Phenotypes associated with this allele

• Allele Symbol: Tg(TcrAND)53Hed
• Allele Name: transgene insertion 53, Stephen M Hedrick
• Allele ID: MGI:3053044
• Summary: 31 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda Rag2^tm1.1Cgn/Rag2^tm1.1Cgn Tg(Lck-cre)548Jxm/0 Tg(TcrAND)53Hed/0	B6.Cg-Rag2^tm1.1Cgn Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm Tg(TcrAND)53Hed	MGI:5447537
cn2	Tcf3^tm4Zhu/Tcf3^tm4Zhu Tg(Lck-cre)#Zhu/0 Tg(TcrAND)53Hed/0	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:3774552
cn3	Notch1^tm1Agt/Notch1^tm1Agt Notch2^tm3Grid/Notch2^tm3Grid Tg(TcrAND)53Hed/?	involves: 129/Sv * 129S1/Sv * C57BL/6 * SJL	MGI:3720331
cn4	Mapk1^tm1Hed/Mapk1^tm1Hed Mapk3^tm1Gpg/Mapk3^tm1Gpg Tg(Cd4-cre)1Cwi/0 Tg(TcrAND)53Hed/0	involves: 129/Sv * C57BL/6 * DBA/2 * SJL	MGI:3687238
cn5	Mapk1^tm1Hed/Mapk1^tm1Hed Tg(Lck-cre)1Cwi/0 Tg(TcrAND)53Hed/0	involves: C57BL/6 * DBA/2 * SJL	MGI:3687239
cx6	Cd4^tm1Jrp/Cd4^tm1Jrp Tg(TcrAND)53Hed/0	either: (involves: 129S2/SvPas * C57BL/6 * SJL) or (involves: 129S2/SvPas * C57BL/6 * C57BL/10 * SJL)	MGI:3652735
cx7	Tg(TcrAND)53Hed/? Themis^tm1Lov/Themis^tm1Lov	involves: 129 * C57BL/6 * SJL	MGI:4353389
cx8	Ctla4^tm1All/Ctla4^tm1All Tg(TcrAND)53Hed/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:4940114
cx9	Rag1^tm1Bal/Rag1^tm1Bal Vav1^tm2Tyb/Vav1^tm2Tyb Tg(TcrAND)53Hed/0	involves: 129S4/SvJae * 129S8/SvEv * C57BL/6 * SJL	MGI:3689309
cx10	Itk^tm1Litt/Itk^tm1Litt Tg(TcrAND)53Hed/0	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:3771701
cx11	Lcp2^tm3Gak/Lcp2^tm3Gak Tg(TcrAND)53Hed/?	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:3778798
cx12	Lcp2^tm4Gak/Lcp2^tm4Gak Tg(TcrAND)53Hed/?	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:3778802
cx13	Tg(TcrAND)53Hed/? Themis^tm1Gasc/Themis^tm1Gasc	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:4353115
cx14	Ctla4^tm1All/Ctla4^tm1All Rag1^tm1Mom/Rag1^tm1Mom Tg(TcrAND)53Hed/0	involves: 129S/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:4940115
cx15	Rac2^tm1Mddw/Rac2^tm1Mddw Tg(TcrAND)53Hed/0	involves: 129S/SvEv * C57BL/6 * SJL	MGI:3840504
cx16	Dock8^tm1Ysfk/Dock8^tm1Ysfk Osmr^tm1Mtan/Osmr^tm1Mtan Tg(TcrAND)53Hed/0	involves: 129S/SvEv * C57BL/6 * SJL	MGI:7643972
cx17	Il15ra^tm1.1Nsl/Il15ra^tm1.1Nsl Tg(TcrAND)53Hed/?	involves: 129X1/SvJ * C57BL/6 * SJL	MGI:3845657
cx18	H2^b/H2^k Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354598
cx19	H2^b/H2^b Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354597
cx20	Mapk1^tm1Hed/Mapk1^tm1Hed Tg(TcrAND)53Hed/0	involves: C57BL/6 * DBA/2 * SJL	MGI:3687237
cx21	Dock8^tm1Ysfk/Dock8^tm1Ysfk Tac2^em1Ysfk/Tac2^em1Ysfk Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:7643975
cx22	Tg(TcrAND)53Hed/? Zbtb7b^tm1.1Rbo/Zbtb7b^tm1.1Rbo	involves: C57BL/6 * SJL	MGI:3817639
cx23	Tg(H2KmPCC)2939Stoe/? Tg(TcrAND)53Hed/?	involves: C57BL/6 * SJL	MGI:3835458
cx24	Tcf12^tm2Zhu/Tcf12^tm2Zhu Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:3709891
cx25	Cd3e^tm1.1Geno/Cd3e^tm1.1Geno Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:5474204
cx26	Rr381^em2Kap/Rr381^em2Kap Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:7439199
cx27	Dock8^tm1Ysfk/Dock8^tm1Ysfk Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:7643971
cx28	Dock8^tm1Ysfk/Dock8^tm1Ysfk Tac2^em2Ysfk/Tac2^em2Ysfk Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:7643976
cx29	Dock8^tm1Ysfk/Dock8^tm1Ysfk Il31^tm1.1Ysfk/Il31^tm1.1Ysfk Tg(TcrAND)53Hed/0	involves: C57BL/6 * SJL	MGI:7643974
tg30	Tg(TcrAND)53Hed/?	involves: A/WySnSg * C57BL/6 * C57BL/10SnSg * SJL	MGI:3784173
tg31	Tg(TcrAND)53Hed/?	involves: C57BL/6 * SJL	MGI:3835451

Genotype
MGI:5447537

cn1

• Allelic Composition: Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda; Rag2^tm1.1Cgn/Rag2^tm1.1Cgn; Tg(Lck-cre)548Jxm/0; Tg(TcrAND)53Hed/0
• Genetic Background: B6.Cg-Rag2^tm1.1Cgn Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm Tg(TcrAND)53Hed

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased double-positive T cell number ( J:189951 )

• in the thymus

hematopoietic system

decreased double-positive T cell number ( J:189951 )

• in the thymus

Genotype
MGI:3774552

cn2

• Allelic Composition: Tcf3^tm4Zhu/Tcf3^tm4Zhu; Tg(Lck-cre)#Zhu/0; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

immune system

immune system phenotype ( J:75746 )

N • TCR mediated positive T cell selection is unaffected by Tcfe2a deletion in T cells, in contrast to mice with total Tcfe2a deficiency

neoplasm

neoplasm ( J:75746 )

N • in contrast to totally Tcfe2a-deficient mice, T cell specific Tcfe2a-deficient mice do not develop thymic lymphomas between 4 and 16 months of age

Genotype
MGI:3720331

cn3

• Allelic Composition: Notch1^tm1Agt/Notch1^tm1Agt; Notch2^tm3Grid/Notch2^tm3Grid; Tg(TcrAND)53Hed/?
• Genetic Background: involves: 129/Sv * 129S1/Sv * C57BL/6 * SJL

immune system

abnormal humoral immune response ( J:123631 )

• the immune response to strong T helper2 inducing conditions is absent

decreased interleukin-4 secretion ( J:123631 )

• after 5 days of culture of antigen presenting cells with Schistosoma mansoni soluble egg antigen and pigeon cytochrome C, no IL-4 is produced as it is in wild-type cells

abnormal response to infection ( J:123631 )

• after 5 days of culture of antigen presenting cells with Schistosoma mansoni soluble egg antigen and pigeon cytochrome C, no IL-4 is produced as it is in wild-type cells

Genotype
MGI:3687238

cn4

• Allelic Composition: Mapk1^tm1Hed/Mapk1^tm1Hed; Mapk3^tm1Gpg/Mapk3^tm1Gpg; Tg(Cd4-cre)1Cwi/0; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129/Sv * C57BL/6 * DBA/2 * SJL

immune system

abnormal positive T cell selection ( J:113280 )

• positive selection is diminished significantly

abnormal T cell subpopulation ratio ( J:113280 )

• CD4 to CD8 ratio (% of mature CD4 and CD8 T cells) is 24 compared to 257 in non transgenic littermates

hematopoietic system

abnormal positive T cell selection ( J:113280 )

• positive selection is diminished significantly

abnormal T cell subpopulation ratio ( J:113280 )

• CD4 to CD8 ratio (% of mature CD4 and CD8 T cells) is 24 compared to 257 in non transgenic littermates

Genotype
MGI:3687239

cn5

• Allelic Composition: Mapk1^tm1Hed/Mapk1^tm1Hed; Tg(Lck-cre)1Cwi/0; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

abnormal positive T cell selection ( J:113280 )

• positive selection is diminished significantly

abnormal T cell subpopulation ratio ( J:113280 )

• CD4 to CD8 ratio (% of mature CD4 and CD8 T cells) is 20 compared to 257 in non transgenic littermates

hematopoietic system

abnormal positive T cell selection ( J:113280 )

• positive selection is diminished significantly

abnormal T cell subpopulation ratio ( J:113280 )

• CD4 to CD8 ratio (% of mature CD4 and CD8 T cells) is 20 compared to 257 in non transgenic littermates

Genotype
MGI:3652735

cx6

• Allelic Composition: Cd4^tm1Jrp/Cd4^tm1Jrp; Tg(TcrAND)53Hed/0
• Genetic Background: either: (involves: 129S2/SvPas * C57BL/6 * SJL) or (involves: 129S2/SvPas * C57BL/6 * C57BL/10 * SJL)

immune system

thymus hyperplasia ( J:45168 )

• thymi have considerably greater cellularity (33-113% more cells) than littermates

abnormal T cell number ( J:45168 )

• mutant thymus has a 58% reduction in CD4+ thymocytes compared to littermates that only expressing the transgene

hematopoietic system

thymus hyperplasia ( J:45168 )

• thymi have considerably greater cellularity (33-113% more cells) than littermates

abnormal T cell number ( J:45168 )

• mutant thymus has a 58% reduction in CD4+ thymocytes compared to littermates that only expressing the transgene

endocrine/exocrine glands

thymus hyperplasia ( J:45168 )

• thymi have considerably greater cellularity (33-113% more cells) than littermates

Genotype
MGI:4353389

cx7

• Allelic Composition: Tg(TcrAND)53Hed/?; Themis^tm1Lov/Themis^tm1Lov
• Genetic Background: involves: 129 * C57BL/6 * SJL

immune system

abnormal positive T cell selection ( J:151075 )

• higher double positive thymocyte numbers and fewer single-positive thymoyctes indicate a defect in positive selection

increased double-positive T cell number ( J:151075 )

• double-positive thymocytes are increased in mice

decreased single-positive T cell number ( J:151075 )

• CD4 single-positive thymocyte numbers are reduced in these mice

hematopoietic system

abnormal positive T cell selection ( J:151075 )

• higher double positive thymocyte numbers and fewer single-positive thymoyctes indicate a defect in positive selection

increased double-positive T cell number ( J:151075 )

• double-positive thymocytes are increased in mice

decreased single-positive T cell number ( J:151075 )

• CD4 single-positive thymocyte numbers are reduced in these mice

Genotype
MGI:4940114

cx8

• Allelic Composition: Ctla4^tm1All/Ctla4^tm1All; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

mortality/aging

premature death ( J:119845 )

• mice die at 8 to 12 weeks of age

immune system

abnormal lymphopoiesis ( J:119845 )

• onset of lymphoproliferation is delayed compared to in Ctla4^tm1All homozygotes

abnormal T cell subpopulation ratio ( J:119845 )

• mice exhibit the expected skewing toward CD4+ T cells as in Tg(TcrAND)53Hed compared with wild-type mice

increased CD4-positive, alpha-beta T cell number ( J:119845 )

• greater than 10-fold

increased CD8-positive, alpha-beta T cell number ( J:119845 )

• in absolute numbers compared with Tg(TcrAND)53Hed mice

abnormal T cell activation ( J:119845 )

• at 4 weeks, nearly all CD4+ T cells exhibit an activated phenotype unlike in Tg(TcrAND)53Hed mice

hematopoietic system

abnormal lymphopoiesis ( J:119845 )

• onset of lymphoproliferation is delayed compared to in Ctla4^tm1All homozygotes

abnormal T cell subpopulation ratio ( J:119845 )

• mice exhibit the expected skewing toward CD4+ T cells as in Tg(TcrAND)53Hed compared with wild-type mice

increased CD4-positive, alpha-beta T cell number ( J:119845 )

• greater than 10-fold

increased CD8-positive, alpha-beta T cell number ( J:119845 )

• in absolute numbers compared with Tg(TcrAND)53Hed mice

abnormal T cell activation ( J:119845 )

• at 4 weeks, nearly all CD4+ T cells exhibit an activated phenotype unlike in Tg(TcrAND)53Hed mice

Genotype
MGI:3689309

cx9

• Allelic Composition: Rag1^tm1Bal/Rag1^tm1Bal; Vav1^tm2Tyb/Vav1^tm2Tyb; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S4/SvJae * 129S8/SvEv * C57BL/6 * SJL

immune system

decreased CD4-positive, alpha-beta T cell number ( J:113270 )

• reduced percentage of CD4+ cells compared to mice wild-type for Vav1

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:113270 )

• reduced percentage of CD4+ cells compared to mice wild-type for Vav1

Genotype
MGI:3771701

cx10

• Allelic Composition: Itk^tm1Litt/Itk^tm1Litt; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

immune system

abnormal lymphocyte morphology ( J:31230 )

• CD4+ T cells that do develop display sginficant reduction in expression of TCR alpha chain (40-50% compared to 90% in heterozygous mice littermates)

increased double-negative T cell number ( J:31230 )

• number of CD4-CD8- T cells is increased 5-7-fold compared to Itk-null mice

decreased CD4-positive, alpha-beta T cell number ( J:31230 )

• dramatic reduction in number and proportion of CD4+CD8- thymocytes is observed in thymus relative to control Itk-null mices

• profound reduction in CD4+ T cells in periphery is seen

hematopoietic system

abnormal lymphocyte morphology ( J:31230 )

• CD4+ T cells that do develop display sginficant reduction in expression of TCR alpha chain (40-50% compared to 90% in heterozygous mice littermates)

increased double-negative T cell number ( J:31230 )

• number of CD4-CD8- T cells is increased 5-7-fold compared to Itk-null mice

decreased CD4-positive, alpha-beta T cell number ( J:31230 )

• dramatic reduction in number and proportion of CD4+CD8- thymocytes is observed in thymus relative to control Itk-null mices

• profound reduction in CD4+ T cells in periphery is seen

Genotype
MGI:3778798

cx11

• Allelic Composition: Lcp2^tm3Gak/Lcp2^tm3Gak; Tg(TcrAND)53Hed/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

immune system

abnormal thymocyte activation ( J:132995 )

• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes

• upon conjugation, thymocytes have impaired TCR-induced actin polymerization

abnormal positive T cell selection ( J:132995 )

• there is an approximate 75% loss of transgenic TCR expression by CD4 single positive cells in the thymus

• the percentage of CD4 single positive T cells in the thymus is reduced almost 20-fold compared mice carrying the transgenic TCR alone

hematopoietic system

abnormal thymocyte activation ( J:132995 )

• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes

• upon conjugation, thymocytes have impaired TCR-induced actin polymerization

abnormal positive T cell selection ( J:132995 )

• there is an approximate 75% loss of transgenic TCR expression by CD4 single positive cells in the thymus

• the percentage of CD4 single positive T cells in the thymus is reduced almost 20-fold compared mice carrying the transgenic TCR alone

endocrine/exocrine glands

abnormal thymocyte activation ( J:132995 )

• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes

• upon conjugation, thymocytes have impaired TCR-induced actin polymerization

Genotype
MGI:3778802

cx12

• Allelic Composition: Lcp2^tm4Gak/Lcp2^tm4Gak; Tg(TcrAND)53Hed/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

immune system

abnormal thymocyte activation ( J:132995 )

• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes

• upon conjugation, thymocytes have impaired TCR-induced actin polymerization

abnormal positive T cell selection ( J:132995 )

• the percentage of CD4 single positive T cells in the thymus is reduced almost 10-fold compared mice carrying the transgenic TCR alone

hematopoietic system

abnormal thymocyte activation ( J:132995 )

• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes

• upon conjugation, thymocytes have impaired TCR-induced actin polymerization

abnormal positive T cell selection ( J:132995 )

• the percentage of CD4 single positive T cells in the thymus is reduced almost 10-fold compared mice carrying the transgenic TCR alone

endocrine/exocrine glands

abnormal thymocyte activation ( J:132995 )

• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes

• upon conjugation, thymocytes have impaired TCR-induced actin polymerization

Genotype
MGI:4353115

cx13

• Allelic Composition: Tg(TcrAND)53Hed/?; Themis^tm1Gasc/Themis^tm1Gasc
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

immune system

abnormal positive T cell selection ( J:151074 )

• positive selection is almost completely blocked in these mice

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is greater than controls

decreased CD4-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

• peripheral CD4 T cell numbers are severely reduced

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

hematopoietic system

abnormal positive T cell selection ( J:151074 )

• positive selection is almost completely blocked in these mice

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is greater than controls

decreased CD4-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

• peripheral CD4 T cell numbers are severely reduced

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

Genotype
MGI:4940115

cx14

• Allelic Composition: Ctla4^tm1All/Ctla4^tm1All; Rag1^tm1Mom/Rag1^tm1Mom; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S/Sv * 129X1/SvJ * C57BL/6 * SJL

immune system

abnormal T cell activation ( J:119845 )

• nearly all CD4+ T cells in aged mice exhibit an activated phenotype unlike in Tg(TcrAND)53Hed mice

• however, lymph node T cells maintain a naive phenotype in young mice

increased T cell proliferation ( J:119845 )

• CD4+ T cells exhibit increased primary and secondary antigen-specific proliferative responses compared to in Rag1^tm1Mom/Rag1^tm1Mom Tg(TcrAND)53Hed mice

increased interleukin-4 secretion ( J:119845 )

• in response to primary and secondary pigeon cytochrome c stimulation, the frequency of IL4-secreting T cells is increased compared to in Rag1^tm1Mom/Rag1^tm1Mom Tg(TcrAND)53Hed mice

hematopoietic system

abnormal T cell activation ( J:119845 )

• nearly all CD4+ T cells in aged mice exhibit an activated phenotype unlike in Tg(TcrAND)53Hed mice

• however, lymph node T cells maintain a naive phenotype in young mice

increased T cell proliferation ( J:119845 )

• CD4+ T cells exhibit increased primary and secondary antigen-specific proliferative responses compared to in Rag1^tm1Mom/Rag1^tm1Mom Tg(TcrAND)53Hed mice

cellular

increased T cell proliferation ( J:119845 )

• CD4+ T cells exhibit increased primary and secondary antigen-specific proliferative responses compared to in Rag1^tm1Mom/Rag1^tm1Mom Tg(TcrAND)53Hed mice

Genotype
MGI:3840504

cx15

• Allelic Composition: Rac2^tm1Mddw/Rac2^tm1Mddw; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * SJL

immune system

abnormal T cell activation ( J:119437 )

• following TCR stimulation, calcium mobilization in CD4+ T cells is decreased compared to in similarly treated wild-type cells

decreased T cell proliferation ( J:119437 )

• T cell proliferation is 2- to 3-fold lower following stimulation with I-E^K+ or fibroblast APC expressing with I-E^K+ and B7 molecules compared to in similarly treated wild-type cells

decreased circulating interleukin-2 level ( J:119437 )

• following stimulation with TCR-specific antigen

homeostasis/metabolism

decreased circulating interleukin-2 level ( J:119437 )

• following stimulation with TCR-specific antigen

hematopoietic system

abnormal T cell activation ( J:119437 )

• following TCR stimulation, calcium mobilization in CD4+ T cells is decreased compared to in similarly treated wild-type cells

decreased T cell proliferation ( J:119437 )

• T cell proliferation is 2- to 3-fold lower following stimulation with I-E^K+ or fibroblast APC expressing with I-E^K+ and B7 molecules compared to in similarly treated wild-type cells

cellular

decreased T cell proliferation ( J:119437 )

• T cell proliferation is 2- to 3-fold lower following stimulation with I-E^K+ or fibroblast APC expressing with I-E^K+ and B7 molecules compared to in similarly treated wild-type cells

Genotype
MGI:7643972

cx16

• Allelic Composition: Dock8^tm1Ysfk/Dock8^tm1Ysfk; Osmr^tm1Mtan/Osmr^tm1Mtan; Tg(TcrAND)53Hed/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * SJL

integument

integument phenotype ( J:305132 )

N • mice show improved skin inflammation and scratching behavior and do not develop atopic dermatitis-like skin disease that is seen in Dock8^tm1Ysfk/Dock8^tm1Ysfk Tg(TcrAND)53Hed/0 mice

Genotype
MGI:3845657

cx17

• Allelic Composition: Il15ra^tm1.1Nsl/Il15ra^tm1.1Nsl; Tg(TcrAND)53Hed/?
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * SJL

immune system

increased T cell proliferation ( J:92705 )

• the activation threshold for transgenic CD4⁺ T cells is much lower than wild-type transgenic T cells

• both weak and strong peptide agonists caused increased amounts of proliferation compared to controls

hematopoietic system

increased T cell proliferation ( J:92705 )

• the activation threshold for transgenic CD4⁺ T cells is much lower than wild-type transgenic T cells

• both weak and strong peptide agonists caused increased amounts of proliferation compared to controls

cellular

increased T cell proliferation ( J:92705 )

• the activation threshold for transgenic CD4⁺ T cells is much lower than wild-type transgenic T cells

• both weak and strong peptide agonists caused increased amounts of proliferation compared to controls

Genotype
MGI:4354598

cx18

• Allelic Composition: H2^b/H2^k; Itk^tm1Ljb/Itk^tm1Ljb; Tg(TcrAND)53Hed/?
• Genetic Background: involves: C3H/HeJ * C57BL/6 * C57BL/10

immune system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the development of transgenic CD4 T cells is reduced 2- to 3-fold in these mice

hematopoietic system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the development of transgenic CD4 T cells is reduced 2- to 3-fold in these mice

Genotype
MGI:4354597

cx19

• Allelic Composition: H2^b/H2^b; Itk^tm1Ljb/Itk^tm1Ljb; Tg(TcrAND)53Hed/?
• Genetic Background: involves: C3H/HeJ * C57BL/6 * C57BL/10

immune system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• there are almost no mature transgenic CD4 T cells in the periphery

hematopoietic system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• there are almost no mature transgenic CD4 T cells in the periphery

Genotype
MGI:3687237

cx20

• Allelic Composition: Mapk1^tm1Hed/Mapk1^tm1Hed; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

abnormal positive T cell selection ( J:113280 )

• reduction in TCRhi, CD69hi and CD69lo indicate inefficient positive selection

abnormal double-positive T cell morphology ( J:113280 )

• mice show altered proportion of double positive and CD4 populations

hematopoietic system

abnormal positive T cell selection ( J:113280 )

• reduction in TCRhi, CD69hi and CD69lo indicate inefficient positive selection

abnormal double-positive T cell morphology ( J:113280 )

• mice show altered proportion of double positive and CD4 populations

Genotype
MGI:7643975

cx21

• Allelic Composition: Dock8^tm1Ysfk/Dock8^tm1Ysfk; Tac2^em1Ysfk/Tac2^em1Ysfk; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

integument

integument phenotype ( J:305132 )

N • mice show improved skin inflammation and scratching behavior compared to Dock8^tm1Ysfk/Dock8^tm1Ysfk Tg(TcrAND)53Hed/0 mice, without affecting serum IL-31 levels

Genotype
MGI:3817639

cx22

• Allelic Composition: Tg(TcrAND)53Hed/?; Zbtb7b^tm1.1Rbo/Zbtb7b^tm1.1Rbo
• Genetic Background: involves: C57BL/6 * SJL

immune system

abnormal T cell differentiation ( J:141145 )

• T cells expressing the transgenic MHC class II-restricted TCR show diversion from the CD4⁺ lineage to the CD8⁺ lineage

hematopoietic system

abnormal T cell differentiation ( J:141145 )

• T cells expressing the transgenic MHC class II-restricted TCR show diversion from the CD4⁺ lineage to the CD8⁺ lineage

Genotype
MGI:3835458

cx23

• Allelic Composition: Tg(H2KmPCC)2939Stoe/?; Tg(TcrAND)53Hed/?
• Genetic Background: involves: C57BL/6 * SJL

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:92997 )

• the number of CD4 single-positive thymocytes are severely reduced in these mice

• peripheral CD4⁺ T cells are reduced by 75%

immune system

decreased CD4-positive, alpha-beta T cell number ( J:92997 )

• the number of CD4 single-positive thymocytes are severely reduced in these mice

