Phenotypes associated with this allele

• Allele Symbol: Tg(Wnt1-GAL4)1Rth
• Allele Name: transgene insertion 1, David H Rowitch
• Allele ID: MGI:3047162
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Hprt1^tm1(UAS-Bmp4)Bhr/Hprt1^+ Tg(Wnt1-GAL4)1Rth/0	involves: 129S7/SvEvBrd * C57BL/6	MGI:3047259
cx2	Hprt1^tm2(UAS-Bmp4)Bhr/Hprt1^+ Tg(Wnt1-GAL4)1Rth/0	involves: 129S7/SvEvBrd * C57BL/6	MGI:3047260
cx3	Tg(UAS-Shh)1Rth/0 Tg(Wnt1-GAL4)1Rth/0	involves: C57BL/6J * CBA/J	MGI:3047247

Genotype
MGI:3047259

cx1

• Allelic Composition: Hprt1^{tm1(UAS-Bmp4)Bhr}/Hprt1⁺; Tg(Wnt1-GAL4)1Rth/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:91296 )

♀ • many transgenic embryos die between E11.5 and E12.5

cardiovascular system

hemorrhage ( J:91296 )

♀ • at E11.5 embryos display midbrain hemorrhage

growth/size/body

embryonic growth retardation ( J:91296 )

♀ • by E11.5 embryos are growth retarded

nervous system

exencephaly ( J:91296 )

♀ • at E11.5 embryos display midbrain exencephaly

embryo

embryonic growth retardation ( J:91296 )

♀ • by E11.5 embryos are growth retarded

Genotype
MGI:3047260

cx2

• Allelic Composition: Hprt1^{tm2(UAS-Bmp4)Bhr}/Hprt1⁺; Tg(Wnt1-GAL4)1Rth/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:91296 )

♀ • many transgenic embryos die between E11.5 and E12.5

cardiovascular system

abnormal blood vessel morphology ( J:91296 )

♀ • at E11.5 embryos display ectopic blood vessel formation

hemorrhage ( J:91296 )

♀ • at E11.5 embryos display midbrain hemorrhage

growth/size/body

embryonic growth retardation ( J:91296 )

♀ • by E11.5 embryos are growth retarded

vision/eye

abnormal eye morphology ( J:91296 )

♀ • at E11.5 eye abnormalities are seen

nervous system

exencephaly ( J:91296 )

♀ • at E11.5 embryos display midbrain exencephaly

embryo

embryonic growth retardation ( J:91296 )

♀ • by E11.5 embryos are growth retarded

Genotype
MGI:3047247

cx3

• Allelic Composition: Tg(UAS-Shh)1Rth/0; Tg(Wnt1-GAL4)1Rth/0
• Genetic Background: involves: C57BL/6J * CBA/J

cardiovascular system

abnormal blood vessel morphology ( J:57948 )

• there is a pronounced blood supply to the dorsal spinal cord

hemorrhage ( J:57948 )

• hemorrhage may be seen around the dorsal spinal cord

cellular

increased cell proliferation ( J:57948 )

• increased proliferation is seen in the spinal cord at E12.5 but not at E18.5

craniofacial

abnormal neurocranium morphology ( J:57948 )

• the membranous skull is absent

muscle

abnormal epaxial muscle morphology ( J:57948 )

• the epiaxial muscles that normally overlay the brain and spinal cord are absent

skeleton

abnormal neurocranium morphology ( J:57948 )

• the membranous skull is absent

absent vertebral arch ( J:57948 )

• the neural arches are absent

nervous system

abnormal midbrain morphology ( J:57948 )

• ventralization of the midbrain is seen by E9.5

midbrain hyperplasia ( J:57948 )

• neural hyperplasia is first detected at E9.5

• at E18.5 a tissue mass is seen emanating from the midbrain and spinal cord with a folded appearance protruding out the back of the mutants

abnormal spinal cord morphology ( J:57948 )

• at E12.5 dorsal-ventral organization of the neural precursor cells in the spinal cord is absent

hydromyelia ( J:57948 )

• between E12.5 and E18.5 expansion of the ventricular zone is seen

increased spinal cord size ( J:57948 )

• at E18.5 a tissue mass, with a folded appearance, is seen emanating from the midbrain and spinal cord and protruding out the back of the mutants

• there is a pronounced blood supply to the dorsal spinal cord

homeostasis/metabolism

hydromyelia ( J:57948 )

• between E12.5 and E18.5 expansion of the ventricular zone is seen