All Phenotypes Lat<tm4.1Mal> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lat^tm4.1Mal/Lat^tm4.1Mal	involves: 129P2/OlaHsd	MGI:3691075
cn2	Lat^tm4.1Mal/Lat^{tm6.1(HBEGF/EGFP)Mal}	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:4357975
cx3	Lat^tm4.1Mal/Lat^tm4.1Mal Trdc^tm1Mal/Trdc^tm1Mal	involves: 129P2/OlaHsd * C57BL/6	MGI:3690995
cx4	Lat^tm4.1Mal/Lat^tm4.1Mal Tg(Lck*F505)3073Rmp/?	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:4357976

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymus cell ratio ( J:94074 )

• thymi contain CD3+ gamma/delta TCR+ cells, in contrast to some published reports

abnormal thymocyte activation ( J:94074 )

• thymocytes are unable to be activated by anti-CD3epsilon antibody treatment

abnormal lymphocyte morphology ( J:94074 )

abnormal gamma-delta T cell differentiation ( J:94074 )

• 5.3-fold reduction in numbers found in the thymus

arrested T cell differentiation ( J:94074 )

• there is a strict developmental block in double negative T cells at the transition from the CD44-negative,CD25-positive stage to the CD44-, CD25- negative stage

decreased T cell number ( J:94074 )

• fewer gamma/delta T cells are present compared to wild-type thymi; progeny of these cells are subsequently lost before reaching periphery

decreased double-positive T cell number ( J:94074 )

• double-positive T cells are completely absent

absent CD4-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent CD8-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent mature gamma-delta T cells ( J:94074 )

• no gamma-delta T cells are found in the periphery

abnormal splenic cell ratio ( J:94074 )

• spleens lack gamma/delta T cells

abnormal lymph node cell ratio ( J:94074 )

• lymph nodes lack gamma/delta T cells

hematopoietic system

abnormal thymus cell ratio ( J:94074 )

• thymi contain CD3+ gamma/delta TCR+ cells, in contrast to some published reports

abnormal thymocyte activation ( J:94074 )

• thymocytes are unable to be activated by anti-CD3epsilon antibody treatment

abnormal lymphocyte morphology ( J:94074 )

abnormal gamma-delta T cell differentiation ( J:94074 )

• 5.3-fold reduction in numbers found in the thymus

arrested T cell differentiation ( J:94074 )

• there is a strict developmental block in double negative T cells at the transition from the CD44-negative,CD25-positive stage to the CD44-, CD25- negative stage

decreased T cell number ( J:94074 )

• fewer gamma/delta T cells are present compared to wild-type thymi; progeny of these cells are subsequently lost before reaching periphery

decreased double-positive T cell number ( J:94074 )

• double-positive T cells are completely absent

absent CD4-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent CD8-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent mature gamma-delta T cells ( J:94074 )

• no gamma-delta T cells are found in the periphery

abnormal splenic cell ratio ( J:94074 )

• spleens lack gamma/delta T cells

decreased platelet aggregation ( J:125744 )

• platelets do not aggregate in response to low doses of a snake venom clotting agonist or collagen

homeostasis/metabolism

decreased platelet aggregation ( J:125744 )

• platelets do not aggregate in response to low doses of a snake venom clotting agonist or collagen

abnormal thrombosis ( J:125744 )

• platelets are unable to form a multilayered thrombi on collagen coated surfaces

• the volume of thrombi are reduced by more than 2.6-fold and the surface area covered is reduced by 1.8-fold

endocrine/exocrine glands

abnormal thymus cell ratio ( J:94074 )

• thymi contain CD3+ gamma/delta TCR+ cells, in contrast to some published reports

abnormal thymocyte activation ( J:94074 )

• thymocytes are unable to be activated by anti-CD3epsilon antibody treatment

Allelic Composition	Lat^tm4.1Mal/Lat^{tm6.1(HBEGF/EGFP)Mal}
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lat^tm4.1Mal mutation (0 available); any Lat mutation (10 available)
Lat^{tm6.1(HBEGF/EGFP)Mal} mutation (1 available); any Lat mutation (10 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T-helper 2 physiology ( J:151840 )

