Phenotypes associated with this allele

• Allele Symbol: Klf2^tm1Ling
• Allele Name: targeted mutation 1, Jerry B Lingrel
• Allele ID: MGI:3047647
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Klf2^tm1Ling/Klf2^tm1Ling	involves: 129P2/OlaHsd	MGI:3765434
hm2	Klf2^tm1Ling/Klf2^tm1Ling	involves: 129P2/OlaHsd * C57BL/6J	MGI:3047734

Genotype
MGI:3765434

hm1

• Allelic Composition: Klf2^tm1Ling/Klf2^tm1Ling
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:127228 )

• embryos are all dead by E14.5

Genotype
MGI:3047734

hm2

• Allelic Composition: Klf2^tm1Ling/Klf2^tm1Ling
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:51402 )

• all homozygotes are viable up to 11.5 dpc; no embryos are found alive beyond 13.5 dpc

cardiovascular system

hemorrhage ( J:51402 )

• at 11.5 dpc homozygotes are indistinguishable from their wild-type or heterozygous counterparts

• by 12.5 dpc, the majority of mutant embryos display abdominal bleeding

• notably, no hemorrhagic sites are found, and the vasculature of homozygotes appears intact

craniofacial

abnormal mandible morphology ( J:51402 )

• by 12.5 dpc, most homozygotes display a malformed lower jaw

embryo

embryonic growth retardation ( J:51402 )

• by 12.5 dpc, the majority of mutant embryos exhibit retarded growth

pale yolk sac ( J:51402 )

• by 12.5 dpc, the majority of mutant embryos exhibit a pale yolk sac

growth/size/body

embryonic growth retardation ( J:51402 )

• by 12.5 dpc, the majority of mutant embryos exhibit retarded growth

hematopoietic system

anemia ( J:51402 )

• by 12.5 dpc, the majority of mutant embryos exhibit severe anemia; the amount of peripheral blood is significantly reduced

impaired hematopoiesis ( J:51402 )

• at 11.5 dpc, homozygotes show a siginificant reduction in fetal liver hematopoiesis

increased nucleated erythrocyte cell number ( J:51402 )

• cytospin preparations of blood smears from mutant embryos show only large nucleated red cells of primitive origin, as opposed to the mature non-nucleated red blood cells found in wild-type

immune system

immune system phenotype ( J:51402 )

N • in vitro fetal liver cultures from mutant embryos indicated normal macrophage development

liver/biliary system

pale liver ( J:51402 )

• by 12.5 dpc, mutant livers show a significant reduction in the hematopoietic precursors and an overall pallor

• also, mutant fetal livers exhibit a severe reduction in CFU-E formation, depite normal yolk sac erythropoiesis

respiratory system

abnormal lung morphology ( J:51402 )

• in some 11.5 dpc homozygotes, the amount of branching in the lung is slightly reduced

• all other organs appear histologically normal

skeleton

abnormal mandible morphology ( J:51402 )

• by 12.5 dpc, most homozygotes display a malformed lower jaw