Phenotypes associated with this allele

• Allele Symbol: Cd274^tm2Shr
• Allele Name: targeted mutation 2, Arlene H Sharpe
• Allele ID: MGI:3050804
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd274^tm2Shr/Cd274^tm2Shr	involves: 129/Sv * 129S4/SvJae	MGI:3688404
cx2	Cd274^tm2Shr/Cd274^tm2Shr Pdcd1lg2^tm4Shr/Pdcd1lg2^tm4Shr	involves: 129S4/SvJae	MGI:4821796

Genotype
MGI:3688404

hm1

• Allelic Composition: Cd274^tm2Shr/Cd274^tm2Shr
• Genetic Background: involves: 129/Sv * 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal cytokine secretion ( J:113851 )

• upon stimulation with L. mexicana antigen for 4 days, inguinal lymph node cells show a decrease in CD4+ Il-4 producing cells compared to wild-type

• qIfng/Il-4 ratio is significantly increased compared to wild-type

decreased susceptibility to parasitic infection ( J:113851 )

• by 9 weeks, mice develop markedly smaller lesions (5 mm) compared to wild-type (9 mm) upon subcutaneous inoculation with L. mexicana

• parasitic burden is reduced 100-fold compared to wild-type

• inflammation is substantially reduced with fewer parasitized macrophages and absence of tissue damage compared to wild-type

Genotype
MGI:4821796

cx2

• Allelic Composition: Cd274^tm2Shr/Cd274^tm2Shr; Pdcd1lg2^tm4Shr/Pdcd1lg2^tm4Shr
• Genetic Background: involves: 129S4/SvJae

immune system

abnormal immune system physiology ( J:160970 )

• mice are reported to exhibit a defect in T celll tolerance and increased immune activation