All Phenotypes Tbk1<tm1Aki> MGI Mouse

mortality/aging ( J:132662 )

N	• the Tnf null background prevents the lethality that occurs at E14.5 for Tbk1 homozygotes

decreased IgG level ( J:132662 )

• serum titers of antigen specific IgG in response to a DNA vaccine are almost 4-log lower than those in control

• when mice serve as bone marrow donors to irradiated control mice, similar defects in antigen specific IgG production after immunization are observed

decreased IgG1 level ( J:132662 )

• serum titers of antigen specific IgG1 in response to a DNA vaccine are significantly less

decreased IgG2a level ( J:132662 )

• serum titers of antigen specific IgG2a in response to a DNA vaccine are significantly les

• when mice serve as bone marrow donors to irradiated control mice, similar defects in antigen specific IgG2a production after immunization are observed

abnormal cytotoxic T cell physiology ( J:132662 )

• there is a 10-fold reduction in the number of antigen specific CD8 T cells two weeks after immunization with a DNA vaccine compared to vaccinated controls

• when mice are irradiated and receive wild-type bone marrow, CD8 T cells proliferate at a significantly reduced level upon exposure to antigen encoded in the DNA vaccine

decreased cytotoxic T cell cytolysis ( J:132662 )

• there is a reduction in antigen specific CTL activity two weeks after immunization with a DNA vaccine compared to vaccinated controls

decreased interferon-gamma secretion ( J:132662 )

• splenocytes from mice immunized with a DNA vaccine produce significantly less IFN-gamma upon stimulation with antigen in culture compared to vaccinated controls

• when mice serve as bone marrow donors to irradiated control mice, similar defects in IFN-gamma secretion after immunization are observed