Phenotypes associated with this allele

• Allele Symbol: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}
• Allele Name: transgene insertion 57, Richard A Lang
• Allele ID: MGI:3057163
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Siglec1^tm1(HBEGF)Mtka/Siglec1^tm1(HBEGF)Mtka 1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/?	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:3721136
cx2	Tg(Ins2-TFRC/OVA)296Wehi/0 1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	involves: C57BL/6 * FVB	MGI:3784427
ot3	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	B6.FVB-1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan	MGI:5476621
ot4	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	B6.FVB-1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/J	MGI:3784423
ot5	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	C.FVB-1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan	MGI:3784404
ot6	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	C.FVB-1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/J	MGI:3784466
ot7	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	involves: C57BL/6 * FVB/N	MGI:3801089
ot8	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	involves: FVB/N	MGI:5444881
ot9	1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/0	NOD.FVB-1700016L21Rik^Tg(Itgax-HBEGF/EGFP)57Lan/Jdk	MGI:3844521

Genotype
MGI:3721136

cx1

• Allelic Composition: Siglec1^{tm1(HBEGF)Mtka}/Siglec1^{tm1(HBEGF)Mtka}; 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal metallophilic macrophage morphology ( J:123958 )

• following diptheria toxin (DT) treatment, metallophilic macrophages and marginal zone macrophages are absent

abnormal spleen marginal zone macrophage morphology ( J:123958 )

• following diptheria toxin (DT) treatment, metallophilic macrophages and marginal zone macrophages are absent

abnormal immune system physiology ( J:123958 )

abnormal macrophage physiology ( J:123958 )

• following diptheria toxin (DT) treatment, clearance of injected apoptotic cells injected into the spleen is delayed

abnormal dendritic cell physiology ( J:123958 )

• following diptheria toxin (DT) treatment, more Itgax+ dendritic cells phagocytose apoptotic cells than in wild-type mice

• following diptheria toxin (DT) treatment, the antigen presenting ability of Itgax+ dendritic cells is increased relative to in wild-type mice

abnormal immune tolerance ( J:123958 )

• following diptheria toxin (DT) treatment, induction of tolerance fails

hematopoietic system

abnormal metallophilic macrophage morphology ( J:123958 )

• following diptheria toxin (DT) treatment, metallophilic macrophages and marginal zone macrophages are absent

abnormal spleen marginal zone macrophage morphology ( J:123958 )

• following diptheria toxin (DT) treatment, metallophilic macrophages and marginal zone macrophages are absent

abnormal macrophage physiology ( J:123958 )

• following diptheria toxin (DT) treatment, clearance of injected apoptotic cells injected into the spleen is delayed

Genotype
MGI:3784427

cx2

• Allelic Composition: Tg(Ins2-TFRC/OVA)296Wehi/0; 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: involves: C57BL/6 * FVB

immune system

decreased T cell proliferation ( J:122114 )

• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG

• however, transfer of dendritic cells into depleted mice restores proliferation

hematopoietic system

decreased T cell proliferation ( J:122114 )

• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG

• however, transfer of dendritic cells into depleted mice restores proliferation

cellular

decreased T cell proliferation ( J:122114 )

• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG

• however, transfer of dendritic cells into depleted mice restores proliferation

Genotype
MGI:5476621

ot3

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: B6.FVB-1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}

immune system

decreased neutrophil cell number ( J:194800 )

• at 24 hours after diphtheria toxin injection, numbers of PMNs are significantly decreased in bone marrow compared to controls

increased neutrophil cell number ( J:194800 )

• 6 hours after diphtheria toxin injection to induce dendritic cell depletion in noninfected mice, slightly increased polymorphonuclear neutrophil (PMN) cell numbers are detected in the blood, with a 2-fold increase after 24 hours, and peaking at a 5-fold increase at 72 hours after DT treatment; higher intrarenal PMN numbers are seen at 24 and 72 hour

decreased dendritic cell number ( J:194800 )

• with injection of diphtheria toxin (DT) when transurethral instillation of uropathogenic E. coli (UPEC) is performed to produce a model of pyelonephritis, kidney dendritic cell numbers are reduced by 50% by 6 hours after DT treatment; 30 hours after DT-induced dendritic cell depletion, dendritic cell numbers are decreased by more than 90% in kidney

• the remaining dendritic cells are not functional, as they do not produce chemokines to attract polymorphonuclear neutrophils

increased monocyte cell number ( J:194800 )

• blood monocyte numbers are 2-3 fold greater than control values at 72 hours after DT injection; intrarenal macrophage numbers are increased, peaking at 72 hours after DT

impaired neutrophil recruitment ( J:194800 )

• with diphtheria toxin injection at time of induction of pyelonephritis by transurethral infection by UPEC, polymorphonuclear neutrophil (PMN) recruitment is decreased

abnormal circulating cytokine level ( J:194800 )

• after DT injection, an increase in Cxcl2 level is observed in serum, but not in Il1, 4, 5 or 7, Tnf, Ccl2, 3, or 5 levels

abnormal susceptibility to infection ( J:194800 )

• at 30 hours after dendritic cell depletion in kidney by DT injection and infection with uropathogenic E. coli, numbers of polymorphonuclear neutrophils in kidneys are elevated and a 5-fold decrease in colony forming units of UPEC is observed compared to nondepleted control animals

increased susceptibility to bacterial infection ( J:194800 )

• with diphtheria toxin at time of induction of pyelonephritis by transurethral infection by UPEC, animals show impaired bacterial clearance

hematopoietic system

decreased neutrophil cell number ( J:194800 )

• at 24 hours after diphtheria toxin injection, numbers of PMNs are significantly decreased in bone marrow compared to controls

increased neutrophil cell number ( J:194800 )

• 6 hours after diphtheria toxin injection to induce dendritic cell depletion in noninfected mice, slightly increased polymorphonuclear neutrophil (PMN) cell numbers are detected in the blood, with a 2-fold increase after 24 hours, and peaking at a 5-fold increase at 72 hours after DT treatment; higher intrarenal PMN numbers are seen at 24 and 72 hour

decreased dendritic cell number ( J:194800 )

• with injection of diphtheria toxin (DT) when transurethral instillation of uropathogenic E. coli (UPEC) is performed to produce a model of pyelonephritis, kidney dendritic cell numbers are reduced by 50% by 6 hours after DT treatment; 30 hours after DT-induced dendritic cell depletion, dendritic cell numbers are decreased by more than 90% in kidney

• the remaining dendritic cells are not functional, as they do not produce chemokines to attract polymorphonuclear neutrophils

increased monocyte cell number ( J:194800 )

• blood monocyte numbers are 2-3 fold greater than control values at 72 hours after DT injection; intrarenal macrophage numbers are increased, peaking at 72 hours after DT

impaired neutrophil recruitment ( J:194800 )

• with diphtheria toxin injection at time of induction of pyelonephritis by transurethral infection by UPEC, polymorphonuclear neutrophil (PMN) recruitment is decreased

homeostasis/metabolism

abnormal circulating cytokine level ( J:194800 )

• after DT injection, an increase in Cxcl2 level is observed in serum, but not in Il1, 4, 5 or 7, Tnf, Ccl2, 3, or 5 levels

Genotype
MGI:3784423

ot4

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: B6.FVB-1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/J

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:113232 )

• mice exhibit an increase in mortality compared to wild-type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)

• however, replacement of dendritic cells with wild-type dendritic cells improves survival (mortality drops from 100% to 65%)

immune system

immune system phenotype ( J:96123 , J:122114 )

N • conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin (J:96123)

N • depletion of Cd11c+ dendritic cells by treatment with diphtheria toxin does not affect accumulation and localization of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in T cell zones of the lymph node and spleen (J:122114)

decreased dendritic cell number ( J:96123 , J:100867 , J:113232 )

• 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells (J:96123)

• CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin (J:100867)

• treatment with diphtheria toxin depletes CD11c^highMHCII^high dendritic cells (J:113232)

abnormal NK cell physiology ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin

• following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin

impaired natural killer cell mediated cytotoxicity ( J:125611 )

• unlike in wild-type mice, NK T cell activation measured by specific cell lysis following infection with Leishmania infantum in diphtheria toxin treated mice does not occur

abnormal NK T cell physiology ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma and 8% for IL-4 compared to 32% and 30%, respectively, of similarly treated wild-type NK T cells

• however, normal levels of IFN-gamma and IL-4 production where observed in liver NK T cells

decreased circulating interferon-gamma level ( J:96123 )

• following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin

decreased circulating interleukin-12 level ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin

decreased interferon-gamma secretion ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma compared to 32% of similarly treated wild-type NK T cells

• however, normal levels of production where observed in liver NK T cells

• following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin

decreased interleukin-12b secretion ( J:125611 )

• unlike in wild-type mice, IL-12p40 production following infection with Leishmania infantum in diphtheria toxin treated mice does not occur

decreased interleukin-4 secretion ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 8% of spleen NK T cells stain positive for IL-4 compared to 30% of similarly treated wild-type NK T cells however, normal levels of production where observed in liver NK T cells

sepsis ( J:113232 )

• mice exhibit an increase in mortality compared to wild-type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)

homeostasis/metabolism

decreased circulating interferon-gamma level ( J:96123 )

• following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin

decreased circulating interleukin-12 level ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin

hematopoietic system

decreased dendritic cell number ( J:96123 , J:100867 , J:113232 )

• 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells (J:96123)

• CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin (J:100867)

• treatment with diphtheria toxin depletes CD11c^highMHCII^high dendritic cells (J:113232)

abnormal NK cell physiology ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin

• following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin

impaired natural killer cell mediated cytotoxicity ( J:125611 )

• unlike in wild-type mice, NK T cell activation measured by specific cell lysis following infection with Leishmania infantum in diphtheria toxin treated mice does not occur

abnormal NK T cell physiology ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma and 8% for IL-4 compared to 32% and 30%, respectively, of similarly treated wild-type NK T cells

• however, normal levels of IFN-gamma and IL-4 production where observed in liver NK T cells

Genotype
MGI:3784404

ot5

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: C.FVB-1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}

mortality/aging

premature death ( J:93488 )

• repeated treatment with diphtheria toxin results in lethality due to dendritic cell depletion

immune system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:93488 )

• adoptive transfer experiments demonstrate that CD8+ T cell priming with cell-associated antigen does not occur in diphtheria-induced dendritic cell depleted mice

increased neutrophil cell number ( J:194800 )

• neutrophilia is observed in blood after diphtheria toxin treatment, indicating that there is no background effect with respect to C57BL/6

decreased dendritic cell number ( J:93488 )

• CD11c+ (Itgax+) dendritic cells are depleted for 2 days following treatment with diphtheria toxin

decreased CD8-positive, alpha-beta T cell number ( J:93488 )

• treatment with diphtheria toxin results in a substantial loss of CD8+ T cells

decreased cytotoxic T cell cytolysis ( J:93488 )

• in adoptive transfer experiments using diphtheria-induced dendritic cell depleted mice infected with Listeria or malaria at the liver stage

abnormal T cell activation ( J:93488 )

• treatment with diphtheria toxin results in a loss of in vitro stimulation of alloreactive T cells

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:93488 )

• adoptive transfer experiments demonstrate that CD8+ T cell priming with cell-associated antigen does not occur in diphtheria-induced dendritic cell depleted mice

increased neutrophil cell number ( J:194800 )

• neutrophilia is observed in blood after diphtheria toxin treatment, indicating that there is no background effect with respect to C57BL/6

decreased dendritic cell number ( J:93488 )

• CD11c+ (Itgax+) dendritic cells are depleted for 2 days following treatment with diphtheria toxin

decreased CD8-positive, alpha-beta T cell number ( J:93488 )

• treatment with diphtheria toxin results in a substantial loss of CD8+ T cells

decreased cytotoxic T cell cytolysis ( J:93488 )

• in adoptive transfer experiments using diphtheria-induced dendritic cell depleted mice infected with Listeria or malaria at the liver stage

abnormal T cell activation ( J:93488 )

• treatment with diphtheria toxin results in a loss of in vitro stimulation of alloreactive T cells

Genotype
MGI:3784466

ot6

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: C.FVB-1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/J

immune system

immune system phenotype ( J:96123 )

N • conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin

decreased dendritic cell number ( J:96123 )

• 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells

abnormal NK cell physiology ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin

decreased circulating interferon-gamma level ( J:96123 )

• following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin

decreased circulating interleukin-12 level ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin

decreased interferon-gamma secretion ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin

homeostasis/metabolism

decreased circulating interferon-gamma level ( J:96123 )

• following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin

decreased circulating interleukin-12 level ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin

hematopoietic system

decreased dendritic cell number ( J:96123 )

• 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells

abnormal NK cell physiology ( J:96123 )

• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin

Genotype
MGI:3801089

ot7

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: involves: C57BL/6 * FVB/N

immune system

decreased dendritic cell number ( J:137452 )

• CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin

• mesenteric lymph nodes are partially depleted following intratracheally administration of diphtheria toxin

abnormal alveolar macrophage morphology ( J:137452 )

• CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin

decreased interferon-gamma secretion ( J:137452 )

• following administration of diphtheria toxin and CD3 stimulation, IFN-gamma production by CD4 and CD8 T cells from the mesenteric lymph nodes is decreased compared to in similarly treated wild-type mice

increased susceptibility to Orthomyxoviridae infection ( J:137452 )

• following intratracheally administration of diphtheria toxin, mice exhibit increased severity of influenza infection, increased weight loss and delayed clearance compared to infected wild-type mice

• the number of virus-specific cytotoxic T lymphoctes is reduced in mice treated with diphtheria toxin and infected with influenza compared to similarly treated wild-type mcie

respiratory system

abnormal alveolar macrophage morphology ( J:137452 )

• CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin

hematopoietic system

decreased dendritic cell number ( J:137452 )

• CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin

• mesenteric lymph nodes are partially depleted following intratracheally administration of diphtheria toxin

abnormal alveolar macrophage morphology ( J:137452 )

• CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin

Genotype
MGI:5444881

ot8

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: involves: FVB/N

immune system

decreased leukocyte cell number ( J:193548 )

• in the skin of diphtheria toxin- and IMQ-treated mice

decreased neutrophil cell number ( J:193548 )

• in the skin of diphtheria toxin- and IMQ-treated mice

decreased T cell number ( J:193548 )

• decreased alpha-beta T cells in the skin of diphtheria toxin- and IMQ-treated mice

decreased gamma-delta T cell number ( J:193548 )

• in the skin of diphtheria toxin- and IMQ-treated mice

decreased interleukin-17 secretion ( J:193548 )

• in diphtheria toxin- and IMQ-treated mice

abnormal response to infection ( J:189122 )

• despite normal monocyte numbers in the blood, LPS-treated mice fail to exhibit an accumulation of CD11c+MHCII+DC-SIGN/CD209+CD14+ cells in the skin lymph nodes unlike wild-type mice

decreased susceptibility to bacterial infection ( J:210086 )

• mice treated with antibiotic, then diphtheria toxin (DTR) and infected with non-invasive Salmonella exhibit decreased bacterial titers in the mesenteric lymph nodes as compared to DTR-untreated controls

hematopoietic system

decreased leukocyte cell number ( J:193548 )

• in the skin of diphtheria toxin- and IMQ-treated mice

decreased neutrophil cell number ( J:193548 )

• in the skin of diphtheria toxin- and IMQ-treated mice

decreased T cell number ( J:193548 )

• decreased alpha-beta T cells in the skin of diphtheria toxin- and IMQ-treated mice

decreased gamma-delta T cell number ( J:193548 )

• in the skin of diphtheria toxin- and IMQ-treated mice

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:193548 )

• mice treated with diphtheria toxin and IMQ are protected from psoriasis development with decreased ear swelling and numbers of leukocytes, neutrophils and gamma-delta and alpha-beta T cells in the skin compared with wild-type mice

Genotype
MGI:3844521

ot9

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: NOD.FVB-1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/Jdk

mortality/aging

premature death ( J:137009 )

• diphtheria toxin-treated mice produce a large spike of IFN-gamma and TNF-alpha before developing cachexia and dying unlike similarly treated wild-type mice

immune system

insulitis ( J:137009 )

• irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type myeloid dendritic cells and have been treated with diphtheria toxin develop insulitis

• however, use of plasmocytoid dendritic cells instead of myeloid dendritic cells fails to restore development of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells and treated with diphtheria toxin exhibit an acceleration of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type plasmocytoid dendritic cells and treated with diphtheria toxin exhibit delay in the development of insulitis

decreased dendritic cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit ablation of all major dendritic cells from the spleen, pancreas, and lymph nodes unlike similarly treated wild-type mice

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic plasmacytoid dendritic cells

decreased NK T cell number ( J:137009 )

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic NK T cells

• however, spleen and nondraining secondary lymphoid compartments exhibit normal NK T cell numbers and the addition of plasmocytoid dendritic cells to mice restores pancreatic NK T cell numbers

decreased macrophage cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit macrophage loss that is less than 5% to 8%

abnormal professional antigen presenting cell physiology ( J:137009 )

• antigen presenting cells from irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells have been treated with diphtheria toxin fail to present either peptide or whole islet cells to naive BDC2.5 T cells over a wide range of antigen concentrations unlike PBS-treated mice

• however, additional treatment with wild-type myeloid dendritic cells restores antigen presentation

increased interferon-gamma secretion ( J:137009 )

• in diphtheria toxin-treated mice

increased tumor necrosis factor secretion ( J:137009 )

• in diphtheria toxin-treated mice

decreased susceptibility to autoimmune diabetes ( J:137009 )

• irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells have been treated with diphtheria toxin do not develop diabetes unlike PBS-treated mice

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:137009 )

N • irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells have been treated with diphtheria toxin fail to develop insulitis unlike PBS-treated mice

insulitis ( J:137009 )

• irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type myeloid dendritic cells and have been treated with diphtheria toxin develop insulitis

• however, use of plasmocytoid dendritic cells instead of myeloid dendritic cells fails to restore development of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells and treated with diphtheria toxin exhibit an acceleration of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type plasmocytoid dendritic cells and treated with diphtheria toxin exhibit delay in the development of insulitis

growth/size/body

cachexia ( J:137009 )

• in diphtheria toxin-treated mice

hematopoietic system

decreased dendritic cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit ablation of all major dendritic cells from the spleen, pancreas, and lymph nodes unlike similarly treated wild-type mice

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic plasmacytoid dendritic cells

decreased NK T cell number ( J:137009 )

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic NK T cells

• however, spleen and nondraining secondary lymphoid compartments exhibit normal NK T cell numbers and the addition of plasmocytoid dendritic cells to mice restores pancreatic NK T cell numbers

decreased macrophage cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit macrophage loss that is less than 5% to 8%