Analysis Tools
mortality/aging
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• homozygous mutants die shortly after birth
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cellular
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• massive cell death is seen in the core mesenchyme of the limb buds by E11.0
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• massive cell death is seen in the metanephric mesenchyme by E11.0
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homeostasis/metabolism
limbs/digits/tail
oligodactyly
(
J:90991
)
|
• the number of digits is reduced and posterior identity and soft tissue webbing are lost
|
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• the ulna and radius fuse during the onset of ossification
• only one skeletal element forms in hind limbs
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• metacarpal fusions are seen
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• the zeugopods are abnormal and the autopods are truncated
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• the apical ectodermal ridge morphology is disrupted
|
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• massive cell death is seen in the core mesenchyme of the limb buds by E11.0
|
renal/urinary system
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• failure to induce condensation of the metanephric mesenchyme
|
|
• massive cell death is seen in the metanephric mesenchyme by E11.0
|
absent kidney
(
J:90991
)
|
• bilateral renal agenesis is seen at birth; however, gonads and adrenal glands develop normally
• the kidney phenotype is fully penetrant on a 129S or CD1 background
|
absent ureter
(
J:90991
)
|
• bilateral agenesis of the ureters is seen at birth
|
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• the neonatal bladder is not filled
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• the ureteric bud does not branch at E11.5
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• initiation of ureteric bud growth is completely blocked
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• invasion of the metanephric mesenchyme by the ureteric bud fails to occur
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respiratory system
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• the airway epithelium remains multi-layered unlike in wild-type embryos
|
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• the number of differentiated alveoli are reduced
|
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• neonatal dyspnea
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skeleton
|
• the ulna and radius fuse during the onset of ossification
• only one skeletal element forms in hind limbs
|
|
• metacarpal fusions are seen
|
embryo
|
• massive cell death is seen in the metanephric mesenchyme by E11.0
|
|
• the apical ectodermal ridge morphology is disrupted
|
|
• massive cell death is seen in the core mesenchyme of the limb buds by E11.0
|