• peripheral CD4⁺ T cells are reduced by 75%

abnormal immune tolerance ( J:92997 )

• negative selection of transgenic T cells leads to diminished CD4 T cells in the periphery

• apoptotic cells resulting from negative selection are found at the cortico-medullary and medullary portions of the thymus

abnormal T cell anergy ( J:92997 )

• T cells that escape central tolerance are able to respond to pigeon cytochrome C but 10 times as much protein is needed to get a comparable response to Tg(TcrAND)53Hed transgenic

Genotype
MGI:3709891

cx24

• Allelic Composition: Tcf12^tm2Zhu/Tcf12^tm2Zhu; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

immune system

arrested T cell differentiation ( J:120411 )

• block in T cell development is not rescued by transgene expression

abnormal T cell subpopulation ratio ( J:120411 )

• increased ratio of CD4 SP to DP cells is detected in thymus

increased T cell number ( J:120411 )

• no increase in the double positive T cell population or thymic cellularity relative to Tcf12^tm2Zhu homozygotes

• an increase in CD4 single positive cells is seen, indicating transgene expression can drive differentiation from the double positive (DP) to single positive (SP) stage

abnormal CD4-positive, alpha beta T cell morphology ( J:120411 )

• number of CD4 SP T cells is increased relative to nontransgenic Tcf12-null mice

• numbers of CD4 SP cells in thymus and peripheral lymphoid organs remains small relative to controls

hematopoietic system

arrested T cell differentiation ( J:120411 )

• block in T cell development is not rescued by transgene expression

abnormal T cell subpopulation ratio ( J:120411 )

• increased ratio of CD4 SP to DP cells is detected in thymus

increased T cell number ( J:120411 )

• no increase in the double positive T cell population or thymic cellularity relative to Tcf12^tm2Zhu homozygotes

• an increase in CD4 single positive cells is seen, indicating transgene expression can drive differentiation from the double positive (DP) to single positive (SP) stage

abnormal CD4-positive, alpha beta T cell morphology ( J:120411 )

• number of CD4 SP T cells is increased relative to nontransgenic Tcf12-null mice

• numbers of CD4 SP cells in thymus and peripheral lymphoid organs remains small relative to controls

Genotype
MGI:5474204

cx25

• Allelic Composition: Cd3e^tm1.1Geno/Cd3e^tm1.1Geno; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

immune system

increased DN3 thymocyte number ( J:192604 )

abnormal CD4-positive T cell differentiation ( J:192604 )

• decrease in the formation of CD4 single positive thymocytes

abnormal positive T cell selection ( J:192604 )

hematopoietic system

increased DN3 thymocyte number ( J:192604 )

abnormal CD4-positive T cell differentiation ( J:192604 )

• decrease in the formation of CD4 single positive thymocytes

abnormal positive T cell selection ( J:192604 )

endocrine/exocrine glands

increased DN3 thymocyte number ( J:192604 )

Genotype
MGI:7439199

cx26

• Allelic Composition: Rr381^em2Kap/Rr381^em2Kap; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

hematopoietic system

hematopoietic system phenotype ( J:333471 )

N • normal MHC class-specific T cell development

Genotype
MGI:7643971

cx27

• Allelic Composition: Dock8^tm1Ysfk/Dock8^tm1Ysfk; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

immune system

dermatitis ( J:305132 )

• mice develop an atopic dermatitis-like skin disease, with increased skin inflammation and scratching behavior

integument

dermatitis ( J:305132 )

• mice develop an atopic dermatitis-like skin disease, with increased skin inflammation and scratching behavior

increased pruritus ( J:305132 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
atopic dermatitis		DOID:3310	OMIM:603165 OMIM:PS603165	J:305132

Genotype
MGI:7643976

cx28

• Allelic Composition: Dock8^tm1Ysfk/Dock8^tm1Ysfk; Tac2^em2Ysfk/Tac2^em2Ysfk; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

integument

integument phenotype ( J:305132 )

N • mice show improved skin inflammation and scratching behavior compared to Dock8^tm1Ysfk/Dock8^tm1Ysfk Tg(TcrAND)53Hed/0 mice, without affecting serum IL-31 levels

Genotype
MGI:7643974

cx29

• Allelic Composition: Dock8^tm1Ysfk/Dock8^tm1Ysfk; Il31^tm1.1Ysfk/Il31^tm1.1Ysfk; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * SJL

integument

integument phenotype ( J:305132 )

