All Phenotypes Tg(Gdf9-icre)5092Coo MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(Gdf9-icre)5092Coo
transgene insertion 5092, Austin J Cooney
MGI:3056522

Summary

22 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gdf9-icre)5092Coo/0	B6.Cg-Pten^tm1Hwu Tg(Gdf9-icre)5092Coo	MGI:4839213
cn2	Kmt2b^tm1Afst/Kmt2b^tm1.1Afst Tg(Gdf9-icre)5092Coo/0	involves: 129 * 129P2/OlaHsd * C57BL/6	MGI:4867493
cn3	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^tm2(DTA)Riet Tg(Gdf9-icre)5092Coo/0	involves: 129P2/OlaHsd * C57BL/6	MGI:4430336
cn4	Furin^tm1Jwmc/Furin^tm1Jwmc Tg(Gdf9-icre)5092Coo/0	involves: 129P2/OlaHsd * C57BL/6J * CBA/J	MGI:6856674
cn5	Setd1a^tm1.1Afst/Setd1a^tm1.2Afst Tg(Gdf9-icre)5092Coo/0	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6	MGI:5568949
cn6	Setd1a^tm1.1Afst/Setd1a^tm1.1Afst Tg(Gdf9-icre)5092Coo/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5568948
cn7	Kat8^tm1Thl/Kat8^tm1Thl Tg(Gdf9-icre)5092Coo/0	involves: 129S1/Sv * C57BL/6	MGI:6852522
cn8	Csnk2b^tm1.1Bb/Csnk2b^tm1.1Bb Tg(Gdf9-icre)5092Coo/0	involves: 129S2/SvPasCrl * C57BL/6	MGI:6845081
cn9	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gdf9-icre)5092Coo/0	involves: 129S4/SvJae * C57BL/6	MGI:4882032
cn10	Pten^tm1Hwu/Pten^tm1Hwu Tsc1^tm1Djk/Tsc1^tm1Djk Tg(Gdf9-icre)5092Coo/?	involves: 129S4/SvJae * C57BL/6J	MGI:4941741
cn11	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gdf9-icre)5092Coo/?	involves: 129S4/SvJae * C57BL/6J	MGI:4941739
cn12	Tsc1^tm1Djk/Tsc1^tm1Djk Tg(Gdf9-icre)5092Coo/?	involves: 129S4/SvJae * C57BL/6J	MGI:4941735
cn13	Pdpk1^tm1Maka/Pdpk1^tm1Maka Tsc1^tm1Djk/Tsc1^tm1Djk Tg(Gdf9-icre)5092Coo/?	involves: 129S4/SvJae * C57BL/6J	MGI:4941744
cn14	Smc1b^tm1Jess/Smc1b^tm2.1Jess Tg(Gdf9-icre)5092Coo/0	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:4867233
cn15	Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/Gt(ROSA)26Sor^{tm1(HBEGF)Awai} Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6	MGI:4430335
cn16	Dcaf13^em2Hyfn/Dcaf13^em2Hyfn Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6	MGI:7314401
cn17	Rps26^tm1.1Jzc/Rps26^tm1.1Jzc Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6 * C57BL/6J	MGI:6712243
cn18	Chd7^{tm2c(EUCOMM)Wtsi}/Chd7^{tm2c(EUCOMM)Wtsi} Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6 * C57BL/6J * C57BL/6N	MGI:7311617
cn19	Aurka^{tm1c(EUCOMM)Hmgu}/Aurka^{tm1c(EUCOMM)Hmgu} Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6 * C57BL/6J * C57BL/6N	MGI:7284072
cn20	Rtcb^tm1Hyfn/Rtcb^tm1Hyfn Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6J	MGI:7620017
cn21	Smc1b^tm2.1Jess/Smc1b^tm2.1Jess Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6 * SJL	MGI:4867232
cn22	Foxl2^tm2.1Tre/Foxl2^tm2.1Tre Tg(Gdf9-icre)5092Coo/0	involves: C57BL/6 * SJL	MGI:4430334

Allelic Composition	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gdf9-icre)5092Coo/0
Genetic Background	B6.Cg-Pten^tm1Hwu Tg(Gdf9-icre)5092Coo

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pten^tm1Hwu mutation (16 available); any Pten mutation (88 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal oocyte morphology ( J:131827 )

• accelerated oocyte growth

• many transient follicles contain degraded oocytes

growth/size/body

enlarged ovary ( J:131827 )

• at P8, ovaries appear larger with more activated follicles

• by P23 and 35, ovaries remain larger and contain more activated follicles

endocrine/exocrine glands

abnormal corpus luteum morphology ( J:131827 )

• luteolysis is observed (degeneration of corpora lutea) at 12 weeks of age

abnormal ovarian follicle morphology ( J:131827 )

• at P8, ovaries appear larger with more activated follicles, including transient follicles containing enlarged oocytes surrounded by flattened pregranulosa cells, primary follicles with enlarged oocytes surrounded by one layer of cuboidal granulosa cells and some secondary follicles with two layers of granulosa cells

• follicular structure at 12 weeks of age is deformed and luteolysis is observed

• reduction in follicle death and clearance before and around the time of sexual maturity

• at 7 weeks of age, ovaries show increased numbers of transient and preantral type 5 follicles

abnormal ovarian folliculogenesis ( J:131827 )

• growth dynamics of activated transient follicles is different than in controls, with some follicles remaining at the transient stage whereas others develop further

abnormal primordial ovarian follicle morphology ( J:131827 )

• premature activation of the primordial follicle pool resulting in depletion of primordial follicles in early adulthood

decreased primordial ovarian follicle number ( J:131827 )

• percentage of primordial follicles in ovaries at P8 is lower (49.6%) than in controls (83.6%)

absent primordial ovarian follicles ( J:131827 )

• by P23, no primordial follicles can be identified in mutants compared to 69.2% of follicles in controls areas are still at the primordial stage

enlarged ovary ( J:131827 )

• at P8, ovaries appear larger with more activated follicles

• by P23 and 35, ovaries remain larger and contain more activated follicles

premature ovarian failure ( J:131827 )

• females exhibit activation of the pool of primordial follicles that leads to follicle depletion, causing premature ovarian failure

• many transient follicles contain degraded oocytes suggesting that some prematurely activated follicles undergo atresia

homeostasis/metabolism

increased follicle stimulating hormone level ( J:131827 )

• increase in levels of follicle-stimulating hormone at 12-20-week old females

increased luteinizing hormone level ( J:131827 )

• increase in levels of luteinizing hormone at 12-20-week old females

mortality/aging

premature ovarian failure ( J:131827 )

• females exhibit activation of the pool of primordial follicles that leads to follicle depletion, causing premature ovarian failure

• many transient follicles contain degraded oocytes suggesting that some prematurely activated follicles undergo atresia

reproductive system

abnormal oocyte morphology ( J:131827 )

• accelerated oocyte growth

• many transient follicles contain degraded oocytes

abnormal corpus luteum morphology ( J:131827 )

• luteolysis is observed (degeneration of corpora lutea) at 12 weeks of age

abnormal ovarian follicle morphology ( J:131827 )

• follicular structure at 12 weeks of age is deformed and luteolysis is observed

• reduction in follicle death and clearance before and around the time of sexual maturity

• at 7 weeks of age, ovaries show increased numbers of transient and preantral type 5 follicles

abnormal ovarian folliculogenesis ( J:131827 )

• growth dynamics of activated transient follicles is different than in controls, with some follicles remaining at the transient stage whereas others develop further

abnormal primordial ovarian follicle morphology ( J:131827 )

• premature activation of the primordial follicle pool resulting in depletion of primordial follicles in early adulthood

decreased primordial ovarian follicle number ( J:131827 )

• percentage of primordial follicles in ovaries at P8 is lower (49.6%) than in controls (83.6%)

absent primordial ovarian follicles ( J:131827 )

• by P23, no primordial follicles can be identified in mutants compared to 69.2% of follicles in controls areas are still at the primordial stage

enlarged ovary ( J:131827 )

• at P8, ovaries appear larger with more activated follicles

• by P23 and 35, ovaries remain larger and contain more activated follicles

premature ovarian failure ( J:131827 )

• females exhibit activation of the pool of primordial follicles that leads to follicle depletion, causing premature ovarian failure

• many transient follicles contain degraded oocytes suggesting that some prematurely activated follicles undergo atresia

abnormal estrous cycle ( J:131827 )

• older females exhibit irregular estrous cycles

female infertility ( J:131827 )

• females become infertile in early adulthood, after 12-13 weeks of age

Allelic Composition	Kmt2b^tm1Afst/Kmt2b^tm1.1Afst Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129 * 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kmt2b^tm1.1Afst mutation (0 available); any Kmt2b mutation (65 available)
Kmt2b^tm1Afst mutation (0 available); any Kmt2b mutation (65 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal female meiosis I arrest ( J:166778 )

• a small but significant fraction remains arrested in meiosis I in cultured peri-ovulatory oocytes for in vitro oocyte maturation studies

abnormal ovarian follicle number ( J:166778 )

• very few follicles at 36 weeks old

increased primary ovarian follicle number ( J:166778 )

• a higher number of primary growing follicles at 2 weeks old

decreased primordial ovarian follicle number ( J:166778 )

• a 50% decrease in primordial follicles at 3 weeks old

• an 80% reduction in primordial follicles at 8 weeks old

increased secondary ovarian follicle number ( J:166778 )

• increase in growing preantral follicles at 3 weeks old

increased atretic ovarian follicle number ( J:166778 )

• increased number of atretic follicles from 3 week of age onwards

increased follicle recruitment ( J:166778 )

• a higher number of primary growing follicles at 2 weeks old

• a 50% decrease in primordial follicles and an increase in growing preantral follicles at 3 weeks old

• an 80% reduction in primordial follicles at 8 weeks old

• increased number of atretic follicles from 3 week of age onwards

• very few follicles at 36 weeks old

decreased superovulation rate ( J:166778 )

• reduced ovulation rates in 3-week-old superovulated females

• trapped oocytes in luteinizing structures in hormonally stimulated females

female infertility ( J:166778 )

homeostasis/metabolism

decreased circulating estradiol level ( J:166778 )

• decreased serum estradiol levels at 8 weeks old

increased circulating follicle stimulating hormone level ( J:166778 )

• increased serum FSH levels at 8 weeks old

increased circulating luteinizing hormone level ( J:166778 )

• increased serum LH levels at 8 weeks old

cellular

abnormal cell physiology ( J:166778 )

• increased H4K12 acetylation

abnormal female meiosis I arrest ( J:166778 )

• a small but significant fraction remains arrested in meiosis I in cultured peri-ovulatory oocytes for in vitro oocyte maturation studies

abnormal DNA methylation ( J:166778 )

• increased mono-methylated H3K4 in oocytes

• decreased bi- and tri-methylated H3K4 in oocytes

abnormal imprinting ( J:166778 )

• fail to establish transcriptional repression

• 80% of the oocytes are still transcriptionally active at the peri-ovulatory stage

• normal nucleolus configuration

endocrine/exocrine glands

abnormal ovarian follicle number ( J:166778 )

• very few follicles at 36 weeks old

increased primary ovarian follicle number ( J:166778 )

• a higher number of primary growing follicles at 2 weeks old

decreased primordial ovarian follicle number ( J:166778 )

• a 50% decrease in primordial follicles at 3 weeks old

• an 80% reduction in primordial follicles at 8 weeks old

increased secondary ovarian follicle number ( J:166778 )

• increase in growing preantral follicles at 3 weeks old

increased atretic ovarian follicle number ( J:166778 )

• increased number of atretic follicles from 3 week of age onwards

increased follicle recruitment ( J:166778 )

• a higher number of primary growing follicles at 2 weeks old

• a 50% decrease in primordial follicles and an increase in growing preantral follicles at 3 weeks old

• an 80% reduction in primordial follicles at 8 weeks old

• increased number of atretic follicles from 3 week of age onwards

• very few follicles at 36 weeks old

Allelic Composition	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^tm2(DTA)Riet Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^tm2(DTA)Riet mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

absent oocytes ( J:157008 )

• no oocytes are observed in ovaries of 8-week old females

mortality/aging

mortality/aging ( J:157008 )

N	• mice are viable

reproductive system

absent oocytes ( J:157008 )

• no oocytes are observed in ovaries of 8-week old females

Allelic Composition	Furin^tm1Jwmc/Furin^tm1Jwmc Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6J * CBA/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Furin^tm1Jwmc mutation (0 available); any Furin mutation (48 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased granulosa cell proliferation ( J:272231 )

• PCNA staining showed a severe defect in granulosa cell proliferation at 2 months of age

increased secondary ovarian follicle number ( J:272231 )

• number of early secondary follicles is significantly increased at 6 weeks of age

impaired ovarian folliculogenesis ( J:272231 )

• ovarian follicle development is arrested at the early secondary follicle stage

• however, number of primordial follicles is normal at 2 months of age, suggesting that survival and activation of primordial follicles is unaffected

female infertility ( J:272231 )

cellular

decreased granulosa cell proliferation ( J:272231 )

• PCNA staining showed a severe defect in granulosa cell proliferation at 2 months of age

endocrine/exocrine glands

decreased granulosa cell proliferation ( J:272231 )

• PCNA staining showed a severe defect in granulosa cell proliferation at 2 months of age

increased secondary ovarian follicle number ( J:272231 )

• number of early secondary follicles is significantly increased at 6 weeks of age

impaired ovarian folliculogenesis ( J:272231 )

• ovarian follicle development is arrested at the early secondary follicle stage

• however, number of primordial follicles is normal at 2 months of age, suggesting that survival and activation of primordial follicles is unaffected

Allelic Composition	Setd1a^tm1.1Afst/Setd1a^tm1.2Afst Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Setd1a^tm1.1Afst mutation (0 available); any Setd1a mutation (57 available)
Setd1a^tm1.2Afst mutation (0 available); any Setd1a mutation (57 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

embryonic growth retardation ( J:208420 )

• at E6.5 and E7.5

growth/size/body

embryonic growth retardation ( J:208420 )

• at E6.5 and E7.5

Allelic Composition	Setd1a^tm1.1Afst/Setd1a^tm1.1Afst Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Setd1a^tm1.1Afst mutation (0 available); any Setd1a mutation (57 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:207420 )

N	• normal litter size and number of litters

Allelic Composition	Kat8^tm1Thl/Kat8^tm1Thl Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129S1/Sv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kat8^tm1Thl mutation (0 available); any Kat8 mutation (38 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal oogenesis ( J:242000 )

• pregnant mare serum gonadotropin (PMSG)-primed females show abnormal oogenesis at 8 weeks of age

abnormal oocyte morphology ( J:242000 )

• after PMSG priming, germinal vesicle (GV) stage oocytes are much smaller (mean diameter 63.7 um versus 81.4 um in wild-types oocytes) and show abnormal morphology, including cytoplasmic granulation, shrinkage and an irregular shape

• at 3 weeks of age, the ratio of oocytes with >5 gamma-H2AX foci is ~56.8% versus ~14.6% in wild-type oocytes, indicating severe DNA damage (double-strand breaks)

• oocytes from 2-week-old females show abnormal heterochromatin configurations: >55% of heterochromatin regions are abnormally shaped, unlike in wild-type oocytes where >80% of heterochromatin regions display a normal oval or round shape

• NAC-treated oocytes show a 58.7% reduction in double-strand breaks relative to PBS-treated oocytes

decreased oocyte number ( J:242000 )

• after PMSG priming, only ~20 full-grown germinal vesicle (GV) stage oocytes are recovered per female versus ~50 oocytes per wild-type control

• following superovulation with PMSG and HCG, more metaphase II (MII) oocytes are recovered from NAC-treated mice than from PBS-treated mice at 3 weeks of age

abnormal female germ cell physiology ( J:242000 )

• oocytes from 2-week-old females show decreased antioxidant gene expression and increased reactive oxygen species (ROS) levels

• following daily i.p. injection of antioxidant N-acetylcysteine (NAC) for 7 days, NAC-treated oocytes show a 67.1% reduction in ROS levels relative to PBS-treated oocytes

increased female germ cell apoptosis ( J:242000 )

• TUNEL assays revealed significant oocyte apoptosis at 3 weeks of age (~17.2% versus ~5.7% in wild-type oocytes)

• following daily i.p. injection of antioxidant N-acetylcysteine (NAC) for 7 days, NAC-treated oocytes show a 56.1% reduction in TUNEL+ cells

increased atretic ovarian follicle number ( J:242000 )

• ovaries contain 2-fold more atretic follicles at 3 weeks of age

• NAC-treated ovaries show a 54.7% reduction in the number of atretic follicles relative to PBS-treated ovaries

impaired ovarian folliculogenesis ( J:242000 )

• ovaries contain 50.4%, 49.6% and 78.7% fewer secondary, preantral and antral follicles at 3 weeks of age

• ovarian follicle development arrests in the secondary and preantral follicle stage; however, granulosa cell proliferation is normal in secondary and preantral follicles at 2 weeks of age

• TUNEL assays and gamma-H2AX immunostaining confirmed that oocytes undergoing apoptosis or exhibiting severe DNA damage are localized in secondary and preantral follicles, but not in primordial or primary follicles

• NAC-treated ovaries show a 60.0%, 83.8% and 224.2% increase in the number of secondary, preantral and antral follicles, respectively, indicating partial rescue of follicular development and survival

small ovary ( J:242000 )

• ovaries are markedly smaller at 8 weeks of age

• following daily i.p. injection of antioxidant N-acetylcysteine (NAC) for 7 days, 3-week-old NAC-treated mice have larger ovaries than PBS-treated mice; however, NAC treatment does not fully restore ovaries to wild-type size

decreased ovary weight ( J:242000 )

• females show a 77.4% reduction in ovary weight to body weight ratio at 8 weeks of age

• 3-week-old NAC-treated mice show a higher ovary weight to body weight ratio than PBS-treated mice

female infertility ( J:242000 )

• 8- to 10-week-old females fail to produce offspring during a 6-month mating period with wild-type males

cellular

abnormal oogenesis ( J:242000 )

• pregnant mare serum gonadotropin (PMSG)-primed females show abnormal oogenesis at 8 weeks of age

abnormal oocyte morphology ( J:242000 )

• at 3 weeks of age, the ratio of oocytes with >5 gamma-H2AX foci is ~56.8% versus ~14.6% in wild-type oocytes, indicating severe DNA damage (double-strand breaks)

• NAC-treated oocytes show a 58.7% reduction in double-strand breaks relative to PBS-treated oocytes

decreased oocyte number ( J:242000 )

• after PMSG priming, only ~20 full-grown germinal vesicle (GV) stage oocytes are recovered per female versus ~50 oocytes per wild-type control

• following superovulation with PMSG and HCG, more metaphase II (MII) oocytes are recovered from NAC-treated mice than from PBS-treated mice at 3 weeks of age

abnormal epigenetic regulation of gene expression ( J:242000 )

• 2-week-old females exhibit decreased H4K16 acetylation levels in oocytes

• however, acetylation at other detected histone residues is normal in oocytes

abnormal female germ cell physiology ( J:242000 )

• oocytes from 2-week-old females show decreased antioxidant gene expression and increased reactive oxygen species (ROS) levels

• following daily i.p. injection of antioxidant N-acetylcysteine (NAC) for 7 days, NAC-treated oocytes show a 67.1% reduction in ROS levels relative to PBS-treated oocytes

increased female germ cell apoptosis ( J:242000 )

• TUNEL assays revealed significant oocyte apoptosis at 3 weeks of age (~17.2% versus ~5.7% in wild-type oocytes)

• following daily i.p. injection of antioxidant N-acetylcysteine (NAC) for 7 days, NAC-treated oocytes show a 56.1% reduction in TUNEL+ cells

oxidative stress ( J:242000 )

• oocytes from 2-week-old females show significant downregulation of antioxidant genes (e.g. Prdx1, Prdx2, Gpx1, Gpx4) and a >2-fold increase in ROS levels relative to wild-type oocytes

• NAC-treated oocytes show a 67.1% reduction in ROS levels relative to PBS-treated oocytes

endocrine/exocrine glands

increased atretic ovarian follicle number ( J:242000 )

• ovaries contain 2-fold more atretic follicles at 3 weeks of age

• NAC-treated ovaries show a 54.7% reduction in the number of atretic follicles relative to PBS-treated ovaries

impaired ovarian folliculogenesis ( J:242000 )

• ovaries contain 50.4%, 49.6% and 78.7% fewer secondary, preantral and antral follicles at 3 weeks of age

• ovarian follicle development arrests in the secondary and preantral follicle stage; however, granulosa cell proliferation is normal in secondary and preantral follicles at 2 weeks of age

small ovary ( J:242000 )

• ovaries are markedly smaller at 8 weeks of age

decreased ovary weight ( J:242000 )

• females show a 77.4% reduction in ovary weight to body weight ratio at 8 weeks of age

• 3-week-old NAC-treated mice show a higher ovary weight to body weight ratio than PBS-treated mice

Allelic Composition	Csnk2b^tm1.1Bb/Csnk2b^tm1.1Bb Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129S2/SvPasCrl * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Csnk2b^tm1.1Bb mutation (0 available); any Csnk2b mutation (14 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased oocyte number ( J:305036 )

increased granulosa cell apoptosis ( J:305036 )

• TUNEL analysis revealed increased granulosa cell apoptosis at 4 weeks of age

absent primary ovarian follicles ( J:305036 )

• primary ovarian follicles are depleted at 8 weeks of age, with only a few MVH-positive primary follicles found scattered in the cortical region

• however, number of primary follicles per ovary is normal at 3, 4 and 6 weeks of age

decreased primordial ovarian follicle number ( J:305036 )

• number of primordial ovarian follicles per ovary is markedly reduced at 3, 4 and 6 weeks of age

absent primordial ovarian follicles ( J:305036 )

• primordial ovarian follicles become depleted by 8 weeks of age, with only a few MVH-positive primordial follicles found scattered in the cortical region

decreased secondary ovarian follicle number ( J:305036 )

• number of secondary follicles per ovary is markedly reduced at 6 weeks of age

absent secondary ovarian follicles ( J:305036 )

• secondary ovarian follicles become depleted by 8 weeks of age

decreased tertiary ovarian follicle number ( J:305036 )

• number of antral follicles per ovary is markedly reduced at 6 weeks of age

absent tertiary ovarian follicles ( J:305036 )

• antral ovarian follicles become depleted by 8 weeks of age

increased atretic ovarian follicle number ( J:305036 )

• although only a few follicles undergo atresia at 3 weeks, most follicles show signs of atresia by 4 weeks of age

• massive atretic follicles are noted at 6 weeks of age (sexual maturity): oocytes in atretic follicles are eliminated and the space is filled with granulosa cells

• nearly all types of follicles become depleted and no follicular structures are discernible in the ovaries by 8 weeks of age

decreased ovary weight ( J:305036 )

• ovary weight to body weight ratio is significantly decreased at 4, 6, and 8 weeks of age

ovary atrophy ( J:305036 )

• ovary size is only slightly smaller at 3 weeks but sharply decreased from 4 to 8 weeks of age

• by 8 weeks, ovary size is reduced to about one fourth of that in control mice, indicating ovarian atrophy

premature ovarian failure ( J:305036 )

• female infertility is attributed to premature ovarian failure caused by massive follicle atresia

female infertility ( J:305036 )

• 6-8-week-old female mice fail to produce offspring during a 6-month mating period with wild-type males of known fertility

cellular

decreased oocyte number ( J:305036 )

increased granulosa cell apoptosis ( J:305036 )

• TUNEL analysis revealed increased granulosa cell apoptosis at 4 weeks of age

abnormal double-strand DNA break repair ( J:305036 )

• at 2 weeks of age, small oocytes exhibit enhanced gammaH2AX signals indicative of accumulated unrepaired DSBs

• by 4 weeks of age, the DNA damage response pathway is elevated, as indicated by upregulation of phosphorylated p53 (S15) in the ovaries

mortality/aging

premature ovarian failure ( J:305036 )

• female infertility is attributed to premature ovarian failure caused by massive follicle atresia

endocrine/exocrine glands

increased granulosa cell apoptosis ( J:305036 )

• TUNEL analysis revealed increased granulosa cell apoptosis at 4 weeks of age

absent primary ovarian follicles ( J:305036 )

• primary ovarian follicles are depleted at 8 weeks of age, with only a few MVH-positive primary follicles found scattered in the cortical region

• however, number of primary follicles per ovary is normal at 3, 4 and 6 weeks of age

decreased primordial ovarian follicle number ( J:305036 )

• number of primordial ovarian follicles per ovary is markedly reduced at 3, 4 and 6 weeks of age

absent primordial ovarian follicles ( J:305036 )

• primordial ovarian follicles become depleted by 8 weeks of age, with only a few MVH-positive primordial follicles found scattered in the cortical region

decreased secondary ovarian follicle number ( J:305036 )

• number of secondary follicles per ovary is markedly reduced at 6 weeks of age

absent secondary ovarian follicles ( J:305036 )

• secondary ovarian follicles become depleted by 8 weeks of age

decreased tertiary ovarian follicle number ( J:305036 )

• number of antral follicles per ovary is markedly reduced at 6 weeks of age

absent tertiary ovarian follicles ( J:305036 )

• antral ovarian follicles become depleted by 8 weeks of age

increased atretic ovarian follicle number ( J:305036 )

• although only a few follicles undergo atresia at 3 weeks, most follicles show signs of atresia by 4 weeks of age

• massive atretic follicles are noted at 6 weeks of age (sexual maturity): oocytes in atretic follicles are eliminated and the space is filled with granulosa cells

• nearly all types of follicles become depleted and no follicular structures are discernible in the ovaries by 8 weeks of age

decreased ovary weight ( J:305036 )

• ovary weight to body weight ratio is significantly decreased at 4, 6, and 8 weeks of age

ovary atrophy ( J:305036 )

• ovary size is only slightly smaller at 3 weeks but sharply decreased from 4 to 8 weeks of age

• by 8 weeks, ovary size is reduced to about one fourth of that in control mice, indicating ovarian atrophy

premature ovarian failure ( J:305036 )

• female infertility is attributed to premature ovarian failure caused by massive follicle atresia

homeostasis/metabolism

abnormal double-strand DNA break repair ( J:305036 )

• at 2 weeks of age, small oocytes exhibit enhanced gammaH2AX signals indicative of accumulated unrepaired DSBs

• by 4 weeks of age, the DNA damage response pathway is elevated, as indicated by upregulation of phosphorylated p53 (S15) in the ovaries

Allelic Composition	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pten^tm1Hwu mutation (16 available); any Pten mutation (88 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

endocrine/exocrine glands

abnormal primordial ovarian follicle morphology ( J:151696 )

• females exhibit prematurely activated primordial follicles (transient follicles with enlarged oocytes surrounded by flattened pre-granulosa cells) leading to complete depletion of follicles by 16 weeks of age

reproductive system

abnormal primordial ovarian follicle morphology ( J:151696 )

Allelic Composition	Pten^tm1Hwu/Pten^tm1Hwu Tsc1^tm1Djk/Tsc1^tm1Djk Tg(Gdf9-icre)5092Coo/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pten^tm1Hwu mutation (16 available); any Pten mutation (88 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)
Tsc1^tm1Djk mutation (2 available); any Tsc1 mutation (71 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

endocrine/exocrine glands

abnormal ovarian follicle morphology ( J:155357 )

• mutants exhibit premature activation of the entire primordial follicle pool with an enhanced rate of oocyte growth and follicular development greater than in either single mutant

reproductive system

abnormal ovarian follicle morphology ( J:155357 )

• mutants exhibit premature activation of the entire primordial follicle pool with an enhanced rate of oocyte growth and follicular development greater than in either single mutant

Allelic Composition	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gdf9-icre)5092Coo/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pten^tm1Hwu mutation (16 available); any Pten mutation (88 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

endocrine/exocrine glands

abnormal primordial ovarian follicle morphology ( J:155357 )

• mutant females exhibit accelerated activation of primordial follicles

reproductive system

abnormal primordial ovarian follicle morphology ( J:155357 )

• mutant females exhibit accelerated activation of primordial follicles

Allelic Composition	Tsc1^tm1Djk/Tsc1^tm1Djk Tg(Gdf9-icre)5092Coo/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Gdf9-icre)5092Coo mutation (1 available)
Tsc1^tm1Djk mutation (2 available); any Tsc1 mutation (71 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

endocrine/exocrine glands

abnormal ovarian follicle morphology ( J:155357 )

• mutants exhibit premature activation of the entire primordial follicle pool by P23, leading to follicle depletion and premature ovarian failure in early adulthood (2-3 months of age)

• injection with rapamycin reverses the overactivation of primordial follicles, indicative that mTORC1 activity is responsible for increased activation of follicles

premature ovarian failure ( J:155357 )

• premature ovarian failure in early adulthood (2-3 months of age)

mortality/aging

premature ovarian failure ( J:155357 )

• premature ovarian failure in early adulthood (2-3 months of age)

homeostasis/metabolism

increased circulating follicle stimulating hormone level ( J:155357 )

• higher levels at 3-4 months of age

increased circulating luteinizing hormone level ( J:155357 )

• higher levels at 3-4 months of age

reproductive system

abnormal ovarian follicle morphology ( J:155357 )

• mutants exhibit premature activation of the entire primordial follicle pool by P23, leading to follicle depletion and premature ovarian failure in early adulthood (2-3 months of age)

• injection with rapamycin reverses the overactivation of primordial follicles, indicative that mTORC1 activity is responsible for increased activation of follicles

premature ovarian failure ( J:155357 )

• premature ovarian failure in early adulthood (2-3 months of age)

reduced female fertility ( J:155357 )

• from 6-27 weeks of age, females produce at most 2 litters of normal size and then become infertile after around 12-13 weeks

Allelic Composition	Pdpk1^tm1Maka/Pdpk1^tm1Maka Tsc1^tm1Djk/Tsc1^tm1Djk Tg(Gdf9-icre)5092Coo/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pdpk1^tm1Maka mutation (0 available); any Pdpk1 mutation (138 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)
Tsc1^tm1Djk mutation (2 available); any Tsc1 mutation (71 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

endocrine/exocrine glands

abnormal ovarian follicle morphology ( J:155357 )

• although some primordial follicles are activated by P23, not all of them are activated as seen in single conditional homozygous Tsc1 mutants, indicating partial reversal of the overactivation of primordial follicles

reproductive system

abnormal ovarian follicle morphology ( J:155357 )

Allelic Composition	Smc1b^tm1Jess/Smc1b^tm2.1Jess Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: 129S6/SvEvTac * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smc1b^tm1Jess mutation (0 available); any Smc1b mutation (69 available)
Smc1b^tm2.1Jess mutation (0 available); any Smc1b mutation (69 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:166776 )

N	• female mice are fertile • aged mice exhibit normal maintenance of sister chromatid cohesion and chiasma position

Allelic Composition	Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/Gt(ROSA)26Sor^{tm1(HBEGF)Awai} Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1(HBEGF)Awai} mutation (4 available); any Gt(ROSA)26Sor mutation (993 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal female germ cell apoptosis ( J:157008 )

• oocytes have undergone apoptosis in preantral follicles

abnormal granulosa cell apoptosis ( J:157008 )

• granulosa cells have undergone apoptosis in preantral follicles

reproductive system

abnormal female germ cell apoptosis ( J:157008 )

• oocytes have undergone apoptosis in preantral follicles

abnormal granulosa cell apoptosis ( J:157008 )

• granulosa cells have undergone apoptosis in preantral follicles

abnormal ovary morphology ( J:157008 )

abnormal ovarian follicle morphology ( J:157008 )

• with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis

endocrine/exocrine glands

abnormal granulosa cell apoptosis ( J:157008 )

• granulosa cells have undergone apoptosis in preantral follicles

abnormal ovary morphology ( J:157008 )

abnormal ovarian follicle morphology ( J:157008 )

Allelic Composition	Dcaf13^em2Hyfn/Dcaf13^em2Hyfn Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dcaf13^em2Hyfn mutation (0 available); any Dcaf13 mutation (42 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal oocyte morphology ( J:302700 )

• best grown oocytes from 3-week-old females are smaller in diameter, contain a germinal vesicle (GV) with unclear boundaries and aggregate dark cytoplasmic granules

• oocyte transition from the nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) type is inhibited; oocyte development is arrested in the NSN chromatin configuration, indicating impaired nucleolar maturation

absent oocytes ( J:302700 )

• ovaries show complete oocyte loss at 5 months of age

absent corpus luteum ( J:302700 )

• at 8 weeks of age, ovaries are devoid of corpora lutea

abnormal ovarian follicle morphology ( J:302700 )

• at 3 weeks of age, ovaries contain fewer growing follicles than those in control females

increased primary ovarian follicle number ( J:302700 )

• at 3 and 8 weeks of age, ovaries contain higher numbers of primary follicles than control ovaries

decreased primordial ovarian follicle number ( J:302700 )

• at 8 weeks of age, ovaries contain fewer primordial follicles than control ovaries

decreased secondary ovarian follicle number ( J:302700 )

• at 3 weeks of age, the number of secondary follicles with multilayer granulosa cells is significantly lower than in control ovaries

• however, the number of bilayer secondary follicles is similar to that in control ovaries

absent secondary ovarian follicles ( J:302700 )

• at 8 weeks of age, ovaries are devoid of follicles containing more than two layers of granulosa cells

absent ovarian follicles ( J:302700 )

• by 5 months of age, no ovarian follicles are detected

impaired ovarian folliculogenesis ( J:302700 )

• 8-week-old females lack follicles beyond the secondary follicle stage

• by 5 months of age, ovaries show complete oocyte and follicle loss

• in culture, secondary follicles containing two layers of granulosa cells from P21 females are arrested at the secondary stage and subsequently degenerate without developing to the antral stage

small ovary ( J:302700 )

• at 3 and 8 weeks of age, ovaries are smaller than in control females

premature ovarian failure ( J:302700 )

• complete oocyte and follicle loss results in premature ovarian failure at 5 months of age

abnormal superovulation ( J:302700 )

• 3-week-old females are unresponsive to PMSG treatment

failure of superovulation ( J:302700 )

• 3-week-old females fail to ovulate after PMSG plus hCG treatment

female infertility ( J:302700 )

• when 6-week-old females were mated with wild-type males for 5 months, no pups were born

endocrine/exocrine glands

absent corpus luteum ( J:302700 )

• at 8 weeks of age, ovaries are devoid of corpora lutea

abnormal ovarian follicle morphology ( J:302700 )

• at 3 weeks of age, ovaries contain fewer growing follicles than those in control females

increased primary ovarian follicle number ( J:302700 )

• at 3 and 8 weeks of age, ovaries contain higher numbers of primary follicles than control ovaries

decreased primordial ovarian follicle number ( J:302700 )

• at 8 weeks of age, ovaries contain fewer primordial follicles than control ovaries

decreased secondary ovarian follicle number ( J:302700 )

• at 3 weeks of age, the number of secondary follicles with multilayer granulosa cells is significantly lower than in control ovaries

• however, the number of bilayer secondary follicles is similar to that in control ovaries

absent secondary ovarian follicles ( J:302700 )

• at 8 weeks of age, ovaries are devoid of follicles containing more than two layers of granulosa cells

absent ovarian follicles ( J:302700 )

• by 5 months of age, no ovarian follicles are detected

impaired ovarian folliculogenesis ( J:302700 )

• 8-week-old females lack follicles beyond the secondary follicle stage

• by 5 months of age, ovaries show complete oocyte and follicle loss

• in culture, secondary follicles containing two layers of granulosa cells from P21 females are arrested at the secondary stage and subsequently degenerate without developing to the antral stage

small ovary ( J:302700 )

• at 3 and 8 weeks of age, ovaries are smaller than in control females

premature ovarian failure ( J:302700 )

• complete oocyte and follicle loss results in premature ovarian failure at 5 months of age

cellular

abnormal cell physiology ( J:302700 )

• at 3 weeks of age, the % of EU-positive oocytes is significantly higher than in control females, consistent with impaired NSN-SN transition; however, the intensity of EU signals in NSN oocytes is comparable to that in control oocytes, suggesting that global transcription activity is unaffected

• further analysis showed that growing oocytes show impaired 18S rRNA processing activity leading to 28S rRNA accumulation

abnormal oocyte morphology ( J:302700 )

• best grown oocytes from 3-week-old females are smaller in diameter, contain a germinal vesicle (GV) with unclear boundaries and aggregate dark cytoplasmic granules

absent oocytes ( J:302700 )

• ovaries show complete oocyte loss at 5 months of age

abnormal translation ( J:302700 )

• fluorescent staining with L-homopropargylglycine (HPG, an analog of methionine) showed reduced protein synthesis activity in both growing NSN-type and fully-grown SN-type oocytes isolated from 3-week-old females relative to control oocytes

homeostasis/metabolism

abnormal translation ( J:302700 )

mortality/aging

premature ovarian failure ( J:302700 )

• complete oocyte and follicle loss results in premature ovarian failure at 5 months of age

Allelic Composition	Rps26^tm1.1Jzc/Rps26^tm1.1Jzc Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6 * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rps26^tm1.1Jzc mutation (0 available); any Rps26 mutation (19 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature ovarian failure ( J:278629 )

• mice show retarded follicle development from pre-antral follicles to antral follicles, while the chromatin configurations of the oocytes are arrested at the transition from the non-surrounded nucleolus to surrounded nucleolus-type and all oocytes die by P84 resulting in premature ovarian failure

reproductive system

abnormal oocyte morphology ( J:278629 )

• germinal vesicle oocytes from P21 mice are smaller

• following 12 hours of in vitro culture, about 90% of oocytes are arrested at the germinal vesicle stage instead of extruding the first polar body

• 90% of oocytes are non-surrounded nucleolus (NSN)-type instead of surrounded nucleolus (SN)-type at P21, suggesting a failure of the NSN to SN transition; these NSN-type oocytes show a lack of histone and DNA methylation and are unable to complete meiosis process

absent oocytes ( J:278629 )

• no oocytes are present by P84

abnormal ovarian follicle number ( J:278629 )

• total numbers of follicles in ovaries are increased at P11, but gradually decline from P14 to P84 and the numbers of follicles at P28, P42, and P84 are lower

• at P42, the numbers of follicles, including the pre-antral and antral follicles, are reduced due to follicle atresia and only a few follicles develop into antral follicles and form corporal lutea after ovulation

abnormal ovarian folliculogenesis ( J:278629 )

• P11 ovaries contain more primordial follicles and fewer secondary follicles, indicating that most follicles are arrested at the primary follicle stage

• at P14, no antral follicles are seen in ovaries, although more pre-antral follicles are seen

• at P21, the number of primary and secondary follicles is increased, while few antral follicles are seen

• at P28, most follicles are still arrested at the pre-antral follicular stage, although a few follicles develop into antral follicles

• ovary shows poor response to treatment with pregnant mare serum gonadotropin (PMSG), indicating that follicles are mostly arrested in the pre-antral stage

small ovary ( J:278629 )

• ovaries are smaller from P14 to P84, but are normal sized at P11

premature ovarian failure ( J:278629 )

female infertility ( J:278629 )

endocrine/exocrine glands

abnormal ovarian follicle number ( J:278629 )

• total numbers of follicles in ovaries are increased at P11, but gradually decline from P14 to P84 and the numbers of follicles at P28, P42, and P84 are lower

abnormal ovarian folliculogenesis ( J:278629 )

• P11 ovaries contain more primordial follicles and fewer secondary follicles, indicating that most follicles are arrested at the primary follicle stage

• at P14, no antral follicles are seen in ovaries, although more pre-antral follicles are seen

• at P21, the number of primary and secondary follicles is increased, while few antral follicles are seen

• at P28, most follicles are still arrested at the pre-antral follicular stage, although a few follicles develop into antral follicles

• ovary shows poor response to treatment with pregnant mare serum gonadotropin (PMSG), indicating that follicles are mostly arrested in the pre-antral stage

small ovary ( J:278629 )

• ovaries are smaller from P14 to P84, but are normal sized at P11

premature ovarian failure ( J:278629 )

cellular

abnormal oocyte morphology ( J:278629 )

• germinal vesicle oocytes from P21 mice are smaller

• following 12 hours of in vitro culture, about 90% of oocytes are arrested at the germinal vesicle stage instead of extruding the first polar body

absent oocytes ( J:278629 )

• no oocytes are present by P84

Allelic Composition	Chd7^{tm2c(EUCOMM)Wtsi}/Chd7^{tm2c(EUCOMM)Wtsi} Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6 * C57BL/6J * C57BL/6N
Cell Lines	EPD0019_1_D07

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd7^{tm2c(EUCOMM)Wtsi} mutation (0 available); any Chd7 mutation (138 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

increased granulosa cell apoptosis ( J:324165 )

• a TUNEL assay showed that the rate of granulosa cell apoptosis in adult ovaries is significantly higher than in heterozygous control ovaries, esp. in the preantral and early antral follicles

• IHC showed that the expression of cleaved caspase-3 positive cells is significantly higher than in control females

• however, granulosa cell proliferation is normal, as determined by Ki-67 staining

decreased primary ovarian follicle number ( J:324165 )

decreased primordial ovarian follicle number ( J:324165 )

decreased secondary ovarian follicle number ( J:324165 )

decreased tertiary ovarian follicle number ( J:324165 )

increased atretic ovarian follicle number ( J:324165 )

• the percentage of atretic follicles is significantly greater than in control females

impaired ovarian folliculogenesis ( J:324165 )

• H&E staining showed a significantly decreased number of ovarian follicles at all stages of folliculogenesis

small ovary ( J:324165 )

• at 8 weeks of age, ovaries are significantly smaller than in wild-type females

• however, body size is normal

decreased ovary weight ( J:324165 )

• at 8 weeks of age, ovary weights are significantly lower than in wild-type females

• however, body weight is normal

reduced female fertility ( J:324165 )

• when mated with wild-type males for 6 months, females produce a significantly lower number of litters than wild-type or heterozygous controls

endocrine/exocrine glands

increased granulosa cell apoptosis ( J:324165 )

