About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Chek1tm1Jmr
targeted mutation 1, Jeffrey M Rosen
MGI:3046694
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Chek1tm1Jmr/Chek1tm1Jmr
Tg(Wap-cre)11738Mam/0
involves: C57BL/6 MGI:3046696
cn2
Chek1tm1Jmr/Chek1+
Tg(Wap-cre)11738Mam/0
involves: C57BL/6 MGI:3046697
cn3
Chek1tm1Jmr/Chek1tm1Jmr
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y
involves: C57BL/6 * CBA * DBA/2 MGI:6220868
cn4
Chek1tm1Jmr/Chek1+
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y
involves: C57BL/6 * CBA * DBA/2 MGI:6220869
cn5
Chek1tm1Jmr/Chek1tm1Jmr
Tg(Tyr-cre)1Lru/Y
involves: C57BL/6 * DBA/2 MGI:6220867


Genotype
MGI:3046696
cn1
Allelic
Composition
Chek1tm1Jmr/Chek1tm1Jmr
Tg(Wap-cre)11738Mam/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Wap-cre)11738Mam mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• about 10% - 12% is seen in the differentiating lobuloalveolar mammary epithelial cells compared to less than 1% in wild-type
• about 4% of cells in the mammary glands are proliferating at day 1 of lactation compared to 6.5% in wild-type

endocrine/exocrine glands
• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
• females fail to nurse their young

reproductive system
• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre

integument
• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
• females fail to nurse their young




Genotype
MGI:3046697
cn2
Allelic
Composition
Chek1tm1Jmr/Chek1+
Tg(Wap-cre)11738Mam/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Wap-cre)11738Mam mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• about 35% of cells in the mammary glands are proliferating at day 1 of lactation compared to 6.5% in wild-type




Genotype
MGI:6220868
cn3
Allelic
Composition
Chek1tm1Jmr/Chek1tm1Jmr
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y
Genetic
Background
involves: C57BL/6 * CBA * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Dct-lacZ)A12Jkn mutation (5 available)
Tg(Tyr-cre)1Lru mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes
• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis
• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5

nervous system
• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes
• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis
• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5

cellular
• many gamma-H2AX+ melanoblasts are noted in the head and trunk regions, unlike in control embryos, suggesting that aberrant DNA replication leads to spontaneous DNA damage and ultimately cell death

homeostasis/metabolism
• many gamma-H2AX+ melanoblasts are noted in the head and trunk regions, unlike in control embryos, suggesting that aberrant DNA replication leads to spontaneous DNA damage and ultimately cell death




Genotype
MGI:6220869
cn4
Allelic
Composition
Chek1tm1Jmr/Chek1+
Tg(Dct-lacZ)A12Jkn/0
Tg(Tyr-cre)1Lru/Y
Genetic
Background
involves: C57BL/6 * CBA * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Dct-lacZ)A12Jkn mutation (5 available)
Tg(Tyr-cre)1Lru mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
pigmentation
N
• despite a slight reduction of melanoblast numbers at E13.5, adult mice exhibit normal coat pigmentation relative to control mice

embryo
• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos

nervous system
• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos




Genotype
MGI:6220867
cn5
Allelic
Composition
Chek1tm1Jmr/Chek1tm1Jmr
Tg(Tyr-cre)1Lru/Y
Genetic
Background
involves: C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Tyr-cre)1Lru mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
pigmentation
• no DCT-expressing melanocytes are observed in the hair follicles, unlike in control mice
• adult male mice are completely devoid of coat pigmentation, unlike control mice
• male mice show complete lack of epidermal melanocytes
• adult males show a marked reduction or absence of neural crest-derived melanocytes in the eyes, unlike control mice
• however, males retain normal eye pigmentation in the retinal pigmented epithelium (RPE) due to inefficient transgene expression in the RPE

integument
• no DCT-expressing melanocytes are observed in the hair follicles, unlike in control mice
• adult male mice are completely devoid of coat pigmentation, unlike control mice
• male mice show complete lack of epidermal melanocytes





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/10/2024
MGI 6.24
The Jackson Laboratory