Analysis Tools
integument
| N |
• no macroscopic or microscopic skin tumors are observed up to 4 months after tamoxifen treatment
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Allele Symbol Allele Name Allele ID |
Shhtm2(cre/ERT2)Cjt targeted mutation 2, Clifford J Tabin MGI:3053960 |
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| Summary |
10 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• no macroscopic or microscopic skin tumors are observed up to 4 months after tamoxifen treatment
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at E18.5, the respiratory epithelium was populated almost exclusively by Foxj1-expressing ciliated cells, unlike in control lungs where ciliated cells were interspersed with secretory Clara cells
• at E18.5, Ki67 labeling was dramatically reduced in large, medium and small airways, suggesting a severe decrease in epithelial cell proliferation relative to control lungs
• however, goblet cell fate was not lost, as shown by PAS and Alcian Blue staining of tracheal sections at birth (P0)
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• no secretory Clara cells were detected at E18.5, as determined by specific marker analysis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• no epidermal defects or tumor formation are observed with tamoxifen treatment at day 28 (during period of up to 4 months after cre induction) when hair follicles are in full anlagen
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• after 48 hrs of tamoxifen treatment, no tubercle formation is observed at E12.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• larger than in controls
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• mice treated with tamoxifen at E10.5 exhibit increased mitotic index in mesoderm-derived para-cloacal mesenchyme compared with wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• as in Gt(ROSA)26Sortm1Lma/Gt(ROSA)26Sor+ Shhtm2(cre/ERT2)Cjt/Shhtm2(cre/ERT2)Cjt mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• tamoxifen-treated mice exhibit normal masculinization
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• only 1 of 17 pups survived until P28
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• pups failed to thrive and died within 2-3 weeks of life
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• at birth, pups appeared grossly normal but were already smaller than control littermates
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• pups failed to thrive and by P21 were much smaller than control littermates
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| N |
• at E11.5, E14.5 and E18.5, mutant lungs showed normal gross morphology and size relative to control lungs
• no defects in the branching pattern were detected when E11.5 lungs were cultured for 24 and 48 hrs
• distal epithelium differentiation (formation of alveolar sacs and type I and type II cells) remained unaffected
• normal formation of goblet cells was observed in the trachea at birth (P0)
• the distribution and number of basal cells remained normal
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• by P21, isolated foci of inflammatory cells, including macrophages, were seen in the bronchiolar epithelium, unlike in control lungs
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• at P7, P14 and P21, a severely attenuated airway epithelium is noted in medium-sized airways and in terminal bronchioles
• at E18.5, the respiratory epithelium was populated almost exclusively by beta-tubulin-expressing ciliated cells, unlike in control lungs where ciliated cells were interspersed with secretory Clara cells
• at E18.5, Foxj1-positive ciliated cells comprised 80-85% of the population of the airway epithelium, regardless of the airway generation, unlike in control lungs where Foxj1-expressing cells averaged 40%, 18% and 17% of epithelial cells seen in the large, medium and small airways, respectively
• at E18.5, Ki67 labeling averaged 15%, 10% and 5% of control values in large, medium and small airways, respectively, suggesting a substantial decrease in epithelial cell proliferation
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• by P21, a thin metaplastic squamous epithelium, scattered cell debris and isolated macrophages were observed instead of the typical cuboidal epithelium seen in control bronchioles
• however, none of these changes were apparent at birth or at E18.5
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• no secretory Clara cells were detected at E18.5 and P0, as determined by specific marker expression analysis
• however, no differences in apoptosis were noted at E14.5, E18.5 and P0 lungs
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• by P21, isolated foci of inflammatory cells, including macrophages, were seen in the bronchiolar epithelium, unlike in control lungs
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• at E18.5, the number of neuroendocrine cells is significantly increased in mutant airways, as shown by Pgp9.5
immunostaining
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• at E18.5, the number of neuroendocrine cells is significantly increased in mutant airways, as shown by Pgp9.5
immunostaining
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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