About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Btrctm1Paga
targeted mutation 1, Michele Pagano
MGI:3055737
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Btrctm1Paga/Btrctm1Paga involves: 129S2/SvPas MGI:3056062
cn2
Btrctm1Paga/Btrctm1Paga
Fbxw11tm1Ybn/Fbxw11tm1Ybn
Tg(Vil1-cre/ERT2)23Syr/?
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6 * DBA/2 MGI:5568951
cn3
Btrctm1Paga/Btrc+
Fbxw11tm1Ybn/Fbxw11tm1Ybn
Tg(Vil1-cre/ERT2)23Syr/?
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6 * DBA/2 MGI:5568952


Genotype
MGI:3056062
hm1
Allelic
Composition
Btrctm1Paga/Btrctm1Paga
Genetic
Background
involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Btrctm1Paga mutation (2 available); any Btrc mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• homozygous mice appeared generally as healthy as wildtype littermates over 2 years' observation
• almost all mutants revealed no gross tissue abnormalities upon necropsy
• exceptions were a single invasive intestinal carcinoma in a 10 month-old mouse and death due to thymic lymphoma of 2 mice aged 6.5 and 9 months

cellular
• histological examination of epidydimes of homozygous males revealed severely diminished numbers of mature spermatozoa
• approximately 20% of asynchronously dividing MEFs were found to have supernumerary centrosomes
• histological examination of epidydimes of homozygous males revealed evidence of prolonged, abnormal meiosis
• numbers of metaphase I spermatocytes were elevated
• in some mice, spindle abnormalities and misaligned chromosomes were observed
• the degree of fertility impairment correlated with the severity of histologic abnormality
• in some spermatocytes, spindle abnormalities and misaligned chromosomes were observed
• cultured homozygous mouse embryonic fibroblasts (MEFs) were retarded in their progression through mitosis, despite normal passage from G1 to S phase
• MEFs exhibited misalignment of chromosomes at the metaphase plate
• approximately 20% of asynchronously dividing MEFs were found to have supernumerary centrosomes, and approximately 12% of dividing MEFs exhibited multipolar spindles
• approximately 12% of dividing MEFs exhibited multipolar spindles

reproductive system
• histological examination of epidydimes of homozygous males revealed severely diminished numbers of mature spermatozoa
• histological examination of epidydimes of homozygous males revealed evidence of prolonged, abnormal meiosis
• numbers of metaphase I spermatocytes were elevated
• in some mice, spindle abnormalities and misaligned chromosomes were observed
• the degree of fertility impairment correlated with the severity of histologic abnormality
• in some spermatocytes, spindle abnormalities and misaligned chromosomes were observed
• approximately 50% of homozygous male mice produced no progeny when paired with wild-type females
• fertility defect was not behavioral in origin, as mating behavior was normal and vaginal plugs were observed
• productive homozygous males sired fewer and smaller litters, on average, than did wild-type males




Genotype
MGI:5568951
cn2
Allelic
Composition
Btrctm1Paga/Btrctm1Paga
Fbxw11tm1Ybn/Fbxw11tm1Ybn
Tg(Vil1-cre/ERT2)23Syr/?
Genetic
Background
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Btrctm1Paga mutation (2 available); any Btrc mutation (46 available)
Fbxw11tm1Ybn mutation (0 available); any Fbxw11 mutation (72 available)
Tg(Vil1-cre/ERT2)23Syr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• appearance of severe diarrhea and rectal bleeding in mice given tamoxifen is followed by death within 24 hours
• appearance of severe diarrhea and rectal bleeding in mice given tamoxifen is followed by death within 24 hours
• wide gaps in epithelial tight junctions found in enterocytes following tamoxifen administration
• loss of most epithelial cells in large intestine following tamoxifen administration
• remaining mucosa is infiltrated with inflammatory and immune cells
• intestinal barrier disruption observed as early as 24 hours after tamoxifen treatment
• unrecognizable crypt structures in large intestine by day 5 following tamoxifen administration
• mice develop severe colitis following tamoxifen administration
• loss of most epithelial cells in large intestine following tamoxifen administration
• remaining mucosa is infiltrated with inflammatory and immune cells
• inflammation in small and large intestines following tamoxifen administration

cellular
• more than 2 centrosomes observed in enterocytes following tamoxifen administration
• mitosis is abnormally prolonged in enterocytes following tamoxifen administration
• mitotic enterocytes have abnormal spindles following tamoxifen administration
• observed in enterocytes following tamoxifen administration

cardiovascular system
• appearance of severe diarrhea and rectal bleeding in mice given tamoxifen is followed by death within 24 hours

endocrine/exocrine glands
• unrecognizable crypt structures in large intestine by day 5 following tamoxifen administration

homeostasis/metabolism
• increase in IL1b levels beginning day 1 and spiking on day 3 following tamoxifen administration

immune system
• inflammation in small and large intestines following tamoxifen administration
• increase in IL1b levels beginning day 1 and spiking on day 3 following tamoxifen administration

mortality/aging
• appearance of severe diarrhea and rectal bleeding in mice given tamoxifen is followed by death within 24 hours




Genotype
MGI:5568952
cn3
Allelic
Composition
Btrctm1Paga/Btrc+
Fbxw11tm1Ybn/Fbxw11tm1Ybn
Tg(Vil1-cre/ERT2)23Syr/?
Genetic
Background
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Btrctm1Paga mutation (2 available); any Btrc mutation (46 available)
Fbxw11tm1Ybn mutation (0 available); any Fbxw11 mutation (72 available)
Tg(Vil1-cre/ERT2)23Syr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• severe inflammation and a disrupted epithelial layer occurs 4 days after tamoxifen administration
• wide gaps in enterocyte epithelial tight junctions following tamoxifen administration
• mucositis observed in colon following tamoxifen administration
• spontaneous recovery occurs 3 weeks after tamoxifen administration





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory