Phenotypes associated with this allele

• Allele Symbol: Tg(DBHn-lacZ)8Rpk
• Allele Name: transgene insertion 8, Raj P Kapur
• Allele ID: MGI:3055106
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Hlx^tm1Rph/Hlx^tm1Rph Tg(DBHn-lacZ)8Rpk/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL	MGI:3773359
cx2	Sox10^Dom/Sox10^+ Tg(DBHn-lacZ)8Rpk/0	involves: C3HeB/FeJLe * C57BL/6JLe * SJL	MGI:5897678

Genotype
MGI:3773359

cx1

• Allelic Composition: Hlx^tm1Rph/Hlx^tm1Rph; Tg(DBHn-lacZ)8Rpk/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal enteric neuron morphology ( J:119681 )

• the number and distribution of enteric neuronal precursors/neurons is altered such that the neural crest cells/enteric neurons remain restricted to the lateral stomach mesenchyme and only a few scattered cells in the intestine are seen between E10.5-E16.5

Genotype
MGI:5897678

cx2

• Allelic Composition: Sox10^Dom/Sox10⁺; Tg(DBHn-lacZ)8Rpk/0
• Genetic Background: involves: C3HeB/FeJLe * C57BL/6JLe * SJL

digestive/alimentary system

abnormal intestine morphology ( J:32868 )

• Background Sensitivity: phenotype is more severe when crossed to C57BL/6J females with all offspring completely lacking ganglion cells in the distal large intestine compared to only 1/3 of offspring from crosses to C3HeB/FeJ females

• Background Sensitivity: in crosses to C3HeB/FeJ females the other 2/3 of offspring have reduced numbers of ganglion or no detectable changes in ganglion numbers

• hypoganglionosis without aganglionosis is confined to the distal 1-2 cm of the adult colon

abnormal colon morphology ( J:32868 )

• hypoganglionosis without aganglionosis is confined to the distal 1-2 cm of the adult colon

abnormal rectum morphology ( J:32868 )

• aganglionosis or hypoganglionosis always involves the distal rectum

integument

white spotting ( J:32868 )

• almost all have one or more white paws

• more than half have ventral white spots

cellular

abnormal vagal neural crest cell migration ( J:32868 )

• delayed colonization of the intestine by vagal neural crest cells between E11.0 to E13.5

• reduced density of neuroblasts in the intestine between E11.0 to E13.5 especially near the migration front

nervous system

abnormal parasympathetic ganglion morphology ( J:32868 )

• almost all display aganglionosis or hypoganglionosis

• Background Sensitivity: phenotype is more severe when crossed to C57BL/6J females with all offspring completely lacking ganglion cells in the distal large intestine compared to only 1/3 of offspring from crosses to C3HeB/FeJ females

• Background Sensitivity: in crosses to C3HeB/FeJ females the other 2/3 of offspring have reduced numbers of ganglion or no detectable changes in ganglion numbers

• aganglionic segements of the large intestine lack both myenteric and submucosal ganglion cells

embryo

abnormal vagal neural crest cell migration ( J:32868 )

• delayed colonization of the intestine by vagal neural crest cells between E11.0 to E13.5

• reduced density of neuroblasts in the intestine between E11.0 to E13.5 especially near the migration front

pigmentation

white spotting ( J:32868 )

• almost all have one or more white paws

• more than half have ventral white spots