Phenotypes associated with this allele

• Allele Symbol: Nrp1^tm2.1Ddg
• Allele Name: targeted mutation 2.1, David D Ginty
• Allele ID: MGI:3512103
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nrp1^tm2.1Ddg/Nrp1^tm2.1Ddg	involves: 129 * C57BL/6	MGI:3512122
cn2	Nrp1^tm2Ddg/Nrp1^tm2.1Ddg Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6	MGI:3512130
cn3	Nrp1^tm2Ddg/Nrp1^tm2.1Ddg Tg(Gucy2g-cre,-EGFP)52Irod/0	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5431517

Genotype
MGI:3512122

hm1

• Allelic Composition: Nrp1^tm2.1Ddg/Nrp1^tm2.1Ddg
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal trigeminal nerve morphology ( J:93799 )

• distal axons of the ophthalmic nerve were more defasciculated and disorganized than those in Nrp1^tm1Ddg

abnormal spinal nerve morphology ( J:93799 )

• spinal nerves were disorganized in E11.5 mutants

Genotype
MGI:3512130

cn2

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2.1Ddg; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

prenatal lethality, complete penetrance ( J:93799 )

• mid-to-late embryonic lethality

cardiovascular system

abnormal blood vessel morphology ( J:93799 )

• systemic vascular deficiencies at E12.5 such as distended vessels in the abdominal wall

• brain vessels were large and underdeveloped

failure of vascular branching ( J:93799 )

• lack of medium and small-diameter branched vessels in the abdominal wall and brain vessels were not branched

persistent truncus arteriosus ( J:93799 )

dilated heart atrium ( J:93799 )

• exhibited bilateral atrial enlargement

Genotype
MGI:5431517

cn3

• Allelic Composition: Nrp1^tm2Ddg/Nrp1^tm2.1Ddg; Tg(Gucy2g-cre,-EGFP)52Irod/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

nervous system

nervous system phenotype ( J:185650 )

N • in the olfactory bulb, Grueneberg glomeruli formation is not affected and their exclusion from guanylate cyclase G (GC-G) glomeruli is not affected

abnormal accessory olfactory bulb morphology ( J:185650 )

• numbers of Grueneberg axonal bundles consisting of multiple axons do not avoid the accessory olfactory bulb as seen in control animals; axonal fibers and microglomeruli cross bulb from medial to lateral side

• some Grueneberg fibers end deep into the vomeronasal glomeruli primarily in rostral part (not seen in controls)

• Grueneberg glomeruli form normally in mice with specific Nrp2 deletion and Grueneberg axons lacking Nrp2 do not cross the accessory olfactory bulb