Phenotypes associated with this allele

• Allele Symbol: Noto^tm1Gos
• Allele Name: targeted mutation 1, Achim Gossler
• Allele ID: MGI:3053092
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Noto^tm1Gos/Noto^tm1Gos	involves: 129S1/SvImJ	MGI:3054260
ht2	Noto^tc/Noto^tm1Gos	involves: 129S1/SvImJ * STOCK-Noto^tc/J	MGI:3054259

Genotype
MGI:3054260

hm1

• Allelic Composition: Noto^tm1Gos/Noto^tm1Gos
• Genetic Background: involves: 129S1/SvImJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality ( J:91405 )

• reduced postnatal survival is seen as a result of damage to the spinal cord with only 20% of homozygotes surviving to weaning <

embryo

absent nodal flow ( J:182741 )

• essentially immotile nodal cilia and essentially no directional movement in the nodal flow

truncated notochord ( J:91405 )

• premature truncation of the notochord in the caudal or sacral region with or without a discontinuous caudal notochord is seen

• more anterior defects in the notochord compared to Flh^tc homozygotes may be seen

abnormal primitive node morphology ( J:182741 )

• variable size

• abnormal expression patterns of genes delineating the node

abnormal motile primary cilium morphology ( J:182741 )

• axonemal microtubule structure is disrupted

abnormal embryonic cilium location or orientation ( J:182741 )

• position of cilia emergence is randomized rather than polarized towards the posterior end of the nodal cells

decreased embryonic cilium length ( J:182741 )

• cilia length is reduced at the mid to late head fold stage

limbs/digits/tail

short tail ( J:91405 )

• reduced tail length is seen with more frequently and with greater severity compared to Flh^tc homozygotes

skeleton

decreased vertebrae number ( J:91405 )

• mutants have fewer vertebrae compared to wild-type mice resulting from interruption or premature truncation of the vertebra column

• this phenotype is more frequent and severe compared to Flh^tc homozygotes, however defects are still confined to the tail and sacral region

cellular

abnormal motile primary cilium morphology ( J:182741 )

• axonemal microtubule structure is disrupted

abnormal embryonic cilium location or orientation ( J:182741 )

• position of cilia emergence is randomized rather than polarized towards the posterior end of the nodal cells

decreased embryonic cilium length ( J:182741 )

• cilia length is reduced at the mid to late head fold stage

absent nodal flow ( J:182741 )

• essentially immotile nodal cilia and essentially no directional movement in the nodal flow

Genotype
MGI:3054259

ht2

• Allelic Composition: Noto^tc/Noto^tm1Gos
• Genetic Background: involves: 129S1/SvImJ * STOCK-Noto^tc/J

embryo

truncated notochord ( J:91405 )

• premature truncation of the notochord in the caudal or sacral region with or without a discontinuous caudal notochord is seen

limbs/digits/tail

short tail ( J:91405 )

• reduced tail length, often half the normal length with one or more constrictions along the length of the tail, is seen with incomplete penetrance

skeleton

decreased vertebrae number ( J:91405 )

• mutants have fewer vertebrae compared to wild-type mice resulting from interruption or premature truncation of the vertebra column