Phenotypes associated with this allele

• Allele Symbol: Zdhhc8^tm1Kara
• Allele Name: targeted mutation 1, Maria Karayiorgou
• Allele ID: MGI:3047084
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zdhhc8^tm1Kara/Zdhhc8^tm1Kara	involves: 129/Sv * C57BL/6	MGI:3047390
hm2	Zdhhc8^tm1Kara/Zdhhc8^tm1Kara	Not Specified	MGI:5766949
ht3	Zdhhc8^tm1Kara/Zdhhc8^+	involves: 129/Sv * C57BL/6	MGI:3047393
ht4	Zdhhc8^tm1Kara/Zdhhc8^+	Not Specified	MGI:5766950

Genotype
MGI:3047390

hm1

• Allelic Composition: Zdhhc8^tm1Kara/Zdhhc8^tm1Kara
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

decreased exploration in new environment ( J:91131 )

• homozygous females travel and rear significantly less and avoid the center more in an open field test

• homozygous males show a milder decrease in locomotor activity in an open field test

nervous system

decreased prepulse inhibition ( J:91131 )

• homozygous female but not male mutants display a significant reduction in prepulse inhibition compared to wild-type littermates at 78 db

Genotype
MGI:5766949

hm2

• Allelic Composition: Zdhhc8^tm1Kara/Zdhhc8^tm1Kara
• Genetic Background: Not Specified

nervous system

abnormal dendrite morphology ( J:141432 )

• reduction in dendritic complexity in primary cultured neurons, with hippocampal neurons exhibiting a 19.5% decrease in the number of primary dendrites and a 41% decrease in the number of branch points

• CA1 pyramidal neurons show a simplification in the dendritic arbors, with 8 week old mice showing a 21% decrease in the total number of branch points in the basal dendritic tree of CA1 neurons

abnormal dendritic spine morphology ( J:141432 )

• the number of mushroom spines in primary cultured neurons is reduced by 43%

• reduction in the mushroom spine density in CA1 pyramidal neurons

• however, no changes in the width and length of primary culture neurons and CA1 pyramidal spines are seen

abnormal synapse morphology ( J:141432 )

• reduction in the density of glutamatergic synaptic contacts in vitro and in vivo

Genotype
MGI:3047393

ht3

• Allelic Composition: Zdhhc8^tm1Kara/Zdhhc8⁺
• Genetic Background: involves: 129/Sv * C57BL/6

behavior/neurological

decreased exploration in new environment ( J:91131 )

• heterozygous females travel and rear significantly less and avoid the center more in an open field test

Genotype
MGI:5766950

ht4

• Allelic Composition: Zdhhc8^tm1Kara/Zdhhc8⁺
• Genetic Background: Not Specified

nervous system

abnormal dendrite morphology ( J:141432 )

• reduction in dendritic complexity in primary cultured neurons, with hippocampal neurons exhibiting a 12% decrease in the number of primary dendrites and a 37% decrease in the number of branch points

• CA1 pyramidal neurons show a simplification in the dendritic arbors, with 8 week old mice showing a 19% decrease in the total number of branch points in the basal dendritic tree of CA1 neurons

abnormal dendritic spine morphology ( J:141432 )

• the number of mushroom spines in mutant neurons is reduced by 40%

• reduction in the mushroom spine density in CA1 pyramidal neurons

• however, no changes in the width and length of primary cultured neurons and CA1 pyramidal spines are seen

abnormal synapse morphology ( J:141432 )

• reduction in the density of glutamatergic synaptic contacts