Phenotypes associated with this allele

• Allele Symbol: Tg(IL3)1Ygy
• Allele Name: transgene insertion 1, Yong-guang Yang
• Allele ID: MGI:3057194
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(CSF2)2Ygy/0 Tg(IL3)1Ygy/0 Tg(KITLG)3Ygy/0	C.Cg-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy	MGI:3620464
cx2	Prkdc^scid/Prkdc^scid Tg(CSF2)2Ygy/0 Tg(IL3)1Ygy/0	CB17.Cg-Prkdc^scid Tg(CSF2)2Ygy Tg(IL3)1Ygy	MGI:3620239
cx3	Prkdc^scid/Prkdc^scid Tg(CSF2)2Ygy/0 Tg(IL3)1Ygy/0 Tg(KITLG)3Ygy/0	CB17.Cg-Prkdc^scid Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy	MGI:3620237
cx4	Prkdc^scid/Prkdc^scid Tg(CSF2)2Ygy/0 Tg(IL3)1Ygy/0 Tg(KITLG)3Ygy/0	NOD.Cg-Prkdc^scid Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy	MGI:3620462

Genotype
MGI:3620464

cx1

• Allelic Composition: Tg(CSF2)2Ygy/0; Tg(IL3)1Ygy/0; Tg(KITLG)3Ygy/0
• Genetic Background: C.Cg-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal response to transplant ( J:107052 )

• low levels of porcine chimerism are detected in the blood of recipent mice at 1 week, but all detectable chimerism in the blood is lost by 2 weeks;at 19-22 weeks after transplant, no chimerism is detected in the spleen or bone marrow

decreased susceptibility to graft versus host disease ( J:107052 )

• conditioned mice were injected with splenocytes, or PBMC and BMC from sensitized porcine donors, no recipients showed detectable GVHD

Genotype
MGI:3620239

cx2

• Allelic Composition: Prkdc^scid/Prkdc^scid; Tg(CSF2)2Ygy/0; Tg(IL3)1Ygy/0
• Genetic Background: CB17.Cg-Prkdc^scid Tg(CSF2)2Ygy Tg(IL3)1Ygy

immune system

abnormal response to transplant ( J:94302 )

• engraftment of porcine bone marrow cells after bone marrow transplant into irradiated transgenic SCID mice is achieved and porcine hematopoesis is maintained at 7 weeks post-transplant with less efficiency than in mice also expressing the KITLG transgene

Genotype
MGI:3620237

cx3

• Allelic Composition: Prkdc^scid/Prkdc^scid; Tg(CSF2)2Ygy/0; Tg(IL3)1Ygy/0; Tg(KITLG)3Ygy/0
• Genetic Background: CB17.Cg-Prkdc^scid Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy

immune system

abnormal response to transplant ( J:94302 , J:106416 )

• at 1 week after transplant of bone marrow cells or PBMC from swine donors in irradiated transgenic SCID recipient mice, the percentage of porcine cells in the WBC in medronate-liposome treated (for depletion of macrophages) recipients is 10x greater than in PBS treated controls (J:94302)

• chimerism in medronate-liposome treated mice at 8 weeks post-transplant is 60% in the marrow compared to less than 30% in PBS treated controls (J:94302)

• engraftment of porcine bone marrow cells after bone marrow transplant into irradiated transgenic SCID mice is achieved and porcine hematopoesis is maintained at 7 weeks post-transplant compared to non transgenic littermates which lose porcine chimerism completely (J:106416)

Genotype
MGI:3620462

cx4

• Allelic Composition: Prkdc^scid/Prkdc^scid; Tg(CSF2)2Ygy/0; Tg(IL3)1Ygy/0; Tg(KITLG)3Ygy/0
• Genetic Background: NOD.Cg-Prkdc^scid Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy

immune system

abnormal response to transplant ( J:107052 )

• blood of NOD-SCID mouse recipients remains chimeric for porcine hematopoietic cells throughout the length of the experiment (6 weeks):

decreased susceptibility to graft versus host disease ( J:107052 )

• conditioned mice were injected with splenocytes, or PBMC and BMC from sensitized porcine donors, no recipients showed detectable GVHD