Phenotypes associated with this allele

• Allele Symbol: Shh^{tm2(cre/ERT2)Cjt}
• Allele Name: targeted mutation 2, Clifford J Tabin
• Allele ID: MGI:3053960
• Summary: 10 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Kras^tm4Tyj/Kras^+ Trp53^tm1Brn/Trp53^tm1Brn Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129P2/OlaHsd * 129S4/SvJae * 129S6/SvEvTac	MGI:5298088
cn2	Rbpj^tm1Hon/Rbpj^tm1.1Hon Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129P2/OlaHsd * 129S6/SvEvTac	MGI:4948663
cn3	Kras^tm4Tyj/Kras^+ Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129S4/SvJae * 129S6/SvEvTac	MGI:5298087
cn4	Gt(ROSA)26Sor^tm1(Notch1)Dam/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129S4/SvJaeSor * 129S6/SvEvTac	MGI:3718107
cn5	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^tm2(cre/ERT2)Cjt	involves: 129S6/SvEvTac	MGI:5694214
cn6	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129S6/SvEvTac	MGI:5694209
cn7	Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc/Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129S6/SvEvTac * 129X1/SvJ	MGI:3810321
cn8	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^tm2(cre/ERT2)Cjt Tg(Msx2-cre)5Rem/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:5694215
cn9	Ar^tm2Ska/Y Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:4461253
cn10	Pofut1^tm2Pst/Pofut1^tm1Pst Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129/Sv * 129S6/SvEvTac * C57BL/6J * SJL	MGI:4948658

Genotype
MGI:5298088

cn1

• Allelic Composition: Kras^tm4Tyj/Kras⁺; Trp53^tm1Brn/Trp53^tm1Brn; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

integument phenotype ( J:172048 )

N • no macroscopic or microscopic skin tumors are observed up to 4 months after tamoxifen treatment

Genotype
MGI:4948663

cn2

• Allelic Composition: Rbpj^tm1Hon/Rbpj^tm1.1Hon; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac

respiratory system

abnormal respiratory epithelium morphology ( J:150051 )

• at E18.5, the respiratory epithelium was populated almost exclusively by Foxj1-expressing ciliated cells, unlike in control lungs where ciliated cells were interspersed with secretory Clara cells

• at E18.5, Ki67 labeling was dramatically reduced in large, medium and small airways, suggesting a severe decrease in epithelial cell proliferation relative to control lungs

• however, goblet cell fate was not lost, as shown by PAS and Alcian Blue staining of tracheal sections at birth (P0)

absent club cells ( J:150051 )

• no secretory Clara cells were detected at E18.5, as determined by specific marker analysis

Genotype
MGI:5298087

cn3

• Allelic Composition: Kras^tm4Tyj/Kras⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129S4/SvJae * 129S6/SvEvTac

integument

integument phenotype ( J:172048 )

N • no epidermal defects or tumor formation are observed with tamoxifen treatment at day 28 (during period of up to 4 months after cre induction) when hair follicles are in full anlagen

Genotype
MGI:3718107

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Notch1)Dam}/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac

vision/eye

vision/eye phenotype ( J:118372 )

N • retina size is normal

Genotype
MGI:5694214

cn5

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh^{tm2(cre/ERT2)Cjt}
• Genetic Background: involves: 129S6/SvEvTac

reproductive system

abnormal genital tubercle morphology ( J:223057 )

• after 48 hrs of tamoxifen treatment, no tubercle formation is observed at E12.5

Genotype
MGI:5694209

cn6

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129S6/SvEvTac

reproductive system

abnormal genital tubercle morphology ( J:223057 )

• larger than in controls

abnormal reproductive system physiology ( J:223057 )

• mice treated with tamoxifen at E10.5 exhibit increased mitotic index in mesoderm-derived para-cloacal mesenchyme compared with wild-type mice

Genotype
MGI:3810321

cn7

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}/Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129S6/SvEvTac * 129X1/SvJ

neoplasm

neoplasm ( J:139574 )

N • following induction with tamoxifen, mice do not exhibit medulloblastoma

Genotype
MGI:5694215

cn8

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh^{tm2(cre/ERT2)Cjt}; Tg(Msx2-cre)5Rem/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

limbs/digits/tail

abnormal limb development ( J:223057 )

• as in Gt(ROSA)26Sor^tm1Lma/Gt(ROSA)26Sor⁺ Shh^{tm2(cre/ERT2)Cjt}/Shh^{tm2(cre/ERT2)Cjt} mice

Genotype
MGI:4461253

cn9

• Allelic Composition: Ar^tm2Ska/Y; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

reproductive system

reproductive system phenotype ( J:148515 )

♂N • tamoxifen-treated mice exhibit normal masculinization

Genotype
MGI:4948658

cn10

• Allelic Composition: Pofut1^tm2Pst/Pofut1^tm1Pst; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129/Sv * 129S6/SvEvTac * C57BL/6J * SJL

mortality/aging

premature death ( J:150051 )

• only 1 of 17 pups survived until P28

postnatal lethality, incomplete penetrance ( J:150051 )

• pups failed to thrive and died within 2-3 weeks of life

growth/size/body

decreased birth body size ( J:150051 )

• at birth, pups appeared grossly normal but were already smaller than control littermates

decreased body size ( J:150051 )

• pups failed to thrive and by P21 were much smaller than control littermates

respiratory system

respiratory system phenotype ( J:150051 )

N • at E11.5, E14.5 and E18.5, mutant lungs showed normal gross morphology and size relative to control lungs

N • no defects in the branching pattern were detected when E11.5 lungs were cultured for 24 and 48 hrs

N • distal epithelium differentiation (formation of alveolar sacs and type I and type II cells) remained unaffected

N • normal formation of goblet cells was observed in the trachea at birth (P0)

N • the distribution and number of basal cells remained normal

lung inflammation ( J:150051 )

• by P21, isolated foci of inflammatory cells, including macrophages, were seen in the bronchiolar epithelium, unlike in control lungs

abnormal respiratory epithelium morphology ( J:150051 )

• at P7, P14 and P21, a severely attenuated airway epithelium is noted in medium-sized airways and in terminal bronchioles

• at E18.5, the respiratory epithelium was populated almost exclusively by beta-tubulin-expressing ciliated cells, unlike in control lungs where ciliated cells were interspersed with secretory Clara cells

• at E18.5, Foxj1-positive ciliated cells comprised 80-85% of the population of the airway epithelium, regardless of the airway generation, unlike in control lungs where Foxj1-expressing cells averaged 40%, 18% and 17% of epithelial cells seen in the large, medium and small airways, respectively

• at E18.5, Ki67 labeling averaged 15%, 10% and 5% of control values in large, medium and small airways, respectively, suggesting a substantial decrease in epithelial cell proliferation

abnormal bronchiole epithelium morphology ( J:150051 )

• by P21, a thin metaplastic squamous epithelium, scattered cell debris and isolated macrophages were observed instead of the typical cuboidal epithelium seen in control bronchioles

• however, none of these changes were apparent at birth or at E18.5

absent club cells ( J:150051 )

• no secretory Clara cells were detected at E18.5 and P0, as determined by specific marker expression analysis

• however, no differences in apoptosis were noted at E14.5, E18.5 and P0 lungs

immune system

lung inflammation ( J:150051 )

• by P21, isolated foci of inflammatory cells, including macrophages, were seen in the bronchiolar epithelium, unlike in control lungs

nervous system

increased solitary pulmonary neuroendocrine cell number ( J:150051 )

• at E18.5, the number of neuroendocrine cells is significantly increased in mutant airways, as shown by Pgp9.5 immunostaining

endocrine/exocrine glands

increased solitary pulmonary neuroendocrine cell number ( J:150051 )

• at E18.5, the number of neuroendocrine cells is significantly increased in mutant airways, as shown by Pgp9.5 immunostaining