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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cd274tm1Shr
targeted mutation 1, Arlene H Sharpe
MGI:3050803
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Cd274tm1Shr/Cd274tm1Shr C.B6-Cd274tm1Shr MGI:3721514
hm2
Cd274tm1Shr/Cd274tm1Shr involves: C57BL/6 MGI:3051544
cx3
Cd274tm1Shr/Cd274tm1Shr
Pdcd1lg2tm3Shr/Pdcd1lg2tm3Shr
C.B6-Cd274tm1Shr Pdcd1lg2tm3Shr MGI:3721513
cx4
Cd274tm1Shr/Cd274tm1Shr
Tg(TcraTcrb)1100Mjb/0
involves: C57BL/6 MGI:4397688


Genotype
MGI:3721514
hm1
Allelic
Composition
Cd274tm1Shr/Cd274tm1Shr
Genetic
Background
C.B6-Cd274tm1Shr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in an adoptive transfer experiment into mice, DO11 transgenic T cells are modestly stimulated to over-produce interferon-gamma
• in an adoptive transfer experiment into mice, DO11 transgenic T cells are modestly stimulated to over-produce IL-2
• in an adoptive transfer experiment into mice, IL-4+ DO11 transgenic cells are reduced by 56%
• onset of automimmune diabetes mellitus is accelerated, sex-specific disease progression is lost, and penetrance is complete similar to in Pdcd1lg2tm3Shr homozygotes




Genotype
MGI:3051544
hm2
Allelic
Composition
Cd274tm1Shr/Cd274tm1Shr
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• when stimulated with low concentrations of anti CD3, CD4+ cells produce increased amounts of IFN-gamma
• when stimulated with high concentrations of anti CD3, IFN-gamma response was comparable to controls
• IFN-gamma produced at increased levels when cells are stimulate with high levels of antibody
• at low levels of antibody cells produce undetectable levels of IFN-gamma, even in controls
• Cd8+ cell expansion is increased after stimulation with high antibody levels

immune system
• when stimulated with low concentrations of anti CD3, CD4+ cells produce increased amounts of IFN-gamma
• when stimulated with high concentrations of anti CD3, IFN-gamma response was comparable to controls
• IFN-gamma produced at increased levels when cells are stimulate with high levels of antibody
• at low levels of antibody cells produce undetectable levels of IFN-gamma, even in controls
• Cd8+ cell expansion is increased after stimulation with high antibody levels




Genotype
MGI:3721513
cx3
Allelic
Composition
Cd274tm1Shr/Cd274tm1Shr
Pdcd1lg2tm3Shr/Pdcd1lg2tm3Shr
Genetic
Background
C.B6-Cd274tm1Shr Pdcd1lg2tm3Shr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
Pdcd1lg2tm3Shr mutation (0 available); any Pdcd1lg2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• bone marrow dendritic cells increases the production of IL-2 and interferon-gamma from DO11 transgenic T cells in vitro by 300% to 400% when compared to wild-type cells
• in an in vitro assay with dendritic cell or an adoptive transfer experiment into mice, DO11 transgenic T cells are stimulated to over-produce interferon-gamma
• in an in vitro assay with dendritic cell or an adoptive transfer experiment into mice, DO11 transgenic T cells are stimulated to over-produce IL-2
• bone marrow dendritic cells fail to stimulate the production of IL-4 from DO11 T cells in vitro or in adoptive transfer experiments
• in an adoptive transfer experiment into mice, IL-4+ DO11 transgenic cells are reduced by 86%




Genotype
MGI:4397688
cx4
Allelic
Composition
Cd274tm1Shr/Cd274tm1Shr
Tg(TcraTcrb)1100Mjb/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• following adoptive transfer, T cells induce rapid onset of diabetes in RIP-ovahigh recipients within 1 to 3 weeks unlike similarly treated wild-type cells





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory