Phenotypes associated with this allele

• Allele Symbol: Tg(Thy1-YFP)16Jrs
• Allele Name: transgene insertion 16, Joshua R Sanes
• Allele ID: MGI:3505585
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Nrp1^tm1Ddg/Nrp1^tm1Ddg Tg(Thy1-YFP)16Jrs/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3512124
cx2	Fgfbp1^tm1Gvdz/Fgfbp1^tm1Gvdz Tg(Thy1-YFP)16Jrs/0	involves: 129S5/SvEvBrd * C57BL/6J * CBA	MGI:5902844
cx3	Fgfbp1^tm1Gvdz/Fgfbp1^tm1Gvdz Tg(SOD1*G93A)1Gur/0 Tg(Thy1-YFP)16Jrs/0	involves: 129S5/SvEvBrd * C57BL/6J * CBA * SJL	MGI:5902841
cx4	Gars1^C201R/Gars1^+ Tg(Thy1-YFP)16Jrs/?	involves: BALB/cAnN * C3H/HeH * C57BL/6J * CBA	MGI:5752648
cx5	Dynlrb1^tm1a(EUCOMM)Wtsi/Dynlrb1^+ Tg(Thy1-YFP)16Jrs/0	involves: C57BL/6J * C57BL/6N	MGI:6466700
cx6	Gars1^Nmf249/Gars1^+ Tg(Thy1-YFP)16Jrs/?	involves: C57BL/6J * CAST/Ei * CBA/J	MGI:5752579

Genotype
MGI:3512124

cx1

• Allelic Composition: Nrp1^tm1Ddg/Nrp1^tm1Ddg; Tg(Thy1-YFP)16Jrs/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal cerebral cortex pyramidal cell morphology ( J:93799 )

• basal dendrites of layer 5 cortical neurons within the neocortex were markedly diminished in length and complexity

Genotype
MGI:5902844

cx2

• Allelic Composition: Fgfbp1^tm1Gvdz/Fgfbp1^tm1Gvdz; Tg(Thy1-YFP)16Jrs/0
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J * CBA

nervous system

abnormal neuromuscular synapse morphology ( J:237911 )

• increased number of fragmented neuromuscular junctions at 12 months compared with control mice

Genotype
MGI:5902841

cx3

• Allelic Composition: Fgfbp1^tm1Gvdz/Fgfbp1^tm1Gvdz; Tg(SOD1*G93A)1Gur/0; Tg(Thy1-YFP)16Jrs/0
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J * CBA * SJL

mortality/aging

premature death ( J:237911 )

• accelerated in female and male mice compared with Tg(SOD1*G93A)1Gur mice

nervous system

abnormal neuromuscular synapse morphology ( J:237911 )

• fewer fully innervated neuromuscular junctions (NMJs) than in Tg(SOD1*G93A)1Gur mice with more partially and completely denervated NMJs

Genotype
MGI:5752648

cx4

• Allelic Composition: Gars1^C201R/Gars1⁺; Tg(Thy1-YFP)16Jrs/?
• Genetic Background: involves: BALB/cAnN * C3H/HeH * C57BL/6J * CBA

nervous system

abnormal neuromuscular synapse morphology ( J:229590 )

• at high magnification, but not evident at low magnification, approximately 5% of the neuromuscular junctions assesed in the levator auris longus at 4 months of age are found to be only partially innervated, far fewer than in Nmf249 heterozygotes

• nearly all terminals assessed in the levator auris longus show more diffuse postsynaptic staining, less distinct gutters, thinner axons and presynaptic nerves, synapses that are smaller than in wild-type controls, the average quantal content is significantly lower than normal, and the quantal content and mean evoked endplate currents are lower still at 4 months compared with 2 months

abnormal synaptic transmission ( J:229590 )

abnormal PNS synaptic transmission ( J:229590 )

• at 2 months of age 25% decrease in amplitude of evoked endplate currents, and quantal content in neuromuscular synapses of the levator auris longus muscle, and treatment with 3,4-DAP or physostigmine increases the in vivo evoked endplate currents at 4 months of age

• at 2 months of age the frequency of spontaneous release is consistently lower than normal although the quantal amplitude at synapses is normal, and no differences are found in the miniature endplate current amplitude

behavior/neurological

decreased grip strength ( J:229590 )

• compared with wild-type controls, heterozygotes have impaired grip strength and this is improved by treatment with physostigmine, but made worse by treatment with 3,4-DAP

Genotype
MGI:6466700

cx5

• Allelic Composition: Dynlrb1^{tm1a(EUCOMM)Wtsi}/Dynlrb1⁺; Tg(Thy1-YFP)16Jrs/0
• Genetic Background: involves: C57BL/6J * C57BL/6N
• Cell Lines: EPD0373_7_C01

nervous system

abnormal axon extension ( J:292124 )

• DRG neurons exhibit significantly reduced axon outgrowth after 48 h in culture

cellular

abnormal axon extension ( J:292124 )

• DRG neurons exhibit significantly reduced axon outgrowth after 48 h in culture

Genotype
MGI:5752579

cx6

• Allelic Composition: Gars1^Nmf249/Gars1⁺; Tg(Thy1-YFP)16Jrs/?
• Genetic Background: involves: C57BL/6J * CAST/Ei * CBA/J

nervous system

abnormal neuromuscular synapse morphology ( J:229590 )

• nearly all terminals assessed in the levator auris longus show more diffuse postsynaptic staining, less distinct gutters, thinner axons and presynaptic nerves, synapses that are smaller than in wild-type controls, fewer mitochondria at the synaptic terminals, and the average quantal content is significantly lower than normal

• at high magnification, but not evident at low magnification, approximately half of the junctions assesed in the levator auris longus at 2 months of age are found to be only partially innervated, even in the mildly affected mice, and there are more than 3 times as many denervated junctions at 2 months of age as are found in carriers of the C201R allele at 4 months of age

abnormal synaptic transmission ( J:229590 )

abnormal PNS synaptic transmission ( J:229590 )

• at 2 months of age 25% decrease in amplitude of evoked endplate currents, and quantal content in neuromuscular synapses the levator auris longus muscle

• at 2 months of age the frequency of spontaneous release is consistently lower than normal although the quantal amplitude at synapses is normal, and no differences are found in the miniature endplate current amplitude

abnormal endplate potential ( J:229590 )

behavior/neurological

decreased grip strength ( J:229590 )

• compared with wild-type controls, heterozygotes have impaired grip strength and this is improved by treatment with physostigmine