Phenotypes associated with this allele

• Allele Symbol: Bin1^tm2Gcp
• Allele Name: targeted mutation 2, George C Pendergast
• Allele ID: MGI:3512133
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Bin1^tm1Gcp/Bin1^tm2Gcp Tg(MMTV-Myc)141-3Led/0 Tg(Wap-cre)11738Mam/0	involves: 129S6/SvEvTac * C57BL/6 * CD-1 * FVB/N * SJL	MGI:3697474
cn2	Bin1^tm1Gcp/Bin1^tm2Gcp Tg(Wap-cre)11738Mam/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL	MGI:3793422
cn3	Bin1^tm2Gcp/Bin1^+ Tg(Wap-cre)11738Mam/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL	MGI:3793426

Genotype
MGI:3697474

cn1

• Allelic Composition: Bin1^tm1Gcp/Bin1^tm2Gcp; Tg(MMTV-Myc)141-3Led/0; Tg(Wap-cre)11738Mam/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CD-1 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased mammary adenocarcinoma incidence ( J:117330 )

increased lymphoma incidence ( J:117330 )

• some mice develop aggressive lymphomas which occasionally appear before mammary carcinomas

cellular

abnormal cell physiology ( J:117330 )

• tumor cells show increased motility in monolayer culture

decreased apoptosis ( J:117330 )

• tumor cells in culture show several fold greater resistance to serum deprivation-induced apoptosis

increased cell proliferation ( J:117330 )

• tumor cells show 3-4 fold higher rates of proliferation under anchorage-dependent conditions

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:117330 )

• tumor cells have higher gelatinase activity

endocrine/exocrine glands

increased mammary adenocarcinoma incidence ( J:117330 )

integument

increased mammary adenocarcinoma incidence ( J:117330 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
breast cancer		DOID:1612	OMIM:114480	J:117730

Genotype
MGI:3793422

cn2

• Allelic Composition: Bin1^tm1Gcp/Bin1^tm2Gcp; Tg(Wap-cre)11738Mam/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL

endocrine/exocrine glands

abnormal involution of the mammary gland ( J:117330 )

♀ • ductolobular regression was achieved with somewhat slower kinetics in mutant mice during glandular involution after pregnancy

abnormal mammary gland embryonic development ( J:117330 )

♀ • a delay in the kinetics of ductolobular development was apparent at 18.5 dpc, when mutant mice showed significantly less glandular remodeling

♀ • at 7.5 dpp, this defect had resolved, such that no deficiencies were apparent in nursing and pups showed no signs of malnutrition

increased mammary gland tumor incidence ( J:117330 )

• 8% of elderly animals of >1 year of age exhibited developed poorly differentiated tumors

• DMBA treated animals developed poorly differentiated tumors, characterized by low tubule formation, high mitotic indices, and high degrees of nuclear pleomorphism and relative increase in lymphocyte infiltration compared with mice carrying one wild-type allele of Bin1

increased granulosa cell tumor incidence ( J:117330 )

♀ • ovarian granulosa cell tumors are noted

♀ • increased incidence of DMBA-induced ovarian granulosa cell tumors in mutant mice (43%) relative to mice carrying one wild-type copy of Bim1 (13%), all of whom also had mammary tumors

neoplasm

increased mammary gland tumor incidence ( J:117330 )

• 8% of elderly animals of >1 year of age exhibited developed poorly differentiated tumors

• DMBA treated animals developed poorly differentiated tumors, characterized by low tubule formation, high mitotic indices, and high degrees of nuclear pleomorphism and relative increase in lymphocyte infiltration compared with mice carrying one wild-type allele of Bin1

increased granulosa cell tumor incidence ( J:117330 )

♀ • ovarian granulosa cell tumors are noted

♀ • increased incidence of DMBA-induced ovarian granulosa cell tumors in mutant mice (43%) relative to mice carrying one wild-type copy of Bim1 (13%), all of whom also had mammary tumors

increased incidence of tumors by chemical induction ( J:117330 )

• DMBA treated animals developed poorly differentiated tumors, characterized by low tubule formation, high mitotic indices, and high degrees of nuclear pleomorphism and relative increase in lymphocyte infiltration compared with mice carrying one wild-type allele of Bin1

• increased incidence of DMBA-induced ovarian granulosa cell tumors in mutant mice (43%) relative to mice carrying one wild-type copy of Bim1 (13%), all of whom also had mammary tumors

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:117330 )

• DMBA treated animals developed poorly differentiated tumors, characterized by low tubule formation, high mitotic indices, and high degrees of nuclear pleomorphism and relative increase in lymphocyte infiltration compared with mice carrying one wild-type allele of Bin1

• increased incidence of DMBA-induced ovarian granulosa cell tumors in mutant mice (43%) relative to mice carrying one wild-type copy of Bim1 (13%), all of whom also had mammary tumors

immune system

endometrium inflammation ( J:117330 )

• elevation in uterine endometritis

integument

abnormal involution of the mammary gland ( J:117330 )

♀ • ductolobular regression was achieved with somewhat slower kinetics in mutant mice during glandular involution after pregnancy

abnormal mammary gland embryonic development ( J:117330 )

♀ • a delay in the kinetics of ductolobular development was apparent at 18.5 dpc, when mutant mice showed significantly less glandular remodeling

♀ • at 7.5 dpp, this defect had resolved, such that no deficiencies were apparent in nursing and pups showed no signs of malnutrition

increased mammary gland tumor incidence ( J:117330 )

• 8% of elderly animals of >1 year of age exhibited developed poorly differentiated tumors

• DMBA treated animals developed poorly differentiated tumors, characterized by low tubule formation, high mitotic indices, and high degrees of nuclear pleomorphism and relative increase in lymphocyte infiltration compared with mice carrying one wild-type allele of Bin1

reproductive system

increased granulosa cell tumor incidence ( J:117330 )

♀ • ovarian granulosa cell tumors are noted

♀ • increased incidence of DMBA-induced ovarian granulosa cell tumors in mutant mice (43%) relative to mice carrying one wild-type copy of Bim1 (13%), all of whom also had mammary tumors

endometrium inflammation ( J:117330 )

• elevation in uterine endometritis

Genotype
MGI:3793426

cn3

• Allelic Composition: Bin1^tm2Gcp/Bin1⁺; Tg(Wap-cre)11738Mam/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL

neoplasm

increased mammary gland tumor incidence ( J:117330 )

• 5% of elderly animals of >1 year of age developed mammary tumors

• DMBA-treated animals developed well-differentiated tumors, characterized by high tubule formation, low mitotic indices, and limited nuclear pleomorphism

increased incidence of tumors by chemical induction ( J:117330 )

• DMBA-treated animals developed well-differentiated tumors, characterized by high tubule formation, low mitotic indices, and limited nuclear pleomorphism

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:117330 )

• DMBA-treated animals developed well-differentiated tumors, characterized by high tubule formation, low mitotic indices, and limited nuclear pleomorphism

endocrine/exocrine glands

increased mammary gland tumor incidence ( J:117330 )

• 5% of elderly animals of >1 year of age developed mammary tumors

• DMBA-treated animals developed well-differentiated tumors, characterized by high tubule formation, low mitotic indices, and limited nuclear pleomorphism

integument

increased mammary gland tumor incidence ( J:117330 )

• 5% of elderly animals of >1 year of age developed mammary tumors

• DMBA-treated animals developed well-differentiated tumors, characterized by high tubule formation, low mitotic indices, and limited nuclear pleomorphism