All Phenotypes Tg(TcraTcrb)8Rest MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pgd^tm1.1Pse/Pgd^tm1.1Pse Tg(Cd4-cre)1Cwi/0 Tg(TcraTcrb)8Rest/0	involves: C57BL/6 * DBA/2	MGI:6751656
cx2	Ctla4^tm1All/Ctla4^tm1All Rag1^tm1Mom/Rag1^tm1Mom Tg(TcraTcrb)8Rest/0	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6	MGI:4940117
cx3	Ctla4^tm1All/Ctla4^tm1All Tg(TcraTcrb)8Rest/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:4940116
tg4	Tg(TcraTcrb)8Rest/?	C57BL/6-Tg(TcraTcrb)8Rest	MGI:3789128

Allelic Composition	Pgd^tm1.1Pse/Pgd^tm1.1Pse Tg(Cd4-cre)1Cwi/0 Tg(TcraTcrb)8Rest/0
Genetic Background	involves: C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pgd^tm1.1Pse mutation (0 available); any Pgd mutation (21 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
Tg(TcraTcrb)8Rest mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell physiology ( J:306553 )

• CD8+ T cells co-cultured with B16-F10 melanoma cells uptake glucose more effectively than control cells

• adaptive transfer of mutant CD8+ T cells to congenic recipients bearing the B16-F10 melanoma results in smaller tumor growth than in controls

hematopoietic system

abnormal CD8-positive, alpha-beta T cell physiology ( J:306553 )

• CD8+ T cells co-cultured with B16-F10 melanoma cells uptake glucose more effectively than control cells

• adaptive transfer of mutant CD8+ T cells to congenic recipients bearing the B16-F10 melanoma results in smaller tumor growth than in controls

Allelic Composition	Ctla4^tm1All/Ctla4^tm1All Rag1^tm1Mom/Rag1^tm1Mom Tg(TcraTcrb)8Rest/0
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctla4^tm1All mutation (2 available); any Ctla4 mutation (54 available)
Rag1^tm1Mom mutation (49 available); any Rag1 mutation (123 available)
Tg(TcraTcrb)8Rest mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:140448 )

N	• mice exhibit a normal lifespan

immune system

immune system phenotype ( J:140448 )

N	• CD8+ T cells exhibit a completely naive phenotype

pigmentation

pigmentation phenotype ( J:140448 )

N	• mice do not develop autoimmune vitiligo

Allelic Composition	Ctla4^tm1All/Ctla4^tm1All Tg(TcraTcrb)8Rest/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctla4^tm1All mutation (2 available); any Ctla4 mutation (54 available)
Tg(TcraTcrb)8Rest mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:140448 )

• mice die at 10 to 12 weeks

immune system

increased CD4-positive, alpha-beta T cell number ( J:140448 )

decreased CD8-positive, alpha-beta T cell number ( J:140448 )

increased interferon-gamma secretion ( J:140448 )

• hgp100(25-33)-treated splenocyte exhibit increased IFN-gamma secretion compared with splenocytes from Tg(TcraTcrb)8Rest mice

pigmentation

variable depigmentation ( J:140448 )

• at 4 to 6 weeks, 48 of 49 mice develop autoimmune vitiligo unlike Tg(TcraTcrb)8Rest mice

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:140448 )

decreased CD8-positive, alpha-beta T cell number ( J:140448 )

integument

variable depigmentation ( J:140448 )

• at 4 to 6 weeks, 48 of 49 mice develop autoimmune vitiligo unlike Tg(TcraTcrb)8Rest mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:85058 )

• over 95 % of the CD8 T cells present in circulation express the transgenic TCR that is specific for the H2-D^b restricted epitope of Silv (gp100), a protein that is highly expressed in melanoma cells

• these transgenic T cells constitute about 20% of spleen cells

• the baseline levels of activation markers for the transgenic T cells indicate that most of cells are in a nave state

abnormal cytotoxic T cell physiology ( J:85058 )

• upon in vitro stimulation with the immunogenic epitope of gp100, CD8 T cells are activated and proliferate extensively

increased interferon-gamma secretion ( J:85058 )

• CD 8 T cells produce large amounts of IFN-gamma when cultured in the presence of melanoma cells

• large number of IFN-gamma producing CD8 T cells are found in melanoma tumors that have regressed due to treatment with gp100 vaccine, IL-2, and adoptive transfer of transgenic CD8 T cells

neoplasm

abnormal tumor susceptibility ( J:85058 )

• despite the presence of large number of anti-gp100 CD8 T cells, transplanted melanoma tumors that express gp100 have the same growth kinetics as tumors transplanted into control mice

• adoptive transfer of transgenic splenocytes into normal mice bearing melanoma tumors also has no effect on tumor growth

• there is a modest delay in tumor growth when mice that are recipients of transgenic T cells are also vaccinated with peptides that mimic the gp100 epitope

• when IL-2 is administered with the vaccine, there is a large regression in tumor size in mice that are also recipients of transgenic T cells

• mice bearing melanoma tumors that receive vaccine, transgenic T cells, and IL-2 have survival times of over 1 year compared to controls that die within 2 months

hematopoietic system

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:85058 )

• these transgenic T cells constitute about 20% of spleen cells

• the baseline levels of activation markers for the transgenic T cells indicate that most of cells are in a nave state

abnormal cytotoxic T cell physiology ( J:85058 )

• upon in vitro stimulation with the immunogenic epitope of gp100, CD8 T cells are activated and proliferate extensively

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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