr/Chek1+
Tg(Wap-cre)11738Mam/0 		involves: C57BL/6 MGI:3046697
cn3
 		Chek1tm1Jmr/Chek1tm1Jmr
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y 		involves: C57BL/6 * CBA * DBA/2 MGI:6220868
cn4
 		Chek1tm1Jmr/Chek1+
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y 		involves: C57BL/6 * CBA * DBA/2 MGI:6220869
cn5
 		Chek1tm1Jmr/Chek1tm1Jmr
Tg(Tyr-cre)1Lru/Y 		involves: C57BL/6 * DBA/2 MGI:6220867


Genotype
MGI:3046696
cn1
Allelic
Composition		 Chek1tm1Jmr/Chek1tm1Jmr
Tg(Wap-cre)11738Mam/0
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Wap-cre)11738Mam mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal apoptosis ( J:91266 )
• about 10% - 12% is seen in the differentiating lobuloalveolar mammary epithelial cells compared to less than 1% in wild-type
decreased cell proliferation ( J:91266 )
• about 4% of cells in the mammary glands are proliferating at day 1 of lactation compared to 6.5% in wild-type

endocrine/exocrine glands
abnormal mammary gland growth during pregnancy ( J:91266 )
• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
abnormal lactation ( J:91266 )
• females fail to nurse their young

reproductive system
abnormal mammary gland growth during pregnancy ( J:91266 )
• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre

integument
abnormal mammary gland growth during pregnancy ( J:91266 )
• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
abnormal lactation ( J:91266 )
• females fail to nurse their young




Genotype
MGI:3046697
cn2
Allelic
Composition		 Chek1tm1Jmr/Chek1+
Tg(Wap-cre)11738Mam/0
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Wap-cre)11738Mam mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
increased cell proliferation ( J:91266 )
• about 35% of cells in the mammary glands are proliferating at day 1 of lactation compared to 6.5% in wild-type




Genotype
MGI:6220868
cn3
Allelic
Composition		 Chek1tm1Jmr/Chek1tm1Jmr
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y
Genetic
Background		 involves: C57BL/6 * CBA * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Dct-lacZ)A12Jkn mutation (5 available)
Tg(Tyr-cre)1Lru mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
embryo
abnormal melanoblast morphology ( J:265850 )
• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes
• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis
• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5

nervous system
abnormal melanoblast morphology ( J:265850 )
• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes
• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis
• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5

cellular
abnormal DNA replication ( J:265850 )
• many gamma-H2AX+ melanoblasts are noted in the head and trunk regions, unlike in control embryos, suggesting that aberrant DNA replication leads to spontaneous DNA damage and ultimately cell death

homeostasis/metabolism
abnormal DNA replication ( J:265850 )
• many gamma-H2AX+ melanoblasts are noted in the head and trunk regions, unlike in control embryos, suggesting that aberrant DNA replication leads to spontaneous DNA damage and ultimately cell death




Genotype
MGI:6220869
cn4
Allelic
Composition		 Chek1tm1Jmr/Chek1+
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y
Genetic
Background		 involves: C57BL/6 * CBA * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Dct-lacZ)A12Jkn mutation (5 available)
Tg(Tyr-cre)1Lru mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
pigmentation phenotype ( J:265850 )
N
• despite a slight reduction of melanoblast numbers at E13.5, adult mice exhibit normal coat pigmentation relative to control mice

embryo
abnormal melanoblast morphology ( J:265850 )
• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos

nervous system
abnormal melanoblast morphology ( J:265850 )
• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos




Genotype
MGI:6220867
cn5
Allelic
Composition		 Chek1tm1Jmr/Chek1tm1Jmr
Tg(Tyr-cre)1Lru/Y
Genetic
Background		 involves: C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Tyr-cre)1Lru mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
abnormal hair follicle melanocyte morphology ( J:265850 )
• no DCT-expressing melanocytes are observed in the hair follicles, unlike in control mice
absent coat pigmentation ( J:265850 )
• adult male mice are completely devoid of coat pigmentation, unlike control mice
abnormal epidermal melanocyte morphology ( J:265850 )
• male mice show complete lack of epidermal melanocytes
decreased melanocyte number ( J:265850 )
• adult males show a marked reduction or absence of neural crest-derived melanocytes in the eyes, unlike control mice
• however, males retain normal eye pigmentation in the retinal pigmented epithelium (RPE) due to inefficient transgene expression in the RPE

integument
abnormal hair follicle melanocyte morphology ( J:265850 )
• no DCT-expressing melanocytes are observed in the hair follicles, unlike in control mice
absent coat pigmentation ( J:265850 )
• adult male mice are completely devoid of coat pigmentation, unlike control mice
abnormal epidermal melanocyte morphology ( J:265850 )
• male mice show complete lack of epidermal melanocytes




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Send questions and comments to User Support. 		last database update
10/22/2024
MGI 6.24 		The Jackson Laboratory