N • mice show improved skin inflammation and scratching behavior and do not develop atopic dermatitis-like skin disease that is seen in Dock8^tm1Ysfk/Dock8^tm1Ysfk Tg(TcrAND)53Hed/0 mice

Genotype
MGI:3784173

tg30

• Allelic Composition: Tg(TcrAND)53Hed/?
• Genetic Background: involves: A/WySnSg * C57BL/6 * C57BL/10SnSg * SJL

immune system

abnormal T cell proliferation ( J:85777 )

• T cells proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells

abnormal thymocyte activation ( J:85777 )

• thymocytes proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells

abnormal T cell differentiation ( J:85777 )

• 75% of thymocytes express high levels of CD3 compared to 18% of wild-type thymocytes

• 99% of thymocytes have detectable levels of CD3 compared to only 75% in wild-type thymocytes

• virtually all CD4 T cells in the periphery express the transgenic TCR

abnormal positive T cell selection ( J:85777 )

• the transgenic TCR is positively selected for on this genetic background (H2-E^k) leading to a more efficient positive T cell selection that is skewed towards CD4 T cell production

decreased double-positive T cell number ( J:85777 )

• the percentage of double-positive T cells in the thymus is 24% compared to 83% in wild-type mice

abnormal T cell subpopulation ratio ( J:85777 )

• the CD4/CD8 single positive T cell ratio in the thymus is heavily skewed towards the CD4 population

increased CD4-positive, alpha-beta T cell number ( J:85777 )

• the percentage of CD4 single positive T cells in the thymus is 63% compared to 13% in wild-type mice

• the percentage of CD 4 T cells in lymph nodes is 74% compared to 44% in wild-type controls

decreased CD8-positive, alpha-beta T cell number ( J:85777 )

• there is a two-fold reduction in CD8 single positive T cells in the thymus

• CD8 T cells are severely reduced in peripheral lymph nodes

hematopoietic system

abnormal T cell proliferation ( J:85777 )

• T cells proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells

abnormal thymocyte activation ( J:85777 )

• thymocytes proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells

abnormal T cell differentiation ( J:85777 )

• 75% of thymocytes express high levels of CD3 compared to 18% of wild-type thymocytes

• 99% of thymocytes have detectable levels of CD3 compared to only 75% in wild-type thymocytes

• virtually all CD4 T cells in the periphery express the transgenic TCR

abnormal positive T cell selection ( J:85777 )

• the transgenic TCR is positively selected for on this genetic background (H2-E^k) leading to a more efficient positive T cell selection that is skewed towards CD4 T cell production

decreased double-positive T cell number ( J:85777 )

• the percentage of double-positive T cells in the thymus is 24% compared to 83% in wild-type mice

abnormal T cell subpopulation ratio ( J:85777 )

• the CD4/CD8 single positive T cell ratio in the thymus is heavily skewed towards the CD4 population

increased CD4-positive, alpha-beta T cell number ( J:85777 )

• the percentage of CD4 single positive T cells in the thymus is 63% compared to 13% in wild-type mice

• the percentage of CD 4 T cells in lymph nodes is 74% compared to 44% in wild-type controls

decreased CD8-positive, alpha-beta T cell number ( J:85777 )

• there is a two-fold reduction in CD8 single positive T cells in the thymus

• CD8 T cells are severely reduced in peripheral lymph nodes

endocrine/exocrine glands

abnormal thymocyte activation ( J:85777 )

• thymocytes proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells

cellular

abnormal T cell proliferation ( J:85777 )

• T cells proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells

Genotype
MGI:3835451

tg31

• Allelic Composition: Tg(TcrAND)53Hed/?
• Genetic Background: involves: C57BL/6 * SJL

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:124701 )

• the percentage of CD4 single-positive thymocytes is greatly increased

immune system

increased CD4-positive, alpha-beta T cell number ( J:124701 )

• the percentage of CD4 single-positive thymocytes is greatly increased

abnormal lymph node cell ratio ( J:124701 )

• in mice with the H2^k allele, the CD4:CD8 ratio is 15:1 to 100:1 compared to 3:1 in controls