• CD4⁺ T cells were first transfected with a cre recombinase vector and then treated with dipthera toxin so that surviving T cells only expressed the mutant Lat^tm1Mal allele

• when activated and transferred into T cell deficient hosts, these T cells cause splenomegaly, lymphadenopathy and secrete high levels of IL-4

• these abnormal T cells promote B cell activation in their hosts leading to high levels of IgG1 and IgE

hematopoietic system

abnormal T-helper 2 physiology ( J:151840 )

• CD4⁺ T cells were first transfected with a cre recombinase vector and then treated with dipthera toxin so that surviving T cells only expressed the mutant Lat^tm1Mal allele

• when activated and transferred into T cell deficient hosts, these T cells cause splenomegaly, lymphadenopathy and secrete high levels of IL-4

• these abnormal T cells promote B cell activation in their hosts leading to high levels of IgG1 and IgE

Allelic Composition	Lat^tm4.1Mal/Lat^tm4.1Mal Trdc^tm1Mal/Trdc^tm1Mal
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lat^tm4.1Mal mutation (0 available); any Lat mutation (10 available)
Trdc^tm1Mal mutation (1 available); any Trdc mutation (7 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal double-negative T cell morphology ( J:112658 )

• surface expression of Tcr gamma/delta heterodimers is normal, but stage 3 double negative (DN3) T cells are unable to mature beyond the EGFP^int Tcr gamma/delta^lo stage

• mature gamma/delta T cells are absent

• pool of EGFP^int Tcr gamma/delta^lo DN3 cells comprises ~0.5% of total DN3 cells

immune system

abnormal double-negative T cell morphology ( J:112658 )

• surface expression of Tcr gamma/delta heterodimers is normal, but stage 3 double negative (DN3) T cells are unable to mature beyond the EGFP^int Tcr gamma/delta^lo stage

• mature gamma/delta T cells are absent

• pool of EGFP^int Tcr gamma/delta^lo DN3 cells comprises ~0.5% of total DN3 cells

Allelic Composition	Lat^tm4.1Mal/Lat^tm4.1Mal Tg(Lck*F505)3073Rmp/?
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lat^tm4.1Mal mutation (0 available); any Lat mutation (10 available)
Tg(Lck*F505)3073Rmp mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

spleen hyperplasia ( J:151840 )

• spleen cellularity is increased 3-fold compared to transgenics on a wild-type background

increased CD4-positive, alpha-beta T cell number ( J:151840 )

• CD4⁺CD5⁺ T cell numbers are increased over 5-fold compared to controls

increased IgE level ( J:151840 )

• serum IgE levels are about 10,000-fold higher than controls

increased IgG1 level ( J:151840 )

• serum IgG1 levels are more than 100-fold higher than controls

abnormal T-helper 2 physiology ( J:151840 )

• transgene allows rescue of deficient thymocyte development of Lat-null mice

• 60% of these T cells express IL-4 upon PMA stimulation

• Th2 T cells hyper proliferate and stimulate immunoglobulin production

increased interleukin-4 secretion ( J:151840 )

• 60% of peripheral T cells express IL-4 upon PMA stimulation

hematopoietic system

spleen hyperplasia ( J:151840 )

• spleen cellularity is increased 3-fold compared to transgenics on a wild-type background

increased CD4-positive, alpha-beta T cell number ( J:151840 )

• CD4⁺CD5⁺ T cell numbers are increased over 5-fold compared to controls

increased IgE level ( J:151840 )

• serum IgE levels are about 10,000-fold higher than controls

increased IgG1 level ( J:151840 )

• serum IgG1 levels are more than 100-fold higher than controls

abnormal T-helper 2 physiology ( J:151840 )

• transgene allows rescue of deficient thymocyte development of Lat-null mice

• 60% of these T cells express IL-4 upon PMA stimulation

• Th2 T cells hyper proliferate and stimulate immunoglobulin production

growth/size/body

spleen hyperplasia ( J:151840 )

• spleen cellularity is increased 3-fold compared to transgenics on a wild-type background

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