• a TUNEL assay showed that the rate of granulosa cell apoptosis in adult ovaries is significantly higher than in heterozygous control ovaries, esp. in the preantral and early antral follicles

• IHC showed that the expression of cleaved caspase-3 positive cells is significantly higher than in control females

• however, granulosa cell proliferation is normal, as determined by Ki-67 staining

decreased primary ovarian follicle number ( J:324165 )

decreased primordial ovarian follicle number ( J:324165 )

decreased secondary ovarian follicle number ( J:324165 )

decreased tertiary ovarian follicle number ( J:324165 )

increased atretic ovarian follicle number ( J:324165 )

• the percentage of atretic follicles is significantly greater than in control females

impaired ovarian folliculogenesis ( J:324165 )

• H&E staining showed a significantly decreased number of ovarian follicles at all stages of folliculogenesis

small ovary ( J:324165 )

• at 8 weeks of age, ovaries are significantly smaller than in wild-type females

• however, body size is normal

decreased ovary weight ( J:324165 )

• at 8 weeks of age, ovary weights are significantly lower than in wild-type females

• however, body weight is normal

cellular

increased granulosa cell apoptosis ( J:324165 )

• a TUNEL assay showed that the rate of granulosa cell apoptosis in adult ovaries is significantly higher than in heterozygous control ovaries, esp. in the preantral and early antral follicles

• IHC showed that the expression of cleaved caspase-3 positive cells is significantly higher than in control females

• however, granulosa cell proliferation is normal, as determined by Ki-67 staining

Allelic Composition	Aurka^{tm1c(EUCOMM)Hmgu}/Aurka^{tm1c(EUCOMM)Hmgu} Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6 * C57BL/6J * C57BL/6N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aurka^{tm1c(EUCOMM)Hmgu} mutation (0 available); any Aurka mutation (40 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal female meiosis I arrest ( J:307094 )

• after induction of ovulation with PMSG plus hCG, none of the cells harvested from the oviducts have polar bodies, and all cells are arrested in Metaphase I (Met I) indicating a failure to complete meiosis I (MI)

• in vitro, prophase I-arrested oocytes fail to undergo polar body extrusion after maturation; however, the % of oocytes that resume meiosis and undergo nuclear envelope breakdown in vitro is normal

• exogenous AURKA-EGFP expression rescues nearly all oocytes, restoring their ability to extrude polar bodies and reach Metaphase of meiosis II (Met II); in contrast, ectopic expression of AURKB-EGFP or AURKC-EYFP fails to rescue MI failure and none of these oocytes extrude a polar body

abnormal meiotic spindle morphology ( J:307094 )

• in vitro, oocytes exhibit a delay in resolving individual chromosomes during meiotic maturation and show defects in MI spindle building, with inability to fragment aMTOCs (acentriolar multiple microtubule-organizing centers) upon exiting from prophase I and loss of localized TACC3 protein (a known AURKA substrate required for spindle building)

• 55% of oocytes show monopolar spindles while ~45% have short bipolar spindles with significantly reduced spindle length and volume at Met I

• exogenous expression of AURKA rescues MI spindle volume and restores chromosome resolution to wild-type like kinetics, resulting in stable, bipolar MI spindle formation; in contrast, expression of AURKB-EGFP fails to rescue all of these parameters whereas AURKC-EYFP partially rescues the time to spindle bipolarization

decreased corpora lutea number ( J:307094 )

• at 2 months of age, ovaries contain about half the number of corpora lutea (CL) observed in wild-type ovaries

• however, follicle development and ovulation are normal, and no statistically significant reduction in CL number seen at 6 months of age

female infertility ( J:307094 )

• sexually mature females mated with age-matched wild-type male mice of proven fertility never produced a pup during a 4-month fertility trial

endocrine/exocrine glands

decreased corpora lutea number ( J:307094 )

• at 2 months of age, ovaries contain about half the number of corpora lutea (CL) observed in wild-type ovaries

• however, follicle development and ovulation are normal, and no statistically significant reduction in CL number seen at 6 months of age

cellular

abnormal female meiosis I arrest ( J:307094 )

abnormal meiotic spindle morphology ( J:307094 )

• 55% of oocytes show monopolar spindles while ~45% have short bipolar spindles with significantly reduced spindle length and volume at Met I

Allelic Composition	Rtcb^tm1Hyfn/Rtcb^tm1Hyfn Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rtcb^tm1Hyfn mutation (0 available); any Rtcb mutation (25 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased secondary ovarian follicle number ( J:330334 )

• Fewer secondary follicles are found at 6 weeks of age.

decreased tertiary ovarian follicle number ( J:330334 )

• Fewer antral follicles are found at 6 weeks of age.

absent ovarian follicles ( J:330334 )

• No ovarian follicles are found at 10 weeks or 7 months of age.

small ovary ( J:330334 )

• At 4 weeks of age, ovary size is comparable to controls. By 6 weeks of age and thereafter, ovary size is significantly smaller than controls.

female infertility ( J:330334 )

endocrine/exocrine glands

decreased secondary ovarian follicle number ( J:330334 )

• Fewer secondary follicles are found at 6 weeks of age.

decreased tertiary ovarian follicle number ( J:330334 )

• Fewer antral follicles are found at 6 weeks of age.

absent ovarian follicles ( J:330334 )

• No ovarian follicles are found at 10 weeks or 7 months of age.

small ovary ( J:330334 )

• At 4 weeks of age, ovary size is comparable to controls. By 6 weeks of age and thereafter, ovary size is significantly smaller than controls.

Allelic Composition	Smc1b^tm2.1Jess/Smc1b^tm2.1Jess Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smc1b^tm2.1Jess mutation (0 available); any Smc1b mutation (69 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:166776 )

N	• female mice are fertile • aged mice exhibit normal maintenance of sister chromatid cohesion and chiasma position

Allelic Composition	Foxl2^tm2.1Tre/Foxl2^tm2.1Tre Tg(Gdf9-icre)5092Coo/0
Genetic Background	involves: C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxl2^tm2.1Tre mutation (0 available); any Foxl2 mutation (15 available)
Tg(Gdf9-icre)5092Coo mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:157008 )

N	• mice have no fertility defects

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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