Phenotypes associated with this allele

• Allele Symbol: Tg(H2KmPCC)2939Stoe
• Allele Name: transgene insertion 2939, Stephan Oehen
• Allele ID: MGI:3056682
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(H2KmPCC)2939Stoe/0 Tg(Tcra2B4)1Mmd/0 Tg(Tcrb2B4)1Mmd/0	involves: C3H/HeJ * C57BL/6 * SJL	MGI:3688409
cx2	Tg(H2KmPCC)2939Stoe/? Tg(TcraTcrbAD10)1Hed/?	involves: C57BL/6 * SJL	MGI:3835454
cx3	Tg(H2KmPCC)2939Stoe/? Tg(TcrAND)53Hed/?	involves: C57BL/6 * SJL	MGI:3835458
tg4	Tg(H2KmPCC)2939Stoe/?	involves: C57BL/6 * SJL	MGI:3835453

Genotype
MGI:3688409

cx1

• Allelic Composition: Tg(H2KmPCC)2939Stoe/0; Tg(Tcra2B4)1Mmd/0; Tg(Tcrb2B4)1Mmd/0
• Genetic Background: involves: C3H/HeJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:50143 )

• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response

decreased thymocyte number ( J:50143 )

• number of thymocytes (2-4 x 10⁶) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 10⁶ cells)

abnormal T cell differentiation ( J:50143 )

• number of thymocytes is drastically reduced and few thymocytes undergo natural development using the endogenous Tcra/transgenic Tcrb chain compared to Tcra-knockin, transgenic Tcrb-expressing mice

hematopoietic system

decreased T cell proliferation ( J:50143 )

• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response

decreased thymocyte number ( J:50143 )

• number of thymocytes (2-4 x 10⁶) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 10⁶ cells)

abnormal T cell differentiation ( J:50143 )

• number of thymocytes is drastically reduced and few thymocytes undergo natural development using the endogenous Tcra/transgenic Tcrb chain compared to Tcra-knockin, transgenic Tcrb-expressing mice

endocrine/exocrine glands

decreased thymocyte number ( J:50143 )

• number of thymocytes (2-4 x 10⁶) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 10⁶ cells)

cellular

decreased T cell proliferation ( J:50143 )

• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response

Genotype
MGI:3835454

cx2

• Allelic Composition: Tg(H2KmPCC)2939Stoe/?; Tg(TcraTcrbAD10)1Hed/?
• Genetic Background: involves: C57BL/6 * SJL

immune system

decreased CD4-positive, alpha-beta T cell number ( J:92997 )

• the number of CD4 single-positive thymocytes are severely reduced in these mice

• peripheral CD4⁺ T cells are reduced from 41% of lymph node cells to 14%

abnormal immune tolerance ( J:92997 )

• negative selection of transgenic T cells leads to diminished CD4 T cells in the periphery

• apoptotic cells resulting from negative selection are found at the cortico-medullary and medullary portions of the thymus

abnormal T cell anergy ( J:92997 )

• T cells that escape central tolerance are able to respond to pigeon cytochrome-c but 10 times as much protein is needed to get a comparable response to Tg(TcrAND)53Hed transgenic

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:92997 )

• the number of CD4 single-positive thymocytes are severely reduced in these mice

• peripheral CD4⁺ T cells are reduced from 41% of lymph node cells to 14%

Genotype
MGI:3835458

cx3

• Allelic Composition: Tg(H2KmPCC)2939Stoe/?; Tg(TcrAND)53Hed/?
• Genetic Background: involves: C57BL/6 * SJL

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:92997 )

• the number of CD4 single-positive thymocytes are severely reduced in these mice

• peripheral CD4⁺ T cells are reduced by 75%

immune system

decreased CD4-positive, alpha-beta T cell number ( J:92997 )

• the number of CD4 single-positive thymocytes are severely reduced in these mice

• peripheral CD4⁺ T cells are reduced by 75%

abnormal immune tolerance ( J:92997 )

• negative selection of transgenic T cells leads to diminished CD4 T cells in the periphery

• apoptotic cells resulting from negative selection are found at the cortico-medullary and medullary portions of the thymus

abnormal T cell anergy ( J:92997 )

• T cells that escape central tolerance are able to respond to pigeon cytochrome C but 10 times as much protein is needed to get a comparable response to Tg(TcrAND)53Hed transgenic

Genotype
MGI:3835453

tg4

• Allelic Composition: Tg(H2KmPCC)2939Stoe/?
• Genetic Background: involves: C57BL/6 * SJL

immune system

abnormal immune tolerance ( J:92997 )

• lymph node cells are tolerant to pigeon cytochrome-c and fail to respond when cultured in vitro with the protein