Phenotypes associated with this allele

• Allele Symbol: Lmnb1^Gt(XA130)Byg
• Allele Name: gene trap XA130, BayGenomics
• Allele ID: MGI:3050819
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lmnb1^Gt(XA130)Byg/Lmnb1^Gt(XA130)Byg	involves: 129P2/OlaHsd * C57BL/6J	MGI:3051123

Genotype
MGI:3051123

hm1

• Allelic Composition: Lmnb1^Gt(XA130)Byg/Lmnb1^Gt(XA130)Byg
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Appearance of Lmnb1^Gt(XA130)Byg/Lmnb1^Gt(XA130)Byg embryos

mortality/aging

neonatal lethality, complete penetrance ( J:91473 )

• homozygous pups die within a few minutes after birth from respiratory failure

cellular

abnormal cell nucleus morphology ( J:91473 )

• multiple large blebs are seen in 38-39% of homozygous null primary MEF nuclei

abnormal cell differentiation ( J:91473 )

• homozygous null primary MEFs fail to differentiate under culture conditions in which wild-type and heterozygous primary MEFs differentiate

decreased cell proliferation ( J:91473 )

• homozygous null primary MEFs display reduced proliferative potential in culture, reaching proliferative crisis sooner compared to wild-type and heterozygous primary MEFs

• more than 80% of homozygous null primary MEFs were polyploid after 4 passag

craniofacial

abnormal cranium morphology ( J:91473 )

• the skull appears flatter than normal

abnormal coronal suture morphology ( J:91473 )

• the coronal suture is narrowed and the sagital suture obscured by bone overlap

abnormal sagittal suture morphology ( J:91473 )

• the sagital suture obscured by bone overlap

embryo

decreased embryo size ( J:91473 )

• embryos are smaller than normal

growth/size/body

decreased embryo size ( J:91473 )

• embryos are smaller than normal

respiratory system

abnormal lung morphology ( J:91473 )

• the lungs are abnormal in 9 out of 11 embryos at E18.5

• the mesenchymal tissue is thicker compared to wild-type mice

absent pulmonary alveoli ( J:91473 )

• in 2 of the embryos (out of 11) no alveoli are seen

respiratory failure ( J:91473 )

• homozygous pups die within a few minutes after birth from respiratory failure

skeleton

abnormal cranium morphology ( J:91473 )

• the skull appears flatter than normal

abnormal coronal suture morphology ( J:91473 )

• the coronal suture is narrowed and the sagital suture obscured by bone overlap

abnormal sagittal suture morphology ( J:91473 )

• the sagital suture obscured by bone overlap

abnormal thoracic vertebrae morphology ( J:91473 )

• distortion of the thoracic vertebral column is seen

abnormal spine curvature ( J:91473 )

• embryos display an abnormal curvature of the spine

delayed bone ossification ( J:91473 )

• at E18.5 reduced Alizarin red staining of many bones suggests delayed or impaired ossification