Phenotypes associated with this allele

• Allele Symbol: Nrp1^tm2Ddg
• Allele Name: targeted mutation 2, David D Ginty
• Allele ID: MGI:3512101
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nrp1^tm2Ddg/Nrp1^tm2.1Ddg Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6	MGI:3512130
cn2	Nrp1^tm2Ddg/Nrp1^tm2Ddg Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6	MGI:3512126
cn3	Nrp1^tm2Ddg/Nrp1^tm2Ddg Nrp2^tm1Ddg/Nrp2^tm1Ddg Tg(Prrx1-cre)1Cjt/0	involves: 129 * C57BL/6J * SJL/J	MGI:3840961
cn4	Nrp1^tm2Ddg/Nrp1^tm2Ddg Or8a1^tm28(cre)Mom/Or8a1^tm28(cre)Mom Tg(CAG-Bgeo/GFP)21Lbe/?	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:6117236
cn5	Nrp1^tm1Hfu/Nrp1^tm2Ddg Tg(Tek-cre)1Ywa/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * SJL	MGI:5432163
cn6	Nrp1^tm2Ddg/Nrp1^tm2.1Ddg Tg(Gucy2g-cre,-EGFP)52Irod/0	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5431517

Genotype
MGI:3512130

cn1

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2.1Ddg; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:93799 )

• mid-to-late embryonic lethality

cardiovascular system

abnormal blood vessel morphology ( J:93799 )

• systemic vascular deficiencies at E12.5 such as distended vessels in the abdominal wall

• brain vessels were large and underdeveloped

failure of vascular branching ( J:93799 )

• lack of medium and small-diameter branched vessels in the abdominal wall and brain vessels were not branched

persistent truncus arteriosus ( J:93799 )

dilated heart atrium ( J:93799 )

• exhibited bilateral atrial enlargement

Genotype
MGI:3512126

cn2

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2Ddg; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

perinatal lethality, complete penetrance ( J:93799 )

• 25/25 mutant mice died perinatally

cardiovascular system

abnormal cardiovascular system morphology ( J:93799 )

• exhibit multiple cardiac defects

persistent truncus arteriosus ( J:93799 )

abnormal coronary vessel morphology ( J:93799 )

• misplacement of coronary arteries

ventricular septal defect ( J:93799 )

Genotype
MGI:3840961

cn3

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2Ddg; Nrp2^tm1Ddg/Nrp2^tm1Ddg; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: 129 * C57BL/6J * SJL/J

cardiovascular system

cardiovascular system phenotype ( J:147285 )

N • no major abnormalities in limb vascular patterning are detected at E13.5

Genotype
MGI:6117236

cn4

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2Ddg; Or8a1^tm28(cre)Mom/Or8a1^tm28(cre)Mom; Tg(CAG-Bgeo/GFP)21Lbe/?
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J

nervous system

abnormal olfactory bulb development ( J:255954 )

• olfactory sensory neurons expressing the null allele of Nrp1 have an altered and variable pattern of glomeruli formation in the olfactory bulb, including ectopic glomeruli, but the labeled glomeruli do not show an anterior shift relative to Nrp1 wild type controls

Genotype
MGI:5432163

cn5

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm2Ddg; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * SJL

nervous system

abnormal brain vasculature morphology ( J:175836 )

• fewer and larger

decreased gonadotropin-releasing hormone neuron number ( J:175836 )

• reduced gonadotropin-releasing hormone neurons

endocrine/exocrine glands

decreased gonadotropin-releasing hormone neuron number ( J:175836 )

• reduced gonadotropin-releasing hormone neurons

cardiovascular system

abnormal brain vasculature morphology ( J:175836 )

• fewer and larger

Genotype
MGI:5431517

cn6

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2.1Ddg; Tg(Gucy2g-cre,-EGFP)52Irod/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

nervous system

nervous system phenotype ( J:185650 )

N • in the olfactory bulb, Grueneberg glomeruli formation is not affected and their exclusion from guanylate cyclase G (GC-G) glomeruli is not affected

abnormal accessory olfactory bulb morphology ( J:185650 )

• numbers of Grueneberg axonal bundles consisting of multiple axons do not avoid the accessory olfactory bulb as seen in control animals; axonal fibers and microglomeruli cross bulb from medial to lateral side

• some Grueneberg fibers end deep into the vomeronasal glomeruli primarily in rostral part (not seen in controls)

• Grueneberg glomeruli form normally in mice with specific Nrp2 deletion and Grueneberg axons lacking Nrp2 do not cross the accessory olfactory bulb