About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cd274tm1Shr
targeted mutation 1, Arlene H Sharpe
MGI:3050803
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cd274tm1Shr/Cd274tm1Shr C.B6-Cd274tm1Shr MGI:3721514
hm2
 		Cd274tm1Shr/Cd274tm1Shr involves: C57BL/6 MGI:3051544
cx3
 		Cd274tm1Shr/Cd274tm1Shr
Pdcd1lg2tm3Shr/Pdcd1lg2tm3Shr 		C.B6-Cd274tm1Shr Pdcd1lg2tm3Shr MGI:3721513
cx4
 		Cd274tm1Shr/Cd274tm1Shr
Tg(TcraTcrb)1100Mjb/0 		involves: C57BL/6 MGI:4397688


Genotype
MGI:3721514
hm1
Allelic
Composition		 Cd274tm1Shr/Cd274tm1Shr
Genetic
Background		 C.B6-Cd274tm1Shr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased interferon-gamma secretion ( J:123785 )
• in an adoptive transfer experiment into mice, DO11 transgenic T cells are modestly stimulated to over-produce interferon-gamma
abnormal interleukin secretion ( J:123785 )
• in an adoptive transfer experiment into mice, DO11 transgenic T cells are modestly stimulated to over-produce IL-2
• in an adoptive transfer experiment into mice, IL-4+ DO11 transgenic cells are reduced by 56%
increased susceptibility to autoimmune diabetes ( J:123785 )
• onset of automimmune diabetes mellitus is accelerated, sex-specific disease progression is lost, and penetrance is complete similar to in Pdcd1lg2tm3Shr homozygotes




Genotype
MGI:3051544
hm2
Allelic
Composition		 Cd274tm1Shr/Cd274tm1Shr
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal CD4-positive, alpha beta T cell morphology ( J:91480 )
• when stimulated with low concentrations of anti CD3, CD4+ cells produce increased amounts of IFN-gamma
• when stimulated with high concentrations of anti CD3, IFN-gamma response was comparable to controls
abnormal CD8-positive, alpha beta T cell morphology ( J:91480 )
• IFN-gamma produced at increased levels when cells are stimulate with high levels of antibody
• at low levels of antibody cells produce undetectable levels of IFN-gamma, even in controls
• Cd8+ cell expansion is increased after stimulation with high antibody levels

immune system
abnormal CD4-positive, alpha beta T cell morphology ( J:91480 )
• when stimulated with low concentrations of anti CD3, CD4+ cells produce increased amounts of IFN-gamma
• when stimulated with high concentrations of anti CD3, IFN-gamma response was comparable to controls
abnormal CD8-positive, alpha beta T cell morphology ( J:91480 )
• IFN-gamma produced at increased levels when cells are stimulate with high levels of antibody
• at low levels of antibody cells produce undetectable levels of IFN-gamma, even in controls
• Cd8+ cell expansion is increased after stimulation with high antibody levels




Genotype
MGI:3721513
cx3
Allelic
Composition		 Cd274tm1Shr/Cd274tm1Shr
Pdcd1lg2tm3Shr/Pdcd1lg2tm3Shr
Genetic
Background		 C.B6-Cd274tm1Shr Pdcd1lg2tm3Shr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
Pdcd1lg2tm3Shr mutation (0 available); any Pdcd1lg2 mutation (25 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal dendritic cell physiology ( J:123785 )
• bone marrow dendritic cells increases the production of IL-2 and interferon-gamma from DO11 transgenic T cells in vitro by 300% to 400% when compared to wild-type cells
increased interferon-gamma secretion ( J:123785 )
• in an in vitro assay with dendritic cell or an adoptive transfer experiment into mice, DO11 transgenic T cells are stimulated to over-produce interferon-gamma
increased interleukin-2 secretion ( J:123785 )
• in an in vitro assay with dendritic cell or an adoptive transfer experiment into mice, DO11 transgenic T cells are stimulated to over-produce IL-2
decreased interleukin-4 secretion ( J:123785 )
• bone marrow dendritic cells fail to stimulate the production of IL-4 from DO11 T cells in vitro or in adoptive transfer experiments
• in an adoptive transfer experiment into mice, IL-4+ DO11 transgenic cells are reduced by 86%




Genotype
MGI:4397688
cx4
Allelic
Composition		 Cd274tm1Shr/Cd274tm1Shr
Tg(TcraTcrb)1100Mjb/0
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd274tm1Shr mutation (2 available); any Cd274 mutation (70 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to autoimmune diabetes ( J:153040 )
• following adoptive transfer, T cells induce rapid onset of diabetes in RIP-ovahigh recipients within 1 to 3 weeks unlike similarly treated wild-type cells




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory