Phenotypes associated with this allele

• Allele Symbol: Rasgrp2^tm1Amg
• Allele Name: targeted mutation 1, Ann M Graybiel
• Allele ID: MGI:3055135
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rasgrp2^tm1Amg/Rasgrp2^tm1Amg	Not Specified	MGI:3055194
cx2	Rasa3^hlb381/Rasa3^hlb381 Rasgrp2^tm1Amg/Rasgrp2^tm1Amg	involves: C57BL/6J	MGI:6378569

Genotype
MGI:3055194

hm1

• Allelic Composition: Rasgrp2^tm1Amg/Rasgrp2^tm1Amg
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal neutrophil physiology ( J:122618 )

• neutrophils from homozygous mutant mice exhibit strong defects in Rap1 and beta1 and beta2 integrin activation while maintaining normal calcium flux, degranulation, and ROS generation

impaired neutrophil recruitment ( J:122618 )

• neutrophils from homozygous mutant mice failed to adhere firmly to stimulated venules and to migrate into sites of inflammation

abnormal platelet physiology ( J:92864 )

• platelets are unable to form stable thrombi when stimulated by collagen under flow conditions and mutants are resistant to collagen-induced thrombi mediated mortali

decreased platelet aggregation ( J:92864 , J:122618 )

• aggregation of platelets in response to calcium ionophore, collagen, ADP and a thromboxane A analog is impaired (J:92864)

• deficiency caused complete inhibition of arterial thrombus formation in mice (J:122618)

immune system

abnormal neutrophil physiology ( J:122618 )

• neutrophils from homozygous mutant mice exhibit strong defects in Rap1 and beta1 and beta2 integrin activation while maintaining normal calcium flux, degranulation, and ROS generation

impaired neutrophil recruitment ( J:122618 )

• neutrophils from homozygous mutant mice failed to adhere firmly to stimulated venules and to migrate into sites of inflammation

homeostasis/metabolism

abnormal platelet physiology ( J:92864 )

• platelets are unable to form stable thrombi when stimulated by collagen under flow conditions and mutants are resistant to collagen-induced thrombi mediated mortali

decreased platelet aggregation ( J:92864 , J:122618 )

• aggregation of platelets in response to calcium ionophore, collagen, ADP and a thromboxane A analog is impaired (J:92864)

• deficiency caused complete inhibition of arterial thrombus formation in mice (J:122618)

increased bleeding time ( J:92864 )

• after tail transection mutants bleed for at least 30 minutes compared to less than 8 minutes for wild-type mice

• mutants do not develop spontaneous hemorrhage or anemia

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
leukocyte adhesion deficiency 3		DOID:0110912	OMIM:612840	J:122618

Genotype
MGI:6378569

cx2

• Allelic Composition: Rasa3^hlb381/Rasa3^hlb381; Rasgrp2^tm1Amg/Rasgrp2^tm1Amg
• Genetic Background: involves: C57BL/6J

hematopoietic system

abnormal platelet cell number ( J:221899 )

• loss of RASGRP2 counterbalances the loss of RASA3 and the number of platelets is increased compared with RASA3 mutants, although still lower than normal, and the half life of platelets is longer than that of RASA3 mutants, although not normal

homeostasis/metabolism

abnormal hemostasis ( J:221899 )

• hemostatic plug formation is improved relative to RASA3 mutants lacking RASGRP2 ablation, although compared with wild-type controls the platelet accumulation and hemostatic plug formation is slower but also more stable than normal